REMARK SOURCE hebe.pdb HEADER STRUCTURAL GENOMICS, UNKNOWN FUNCTION 11-MAY-09 3HFI COMPND PUTATIVE REGULATOR; COMPND 3 CHAIN: A; COMPND 4 FRAGMENT: RESIDUES 82-251; COMPND 5 ENGINEERED: YES SOURCE MOL_ID: 1; SOURCE 2 ORGANISM_SCIENTIFIC: ESCHERICHIA COLI O6; SOURCE 3 ORGANISM_TAXID: 217992; SOURCE 4 STRAIN: CFT073; SOURCE 5 GENE: C4276, GI:26250098; SOURCE 6 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21; SOURCE 7 EXPRESSION_SYSTEM_TAXID: 511693; SOURCE 8 EXPRESSION_SYSTEM_STRAIN: BL21; SOURCE 9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID; SOURCE 10 EXPRESSION_SYSTEM_PLASMID: PMCSG7 AUTHOR R.ZHANG,X.XU,H.ZHENG,A.SAVCHENKO,A.EDWARDS,A.JOACHIMIAK,MIDWEST AUTHOR 2 CENTER FOR STRUCTURAL GENOMICS (MCSG) >THR.A 25 47.08 61.52 1.50 49.02 62.35 2.17 48.05 61.93 1.83 2 0 1000.00 -999 ? >THR^A 26 47.14 57.81 2.26 46.04 56.66 0.79 46.59 57.23 1.52 2 0 1000.00 -999 ? >GLU^A 27 50.49 57.26 6.04 50.79 59.17 6.88 50.64 58.22 6.46 2 0 1000.00 -999 ? >VAL^A 28 51.07 52.53 4.19 51.25 50.78 2.84 51.16 51.65 3.52 3 0 1000.00 -999 ? >ILE^A 29 50.85 51.94 8.99 50.20 52.50 10.10 50.53 52.22 9.55 3 0 1000.00 -999 ? >THR^A 30 52.46 48.18 7.52 52.31 46.83 9.26 52.39 47.51 8.39 2 0 1000.00 -999 ? >SER^A 31 51.86 45.10 5.51 53.23 45.14 3.51 52.55 45.12 4.51 2 0 1000.00 -999 ? >ARG^A 32 51.48 41.02 9.78 48.93 42.27 11.02 50.20 41.64 10.40 2 0 1000.00 -999 ? >ILE^A 33 51.99 37.16 3.55 51.38 37.31 2.36 51.69 37.24 2.96 3 0 1000.00 -999 ? >GLU^A 34 47.19 37.83 7.53 46.19 39.10 9.03 46.69 38.46 8.28 2 0 1000.00 -999 ? >PRO^A 35 48.46 32.10 8.48 47.30 33.55 7.18 47.88 32.82 7.83 3 0 1000.00 -999 ? >ALA.A 36 43.08 34.08 6.64 42.25 33.88 5.45 42.67 33.98 6.05 2 0 1000.00 -999 ? >ASN~A 37 41.22 34.31 11.23 40.04 36.07 11.15 40.63 35.19 11.19 2 0 1000.00 -999 ? >ARG~A 38 37.34 28.80 12.34 39.43 29.37 14.59 38.38 29.08 13.47 2 0 1000.00 -999 ? >TYR~A 39 34.91 37.17 10.68 33.07 39.25 10.34 33.99 38.21 10.51 3 0 1000.00 -999 ? >VAL~A 40 37.96 36.58 7.12 39.70 37.86 7.05 38.83 37.22 7.09 3 0 1000.00 -999 ? >ALA~A 41 38.04 33.38 5.09 38.63 31.98 5.53 38.33 32.68 5.31 2 0 1000.00 -999 ? >GLU~A 42 31.81 33.22 6.54 31.04 33.63 8.60 31.42 33.42 7.57 2 0 1000.00 -999 ? >LYS~A 43 33.92 36.82 4.06 30.61 39.68 6.90 32.26 38.25 5.48 2 0 1000.00 -999 ? >LEU~A 44 37.84 36.46 1.34 39.05 38.26 1.23 38.44 37.36 1.29 3 0 1000.00 -999 ? >ARG~A 45 31.61 33.85 -1.21 29.24 35.84 -1.67 30.43 34.85 -1.44 2 0 1000.00 -999 ? >ILE.A 46 39.65 32.08 0.56 40.36 33.38 0.55 40.00 32.73 0.55 3 0 1000.00 -999 ? >THR.A 47 39.73 28.23 2.70 40.32 26.28 2.04 40.03 27.25 2.37 2 0 1000.00 -999 ? >PRO.A 48 38.40 28.17 6.86 40.23 28.93 5.68 39.32 28.55 6.27 3 0 1000.00 -999 ? >GLY.A 49 44.37 29.45 6.55 44.00 29.78 5.91 44.18 29.61 6.23 1 0 1000.00 -999 ? >GLN.A 50 44.47 30.95 0.60 43.09 30.72 -0.94 43.78 30.83 -0.17 2 0 1000.00 -999 ? >ASP^A 51 48.98 33.21 2.30 50.29 32.34 3.70 49.63 32.77 3.00 2 0 1000.00 -999 ? >ILE^A 52 44.72 37.11 2.79 44.39 37.34 3.58 44.56 37.23 3.19 3 0 1000.00 -999 ? >LEU^A 53 48.13 40.94 0.79 49.66 42.43 0.46 48.90 41.68 0.62 3 0 1000.00 -999 ? >TYR^A 54 47.09 43.14 7.54 46.58 45.06 9.52 46.84 44.10 8.53 3 0 1000.00 -999 ? >LEU^A 55 47.28 46.78 3.13 48.29 46.66 1.28 47.79 46.72 2.21 3 0 1000.00 -999 ? >GLU^A 56 47.21 49.58 9.62 48.05 49.07 11.57 47.63 49.33 10.59 2 0 1000.00 -999 ? >ARG^A 57 46.28 50.79 2.34 43.97 52.11 0.75 45.12 51.45 1.55 2 0 1000.00 -999 ? >LEU^A 58 47.40 56.30 8.34 47.05 57.49 10.15 47.22 56.89 9.25 3 0 1000.00 -999 ? >ARG^A 59 41.52 58.63 2.91 41.52 56.32 0.88 41.52 57.47 1.90 2 0 1000.00 -999 ? >SER^A 60 44.77 61.97 6.04 47.19 62.23 5.91 45.98 62.10 5.97 2 0 1000.00 -999 ? >ILE.A 61 41.02 64.43 4.71 39.88 63.93 4.34 40.45 64.18 4.53 3 0 1000.00 -999 ? >GLY.A 62 44.34 68.07 4.30 44.62 67.46 4.75 44.48 67.77 4.53 1 0 1000.00 -999 ? >ASP.A 63 48.09 68.18 6.71 50.06 67.60 7.26 49.08 67.89 6.98 2 0 1000.00 -999 ? >GLU.A 64 42.65 66.31 11.04 40.55 66.62 11.13 41.60 66.47 11.09 2 0 1000.00 -999 ? >LYS^A 65 44.25 62.13 9.94 49.57 61.31 12.21 46.91 61.72 11.08 2 0 1000.00 -999 ? >ALA^A 66 40.83 61.01 8.64 40.43 61.13 7.15 40.63 61.07 7.89 2 0 1000.00 -999 ? >MET^A 67 40.78 57.23 8.89 38.66 56.73 5.33 39.72 56.98 7.11 2 0 1000.00 -999 ? >LEU^A 68 43.10 53.66 10.96 44.74 52.56 11.70 43.92 53.11 11.33 3 0 1000.00 -999 ? >ILE^A 69 42.11 51.75 5.44 41.42 52.21 4.26 41.76 51.98 4.85 3 0 1000.00 -999 ? >GLU^A 70 43.39 47.72 9.69 43.14 46.76 11.51 43.26 47.24 10.60 2 0 1000.00 -999 ? >ASN^A 71 43.17 46.46 2.64 42.58 48.45 2.37 42.88 47.45 2.51 2 0 1000.00 -999 ? >ARG^A 72 42.10 40.77 7.10 42.56 42.18 9.80 42.33 41.47 8.45 2 0 1000.00 -999 ? >ILE^A 73 44.20 41.88 -0.80 44.58 42.93 -1.23 44.39 42.40 -1.02 3 0 1000.00 -999 ? >ASN.A 74 41.55 36.97 -2.31 41.74 35.03 -3.08 41.64 36.00 -2.70 2 0 1000.00 -999 ? >ILE~A 75 47.55 37.51 -2.81 48.21 37.57 -1.31 47.88 37.54 -2.06 3 0 1000.00 -999 ? >GLU~A 76 44.85 32.67 -3.87 46.93 32.33 -3.56 45.89 32.50 -3.71 2 0 1000.00 -999 ? >LEU~A 77 41.86 37.35 -7.26 40.03 36.15 -7.16 40.95 36.75 -7.21 3 0 1000.00 -999 ? >CYH~A 78 45.94 39.41 -8.37 44.68 40.91 -6.37 45.31 40.16 -7.37 2 0 1000.00 -999 ? >PRO.A 79 47.90 36.62 -10.83 48.58 38.04 -9.20 48.24 37.33 -10.02 3 0 1000.00 -999 ? >GLY~A 80 51.97 40.43 -8.28 51.36 40.40 -7.77 51.66 40.42 -8.02 1 0 1000.00 -999 ? >ILE~A 81 48.63 42.20 -5.17 47.80 42.59 -4.72 48.22 42.40 -4.94 3 0 1000.00 -999 ? >VAL~A 82 52.47 41.51 -2.88 52.66 39.33 -2.65 52.57 40.42 -2.77 3 0 1000.00 -999 ? >GLU~A 83 56.51 41.37 -5.89 56.18 40.14 -7.61 56.35 40.75 -6.75 2 0 1000.00 -999 ? >ILE~A 84 52.00 46.88 -5.42 52.04 47.33 -6.79 52.02 47.10 -6.11 3 0 1000.00 -999 ? >ASP.A 85 55.80 50.62 -3.10 56.01 52.11 -1.49 55.90 51.37 -2.30 2 0 1000.00 -999 ? >PHE.A 86 49.61 49.25 -2.35 48.87 47.03 -3.89 49.24 48.14 -3.12 3 0 1000.00 -999 ? >ASN.A 87 54.13 54.63 -0.06 53.76 54.38 2.04 53.95 54.51 0.99 2 0 1000.00 -999 ? >GLN.A 88 56.05 54.81 -4.61 56.33 56.85 -5.23 56.19 55.83 -4.92 2 0 1000.00 -999 ? >HIS.A 89 51.76 51.71 -7.62 53.50 51.77 -8.80 52.63 51.74 -8.21 3 0 1000.00 -999 ? >ASN~A 90 45.60 54.06 -6.23 46.24 56.08 -6.29 45.92 55.07 -6.26 2 0 1000.00 -999 ? >LEU~A 91 44.60 49.79 -2.59 44.54 47.88 -1.62 44.57 48.83 -2.10 3 0 1000.00 -999 ? >PHE~A 92 40.29 49.40 -4.11 39.74 48.43 -1.54 40.01 48.92 -2.83 3 0 1000.00 -999 ? >PRO~A 93 43.06 52.75 -8.63 42.97 50.50 -8.69 43.02 51.62 -8.66 3 0 1000.00 -999 ? >THR~A 94 46.09 48.10 -8.80 48.05 48.38 -7.85 47.07 48.24 -8.32 2 0 1000.00 -999 ? >ILE~A 95 42.72 45.28 -6.72 42.06 44.77 -5.48 42.39 45.03 -6.10 3 0 1000.00 -999 ? >GLU~A 96 39.81 47.25 -10.61 38.48 48.71 -11.36 39.14 47.98 -10.99 2 0 1000.00 -999 ? >SER~A 97 45.06 46.02 -13.31 45.98 48.31 -13.16 45.52 47.16 -13.23 2 0 1000.00 -999 ? >LEU~A 98 47.30 42.82 -10.50 49.28 43.58 -10.05 48.29 43.20 -10.27 3 0 1000.00 -999 ? >SER~A 99 43.44 40.88 -11.93 41.71 42.40 -11.12 42.57 41.64 -11.52 2 0 1000.00 -999 ? >LYS~A 100 42.25 42.30 -15.26 46.72 39.10 -17.02 44.49 40.70 -16.14 2 0 1000.00 -999 ? >ARG.A 101 38.69 38.85 -11.70 37.80 36.11 -12.79 38.24 37.48 -12.24 2 0 1000.00 -999 ? >LYS.A 102 36.55 45.24 -12.69 35.91 45.89 -18.93 36.23 45.57 -15.81 2 0 1000.00 -999 ? >ILE.A 103 35.84 46.73 -8.14 36.46 46.94 -6.90 36.15 46.83 -7.52 3 0 1000.00 -999 ? >ARG.A 104 31.10 43.35 -12.59 32.01 43.98 -15.44 31.56 43.66 -14.01 2 0 1000.00 -999 ? >TYR^A 105 28.42 43.63 -6.82 26.98 42.61 -8.99 27.70 43.12 -7.91 3 0 1000.00 -999 ? >SER^A 106 30.60 46.57 -2.42 31.87 48.35 -1.94 31.24 47.46 -2.18 2 0 1000.00 -999 ? >GLU^A 107 27.66 43.69 0.21 27.39 41.60 0.66 27.52 42.65 0.44 2 0 1000.00 -999 ? >SER^A 108 29.42 47.78 4.04 30.50 49.56 2.79 29.97 48.67 3.42 2 0 1000.00 -999 ? >ARG^A 109 27.84 44.04 9.37 27.66 41.56 11.19 27.75 42.80 10.28 2 0 1000.00 -999 ? >TYR^A 110 28.04 51.88 8.15 28.25 52.50 5.39 28.15 52.19 6.77 3 0 1000.00 -999 ? >ALA^A 111 29.05 49.84 13.83 29.56 48.42 14.10 29.30 49.13 13.97 2 0 1000.00 -999 ? >ALA^A 112 30.14 51.92 16.89 29.23 52.52 17.93 29.68 52.22 17.41 2 0 1000.00 -999 ? >ARG^A 113 33.46 47.70 17.24 35.92 45.85 16.76 34.69 46.78 17.00 2 0 1000.00 -999 ? >LEU^A 114 35.56 51.89 22.64 33.50 52.78 22.79 34.53 52.34 22.72 3 0 1000.00 -999 ? >ILE.A 115 39.71 53.86 18.64 40.28 54.03 18.07 39.99 53.94 18.35 3 0 1000.00 -999 ? >GLY~A 116 41.74 50.77 21.01 41.81 51.60 20.86 41.78 51.18 20.93 1 0 1000.00 -999 ? >ASN~A 117 45.99 51.09 23.31 47.83 52.03 23.83 46.91 51.56 23.57 2 0 1000.00 -999 ? >GLU~A 118 48.39 49.69 17.45 49.15 47.84 16.91 48.77 48.77 17.18 2 0 1000.00 -999 ? >ARG~A 119 41.99 49.87 15.63 41.67 46.83 15.54 41.83 48.35 15.59 2 0 1000.00 -999 ? >GLY~A 120 44.02 55.12 18.97 44.71 54.87 18.49 44.36 55.00 18.73 1 0 1000.00 -999 ? >PHE~A 122 48.18 53.88 13.97 50.17 51.91 14.04 49.17 52.90 14.00 3 0 1000.00 -999 ? >LEU~A 123 42.99 57.77 14.76 42.12 56.88 13.01 42.55 57.33 13.89 3 0 1000.00 -999 ? >ASP~A 124 45.18 61.81 17.57 46.88 62.21 16.47 46.03 62.01 17.02 2 0 1000.00 -999 ? >SER.A 126 41.84 58.89 23.09 39.61 59.41 23.92 40.73 59.15 23.51 2 0 1000.00 -999 ? >GLU.A 127 42.72 54.53 25.78 42.83 52.69 26.74 42.78 53.61 26.26 2 0 1000.00 -999 ? >ASP.A 128 38.64 54.02 26.03 38.02 52.14 26.79 38.33 53.08 26.41 2 0 1000.00 -999 ? >ALA.A 129 37.14 56.90 22.42 37.90 57.91 21.61 37.52 57.40 22.01 2 0 1000.00 -999 ? >PRO^A 130 32.89 57.40 21.45 34.61 56.11 20.76 33.75 56.75 21.11 3 0 1000.00 -999 ? >VAL^A 131 34.74 55.71 16.50 36.78 55.03 16.53 35.76 55.37 16.51 3 0 1000.00 -999 ? >LEU^A 132 32.06 55.54 12.41 30.46 54.95 11.01 31.26 55.25 11.71 3 0 1000.00 -999 ? >HIS^A 133 34.44 48.87 12.88 35.42 47.20 12.07 34.93 48.03 12.47 3 0 1000.00 -999 ? >LEU^A 134 33.20 51.00 7.87 32.80 51.45 5.93 33.00 51.23 6.90 3 0 1000.00 -999 ? >GLU^A 135 33.40 44.76 9.43 32.54 42.86 9.99 32.97 43.81 9.71 2 0 1000.00 -999 ? >GLN^A 136 33.62 47.96 1.72 34.39 49.72 0.87 34.01 48.84 1.29 2 0 1000.00 -999 ? >LEU^A 137 32.57 42.38 1.67 33.18 40.26 1.50 32.88 41.32 1.58 3 0 1000.00 -999 ? >VAL^A 138 34.57 44.35 -2.20 35.81 46.15 -2.57 35.19 45.25 -2.39 3 0 1000.00 -999 ? >PHE^A 139 31.67 41.08 -3.90 30.37 40.19 -1.57 31.02 40.64 -2.74 3 0 1000.00 -999 ? >PHE^A 140 37.13 40.98 -8.96 39.80 41.72 -8.58 38.46 41.35 -8.77 3 0 1000.00 -999 ? >SER^A 141 31.50 39.55 -9.30 32.05 37.18 -9.55 31.78 38.36 -9.42 2 0 1000.00 -999 ? >ARG.A 142 24.74 38.10 -7.28 23.36 40.86 -7.71 24.05 39.48 -7.50 2 0 1000.00 -999 ? >GLU.A 143 28.51 33.78 -7.43 27.76 34.21 -9.40 28.13 34.00 -8.42 2 0 1000.00 -999 ? >LEU^A 144 34.53 36.44 -7.61 36.34 35.58 -8.31 35.44 36.01 -7.96 3 0 1000.00 -999 ? >PRO^A 145 33.77 37.17 -1.96 34.72 38.17 -3.71 34.25 37.67 -2.83 3 0 1000.00 -999 ? >VAL^A 146 38.53 40.15 -3.58 39.39 41.67 -4.91 38.96 40.91 -4.24 3 0 1000.00 -999 ? >GLU^A 147 40.41 44.51 0.00 39.27 45.81 1.23 39.84 45.16 0.62 2 0 1000.00 -999 ? >PHE^A 148 36.74 41.49 5.08 35.29 41.77 7.42 36.01 41.63 6.25 3 0 1000.00 -999 ? >GLY^A 149 38.40 46.01 4.76 37.74 45.61 5.05 38.07 45.81 4.91 1 0 1000.00 -999 ? >ASN^A 150 38.57 46.58 9.44 37.58 44.75 9.81 38.08 45.67 9.62 2 0 1000.00 -999 ? >VAL^A 151 37.83 51.11 8.50 37.48 52.20 6.66 37.66 51.65 7.58 3 0 1000.00 -999 ? >TRP^A 152 40.29 50.71 11.75 38.27 48.97 14.60 39.28 49.84 13.17 3 0 1000.00 -999 ? >LEU^A 153 35.13 56.73 10.58 34.07 56.11 8.83 34.60 56.42 9.70 3 0 1000.00 -999 ? >LYS.A 154 36.90 59.04 13.78 41.83 62.58 14.69 39.36 60.81 14.24 2 0 1000.00 -999 ? >GLY.A 155 34.45 61.48 12.16 34.20 60.76 12.17 34.33 61.12 12.17 1 0 1000.00 -999 ? >ASN.A 156 30.34 61.95 10.60 28.45 61.53 11.45 29.40 61.74 11.02 2 0 1000.00 -999 ? >LYS.A 157 29.47 58.46 7.80 25.32 59.70 8.74 27.40 59.08 8.27 2 0 1000.00 -999 ? >LEU.A 160 25.79 57.14 2.79 25.42 55.58 4.06 25.60 56.36 3.42 3 0 1000.00 -999 ? >GLY.A 161 25.61 59.22 -1.16 26.07 58.55 -1.41 25.84 58.89 -1.28 1 0 1000.00 -999 ?