REMARK SOURCE hebe.pdb HEADER STRUCTURAL GENOMICS, UNKNOWN FUNCTION 18-JUN-09 3HWU COMPND PUTATIVE DNA-BINDING PROTEIN; COMPND 3 CHAIN: A; COMPND 4 FRAGMENT: SEQUENCE DATABASE RESIDUES 28-173; COMPND 5 ENGINEERED: YES SOURCE MOL_ID: 1; SOURCE 2 ORGANISM_SCIENTIFIC: RALSTONIA EUTROPHA; SOURCE 3 ORGANISM_TAXID: 264198; SOURCE 4 STRAIN: JMP134; SOURCE 5 GENE: REUT_B5223, YP_299413.1; SOURCE 6 EXPRESSION_SYSTEM: ESCHERICHIA COLI; SOURCE 7 EXPRESSION_SYSTEM_TAXID: 562; SOURCE 8 EXPRESSION_SYSTEM_STRAIN: HK100; SOURCE 9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID; SOURCE 10 EXPRESSION_SYSTEM_PLASMID: SPEEDET AUTHOR JOINT CENTER FOR STRUCTURAL GENOMICS (JCSG) HET MSE A 35 8 HET MSE A 43 16 HET MSE A 75 8 HET MSE A 100 8 HET MSE A 103 8 >GLU.A 29 1.97 14.29 77.87 1.82 15.14 75.87 1.89 14.72 76.87 2 0 1000.00 -999 ? >LEU.A 30 5.89 12.23 80.37 6.78 13.76 79.14 6.33 12.99 79.76 3 0 1000.00 -999 ? >GLY.A 32 7.21 5.55 81.34 7.24 6.28 81.08 7.23 5.92 81.21 1 0 1000.00 -999 ? >LYS.A 33 9.14 7.88 79.04 5.51 8.16 74.66 7.32 8.02 76.85 2 0 1000.00 -999 ? >TYR^A 34 10.83 11.39 82.35 9.08 12.72 84.02 9.95 12.05 83.18 3 0 1000.00 -999 ? >MET?A 35 13.79 8.50 84.60 16.69 5.31 84.28 15.24 6.91 84.44 2 0 1000.00 -999 ? >LYS^A 36 15.53 10.80 87.07 18.01 13.57 90.92 16.77 12.18 88.99 2 0 1000.00 -999 ? >THR^A 37 19.32 10.63 86.94 19.68 10.89 84.86 19.50 10.76 85.90 2 0 1000.00 -999 ? >PRO.A 38 22.92 10.05 89.46 21.56 11.75 88.81 22.24 10.90 89.14 3 0 1000.00 -999 ? >THR.A 39 22.50 15.05 86.83 24.20 14.29 85.73 23.35 14.67 86.28 2 0 1000.00 -999 ? >GLY^A 40 19.03 15.42 85.31 19.10 14.61 85.53 19.06 15.02 85.42 1 0 1000.00 -999 ? >TYR^A 41 14.85 14.31 84.03 13.70 15.74 86.13 14.27 15.02 85.08 3 0 1000.00 -999 ? >LEU^A 42 17.84 8.87 81.00 18.78 7.58 79.48 18.31 8.23 80.24 3 0 1000.00 -999 ? >MET?A 43 14.22 10.05 78.28 12.23 13.76 77.32 13.23 11.91 77.80 2 0 1000.00 -999 ? >VAL^A 44 13.32 7.24 75.93 14.23 5.24 75.99 13.78 6.24 75.96 3 0 1000.00 -999 ? >LEU^A 45 10.72 9.67 72.72 10.40 11.58 73.77 10.56 10.62 73.24 3 0 1000.00 -999 ? >ARG^A 46 9.66 3.73 73.42 8.48 1.10 74.27 9.07 2.42 73.84 2 0 1000.00 -999 ? >HIS.A 47 5.73 3.35 67.69 4.21 2.18 68.56 4.97 2.76 68.12 3 0 1000.00 -999 ? >GLY.A 48 5.13 8.40 66.90 5.72 8.50 67.43 5.43 8.45 67.16 1 0 1000.00 -999 ? >ASP.A 49 7.24 10.35 70.89 6.31 9.06 72.31 6.78 9.71 71.60 2 0 1000.00 -999 ? >ASN~A 50 6.01 15.42 68.37 4.55 15.01 66.89 5.28 15.21 67.63 2 0 1000.00 -999 ? >VAL~A 51 10.40 14.97 70.18 12.34 14.07 69.61 11.37 14.52 69.90 3 0 1000.00 -999 ? >LEU~A 52 10.74 19.89 69.93 12.66 20.20 68.95 11.70 20.05 69.44 3 0 1000.00 -999 ? >GLN~A 53 5.32 19.26 70.29 3.66 18.76 69.05 4.49 19.01 69.67 2 0 1000.00 -999 ? >ASN~A 54 8.21 14.41 74.26 6.54 13.14 73.99 7.38 13.78 74.13 2 0 1000.00 -999 ? >LEU~A 55 12.02 18.37 74.97 13.89 17.26 74.76 12.95 17.81 74.87 3 0 1000.00 -999 ? >GLU~A 56 8.26 22.27 74.17 7.12 22.77 72.44 7.69 22.52 73.31 2 0 1000.00 -999 ? >GLN~A 57 4.32 16.99 78.82 2.84 18.46 79.16 3.58 17.72 78.99 2 0 1000.00 -999 ? >LEU~A 58 10.40 16.72 79.94 11.92 15.61 81.08 11.16 16.16 80.51 3 0 1000.00 -999 ? >ALA~A 59 10.05 21.00 81.14 10.41 22.07 80.07 10.23 21.54 80.61 2 0 1000.00 -999 ? >ARG~A 60 3.72 22.48 79.72 4.09 22.76 76.65 3.90 22.62 78.19 2 0 1000.00 -999 ? >ASP~A 61 5.85 17.06 83.77 4.15 16.72 82.58 5.00 16.89 83.18 2 0 1000.00 -999 ? >GLU~A 62 10.18 16.27 85.48 9.94 15.28 87.35 10.06 15.77 86.42 2 0 1000.00 -999 ? >HIS.A 63 7.66 22.28 88.00 5.83 21.33 88.35 6.75 21.81 88.18 3 0 1000.00 -999 ? >ILE.A 64 13.35 21.65 83.39 14.12 20.64 82.68 13.73 21.14 83.03 3 0 1000.00 -999 ? >PRO.A 65 15.09 24.87 87.62 14.49 25.01 85.45 14.79 24.94 86.54 3 0 1000.00 -999 ? >SER^A 66 17.03 25.24 82.44 19.02 26.41 83.32 18.02 25.83 82.88 2 0 1000.00 -999 ? >ALA^A 67 18.90 22.31 80.91 17.92 21.11 80.93 18.41 21.71 80.92 2 0 1000.00 -999 ? >SER^A 68 21.23 21.01 78.24 23.41 20.85 77.17 22.32 20.93 77.70 2 0 1000.00 -999 ? >PHE^A 69 18.36 18.34 75.13 16.99 19.84 73.23 17.67 19.09 74.18 3 0 1000.00 -999 ? >VAL^A 70 21.99 15.44 73.66 24.09 15.16 74.27 23.04 15.30 73.96 3 0 1000.00 -999 ? >GLY^A 71 20.25 12.51 71.95 20.78 12.78 71.41 20.52 12.65 71.68 1 0 1000.00 -999 ? >ILE^A 72 21.07 8.81 69.16 22.10 8.00 69.70 21.59 8.41 69.43 3 0 1000.00 -999 ? >GLY^A 73 17.17 8.01 67.50 17.63 8.32 66.88 17.40 8.16 67.19 1 0 1000.00 -999 ? >PHE^A 74 14.71 5.33 62.61 13.95 4.88 60.01 14.33 5.11 61.31 3 0 1000.00 -999 ? >MET?A 75 12.80 9.60 62.88 13.15 11.51 65.59 12.97 10.56 64.23 2 0 1000.00 -999 ? >SER^A 76 9.85 9.14 60.54 7.90 9.66 61.87 8.88 9.40 61.20 2 0 1000.00 -999 ? >GLU^A 77 7.77 13.10 58.82 6.21 14.42 58.40 6.99 13.76 58.61 2 0 1000.00 -999 ? >ALA^A 78 12.71 15.56 60.78 14.12 15.08 61.05 13.42 15.32 60.92 2 0 1000.00 -999 ? >THR^A 79 12.19 19.18 59.69 10.25 19.88 59.04 11.22 19.53 59.36 2 0 1000.00 -999 ? >PHE^A 80 16.79 19.97 59.82 17.58 17.61 61.03 17.18 18.79 60.42 3 0 1000.00 -999 ? >GLY^A 81 14.97 25.45 59.06 15.74 25.17 59.28 15.35 25.31 59.17 1 0 1000.00 -999 ? >PHE^A 82 19.75 28.26 56.50 22.23 29.53 56.54 20.99 28.90 56.52 3 0 1000.00 -999 ? >TYR^A 83 16.00 32.97 60.75 14.27 34.99 61.55 15.14 33.98 61.15 3 0 1000.00 -999 ? >ASP.A 84 15.58 34.14 55.21 15.93 35.76 53.97 15.76 34.95 54.59 2 0 1000.00 -999 ? >PHE.A 85 18.82 37.20 59.87 19.88 35.57 61.88 19.35 36.38 60.88 3 0 1000.00 -999 ? >GLY.A 86 15.56 39.61 55.43 15.15 39.07 55.85 15.35 39.34 55.64 1 0 1000.00 -999 ? >ARG.A 87 11.70 35.33 52.95 13.58 32.96 52.49 12.64 34.14 52.72 2 0 1000.00 -999 ? >LYS.A 88 12.35 36.86 59.22 10.33 42.31 60.33 11.34 39.59 59.78 2 0 1000.00 -999 ? >GLN^A 89 9.19 34.18 56.99 8.19 34.30 55.14 8.69 34.24 56.06 2 0 1000.00 -999 ? >PHE^A 90 13.28 29.11 60.69 14.35 29.69 63.19 13.81 29.40 61.94 3 0 1000.00 -999 ? >ASP^A 91 14.39 28.34 53.86 14.04 27.24 52.05 14.22 27.79 52.96 2 0 1000.00 -999 ? >PRO^A 92 10.08 25.10 55.09 12.28 25.25 55.55 11.18 25.18 55.32 3 0 1000.00 -999 ? >THR^A 94 11.90 17.76 55.71 9.85 17.48 56.37 10.87 17.62 56.04 2 0 1000.00 -999 ? >TYR^A 95 16.05 15.27 55.67 17.78 17.05 54.41 16.92 16.16 55.04 3 0 1000.00 -999 ? >ARG^A 96 8.81 11.74 53.73 6.51 13.50 54.62 7.66 12.62 54.18 2 0 1000.00 -999 ? >ASN^A 97 12.29 6.73 56.71 10.24 6.23 57.06 11.27 6.48 56.89 2 0 1000.00 -999 ? >VAL^A 98 16.26 8.93 58.85 16.15 11.03 58.22 16.20 9.98 58.54 3 0 1000.00 -999 ? >GLU^A 99 19.59 5.57 61.29 18.90 4.59 63.04 19.25 5.08 62.16 2 0 1000.00 -999 ? >MET?A 100 19.42 10.92 63.87 17.57 13.88 66.28 18.49 12.40 65.08 2 0 1000.00 -999 ? >ALA^A 101 23.14 10.69 64.42 24.19 10.96 63.30 23.66 10.82 63.86 2 0 1000.00 -999 ? >ASN^A 102 24.42 14.11 67.13 24.50 15.53 68.76 24.46 14.82 67.95 2 0 1000.00 -999 ? >MET?A 103 20.80 16.04 68.57 16.61 16.51 69.64 18.70 16.28 69.10 2 0 1000.00 -999 ? >THR^A 104 22.24 18.78 70.81 24.41 18.98 70.86 23.32 18.88 70.83 2 0 1000.00 -999 ? >GLY^A 105 20.17 21.51 72.50 20.54 21.15 73.09 20.36 21.33 72.80 1 0 1000.00 -999 ? >SER^A 106 18.87 23.41 75.49 19.20 25.72 74.79 19.03 24.57 75.14 2 0 1000.00 -999 ? >ILE^A 107 15.16 22.81 78.48 14.17 21.70 78.83 14.67 22.26 78.66 3 0 1000.00 -999 ? >ALA^A 108 14.79 27.37 78.64 15.82 28.43 78.21 15.30 27.90 78.43 2 0 1000.00 -999 ? >TRP^A 109 12.43 27.98 81.56 9.09 25.93 81.69 10.76 26.96 81.63 3 0 1000.00 -999 ? >LYS^A 110 10.12 32.43 76.84 11.14 35.48 71.32 10.63 33.96 74.08 2 0 1000.00 -999 ? >GLU.A 111 7.01 35.80 75.74 6.00 36.44 74.02 6.51 36.12 74.88 2 0 1000.00 -999 ? >GLY.A 112 6.18 31.59 80.37 6.43 31.38 79.69 6.31 31.49 80.03 1 0 1000.00 -999 ? >PRO^A 114 7.56 24.49 77.94 8.45 26.48 77.51 8.01 25.49 77.73 3 0 1000.00 -999 ? >SER^A 115 11.54 27.26 74.68 12.66 29.17 73.67 12.10 28.22 74.17 2 0 1000.00 -999 ? >ILE^A 116 12.86 23.00 72.87 13.16 22.15 73.98 13.01 22.57 73.42 3 0 1000.00 -999 ? >HIS^A 117 18.43 26.75 70.47 19.82 27.51 69.11 19.13 27.13 69.79 3 0 1000.00 -999 ? >ALA^A 118 17.74 22.08 69.28 16.75 20.89 69.44 17.25 21.48 69.36 2 0 1000.00 -999 ? >HIS^A 119 21.38 23.30 66.12 21.58 25.37 65.88 21.48 24.34 66.00 3 0 1000.00 -999 ? >GLY^A 120 20.38 17.76 64.74 20.66 18.31 64.22 20.52 18.04 64.48 1 0 1000.00 -999 ? >THR^A 121 22.28 15.67 62.22 24.47 15.69 62.33 23.37 15.68 62.28 2 0 1000.00 -999 ? >VAL^A 122 20.34 12.98 60.21 18.38 14.01 60.03 19.36 13.50 60.12 3 0 1000.00 -999 ? >THR^A 123 21.43 10.35 57.72 23.12 9.62 58.88 22.28 9.99 58.30 2 0 1000.00 -999 ? >ASP.A 124 18.77 8.56 54.30 19.87 9.20 52.62 19.32 8.88 53.46 2 0 1000.00 -999 ? >GLY.A 125 20.52 4.85 53.34 20.77 5.58 53.17 20.64 5.22 53.25 1 0 1000.00 -999 ? >THR.A 126 23.17 6.86 51.48 22.38 8.68 50.64 22.78 7.77 51.06 2 0 1000.00 -999 ? >PHE.A 127 25.45 5.20 55.23 24.54 2.59 55.46 25.00 3.89 55.34 3 0 1000.00 -999 ? >GLN.A 128 25.75 11.23 51.42 25.09 11.63 49.42 25.42 11.43 50.42 2 0 1000.00 -999 ? >GLY^A 129 24.59 13.76 56.65 24.56 14.00 55.92 24.58 13.88 56.29 1 0 1000.00 -999 ? >ALA^A 130 22.18 16.67 56.76 21.01 16.45 55.76 21.60 16.56 56.26 2 0 1000.00 -999 ? >GLY^A 131 20.58 18.85 59.37 20.99 19.36 58.88 20.78 19.10 59.13 1 0 1000.00 -999 ? >GLY^A 132 20.89 22.09 61.29 20.38 21.53 61.63 20.63 21.81 61.46 1 0 1000.00 -999 ? >HIS^A 133 17.02 26.04 64.04 15.71 26.98 65.38 16.37 26.51 64.71 3 0 1000.00 -999 ? >LEU^A 134 15.37 20.58 65.27 16.03 18.52 65.04 15.70 19.55 65.16 3 0 1000.00 -999 ? >LEU^A 135 11.20 23.41 62.43 11.73 25.35 61.55 11.46 24.38 61.99 3 0 1000.00 -999 ? >GLY^A 136 10.20 19.54 64.63 10.87 19.40 64.10 10.54 19.47 64.36 1 0 1000.00 -999 ? >LEU^A 137 12.25 15.54 64.62 13.56 16.03 66.34 12.90 15.79 65.48 3 0 1000.00 -999 ? >THR^A 138 8.79 12.62 65.06 6.70 12.65 64.49 7.74 12.63 64.78 2 0 1000.00 -999 ? >VAL^A 139 10.10 9.22 66.17 10.69 8.44 68.15 10.40 8.83 67.16 3 0 1000.00 -999 ? >GLY^A 140 9.66 6.52 63.53 9.90 6.32 64.29 9.78 6.42 63.91 1 0 1000.00 -999 ? >THR^A 141 9.91 2.78 63.79 9.47 2.01 61.88 9.69 2.40 62.84 2 0 1000.00 -999 ? >GLY^A 142 12.52 3.11 66.53 11.75 3.42 66.79 12.14 3.27 66.66 1 0 1000.00 -999 ? >SER^A 143 13.10 5.23 69.63 14.88 3.93 70.65 13.99 4.58 70.14 2 0 1000.00 -999 ? >CYH^A 144 14.84 8.43 70.74 15.11 11.20 70.53 14.97 9.82 70.64 2 0 1000.00 -999 ? >GLU^A 145 18.07 6.03 73.62 19.71 4.72 73.51 18.89 5.37 73.57 2 0 1000.00 -999 ? >ILE^A 146 16.54 12.89 75.12 15.75 13.87 74.48 16.14 13.38 74.80 3 0 1000.00 -999 ? >THR^A 147 20.24 12.74 78.03 22.11 11.77 78.25 21.17 12.25 78.14 2 0 1000.00 -999 ? >VAL^A 148 19.12 15.89 79.91 17.01 16.55 79.75 18.06 16.22 79.83 3 0 1000.00 -999 ? >THR^A 149 21.43 17.82 82.31 23.56 17.80 82.21 22.49 17.81 82.26 2 0 1000.00 -999 ? >VAL^A 150 19.32 20.06 84.60 17.27 19.53 85.26 18.29 19.79 84.93 3 0 1000.00 -999 ? >TYR^A 151 21.91 23.84 84.07 23.49 22.76 82.07 22.70 23.30 83.07 3 0 1000.00 -999 ? >PRO.A 152 21.23 25.34 89.96 19.48 24.87 88.63 20.35 25.11 89.29 3 0 1000.00 -999 ? >GLN.A 153 21.24 29.34 87.73 22.70 27.83 87.44 21.97 28.59 87.58 2 0 1000.00 -999 ? >ARG.A 154 12.28 29.47 84.92 9.48 29.39 83.64 10.88 29.43 84.28 2 0 1000.00 -999 ? >LEU.A 155 17.85 30.29 81.57 19.85 30.18 82.36 18.85 30.23 81.97 3 0 1000.00 -999 ? >ASP.A 156 14.12 34.28 80.10 12.65 33.89 81.55 13.39 34.09 80.82 2 0 1000.00 -999 ? >ARG^A 157 15.56 30.28 73.56 17.32 27.93 74.33 16.44 29.11 73.94 2 0 1000.00 -999 ? >PHE^A 158 17.33 38.12 75.51 20.16 38.26 75.24 18.75 38.19 75.37 3 0 1000.00 -999 ? >VAL^A 159 15.59 38.65 71.24 14.16 39.05 69.66 14.87 38.85 70.45 3 0 1000.00 -999 ? >ASP.A 160 20.34 39.33 70.76 22.05 40.51 70.64 21.20 39.92 70.70 2 0 1000.00 -999 ? >PRO.A 161 18.70 44.47 71.27 18.53 42.93 69.65 18.61 43.70 70.46 3 0 1000.00 -999 ? >ILE.A 163 21.80 39.17 65.91 22.54 38.34 66.75 22.17 38.75 66.33 3 0 1000.00 -999 ? >GLN.A 164 17.23 42.07 64.06 15.68 43.49 64.46 16.45 42.78 64.26 2 0 1000.00 -999 ? >ALA.A 165 17.53 36.38 66.88 18.74 35.64 66.32 18.14 36.01 66.60 2 0 1000.00 -999 ? >ASN^A 166 15.63 33.47 70.27 13.66 34.18 70.11 14.64 33.82 70.19 2 0 1000.00 -999 ? >VAL^A 167 19.56 34.11 72.51 21.41 35.28 72.25 20.49 34.69 72.38 3 0 1000.00 -999 ? >LEU^A 168 20.38 30.93 76.06 19.83 29.46 77.57 20.10 30.19 76.81 3 0 1000.00 -999 ? >GLY.A 169 19.97 34.82 78.73 20.02 34.08 78.95 20.00 34.45 78.84 1 0 1000.00 -999 ? >LEU.A 170 23.90 34.20 81.43 24.99 32.60 80.46 24.45 33.40 80.94 3 0 1000.00 -999 ? >PRO.A 171 22.07 33.37 85.80 22.33 35.16 84.53 22.20 34.26 85.17 3 0 1000.00 -999 ?