REMARK SOURCE hebe.pdb HEADER UNKNOWN FUNCTION 28-AUG-08 3EBT COMPND UNCHARACTERIZED NTF2-LIKE PROTEIN; COMPND 3 CHAIN: A; COMPND 4 ENGINEERED: YES SOURCE MOL_ID: 1; SOURCE 2 ORGANISM_SCIENTIFIC: BURKHOLDERIA PSEUDOMALLEI K96243; SOURCE 3 ORGANISM_COMMON: PSEUDOMONAS PSEUDOMALLEI; SOURCE 4 ORGANISM_TAXID: 272560; SOURCE 5 GENE: YP_110153.1, BPSS0132; SOURCE 6 EXPRESSION_SYSTEM: ESCHERICHIA COLI; SOURCE 7 EXPRESSION_SYSTEM_TAXID: 562; SOURCE 8 EXPRESSION_SYSTEM_STRAIN: HK100; SOURCE 9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID; SOURCE 10 EXPRESSION_SYSTEM_PLASMID: SPEEDET AUTHOR JOINT CENTER FOR STRUCTURAL GENOMICS,JOINT CENTER FOR STRUCTURAL AUTHOR 2 GENOMICS (JCSG) HET MSE A 1 8 HET MSE A 5 8 HET MSE A 39 8 HET MSE A 68 8 HET MSE A 125 8 HET UNL A 132 6 HET UNL A 133 6 SITE 1 AC1 8 SER A 11 TYR A 12 HIS A 35 LEU A 70 >MET?A 1 59.46 0.30 3.23 55.99 0.01 5.61 57.72 0.16 4.42 2 0 14.18 -999 ? O12 UNL A 133 >SER~A 2 58.89 3.83 1.92 56.90 4.06 0.58 57.89 3.95 1.25 2 0 15.28 -999 ? O12 UNL A 133 >ASN~A 3 58.06 8.01 4.33 57.47 8.96 6.14 57.77 8.49 5.23 2 0 15.78 -999 ? O12 UNL A 133 >ASN~A 4 54.32 7.15 0.57 54.88 9.16 0.16 54.60 8.15 0.36 2 0 14.43 -999 ? O12 UNL A 133 >MET?A 5 53.81 3.05 2.39 52.62 0.22 1.08 53.21 1.63 1.73 2 0 11.80 -999 ? O12 UNL A 133 >GLN~A 6 56.70 3.11 6.82 58.49 3.98 7.59 57.60 3.54 7.21 2 0 16.18 -999 ? O12 UNL A 133 >THR~A 7 51.71 6.33 6.09 51.89 8.41 5.60 51.80 7.37 5.85 2 0 10.35 -999 ? O12 UNL A 133 >VAL~A 8 49.15 4.29 4.13 48.74 3.77 2.04 48.94 4.03 3.08 3 0 6.95 -999 ? O12 UNL A 133 >ARG~A 9 52.36 -1.27 5.26 51.92 -3.88 3.60 52.14 -2.58 4.43 2 0 12.60 -999 ? O12 UNL A 133 >GLU~A 10 51.58 4.33 10.48 52.99 5.95 10.66 52.28 5.14 10.57 2 0 11.86 -999 ? O12 UNL A 133 >SER~A 11 46.01 5.12 8.12 46.64 6.77 6.48 46.33 5.94 7.30 2 0 -5.02 -999 ? O12 UNL A 133s >TYR~A 12 44.42 0.78 5.69 43.94 1.47 3.04 44.18 1.12 4.36 3 0 -3.71 -999 ? O12 UNL A 133s >GLU~A 13 47.87 0.04 11.57 47.39 -2.02 11.90 47.63 -0.99 11.73 2 0 10.18 -999 ? O12 UNL A 133 >ALA~A 14 43.96 4.50 12.58 44.38 5.89 12.04 44.17 5.20 12.31 2 0 7.00 -999 ? O12 UNL A 133 >PHE~A 15 39.25 2.67 9.02 36.68 1.86 8.27 37.97 2.26 8.64 3 0 4.49 -999 ? O10 UNL A 133 >HIS~A 16 41.19 -2.27 12.98 40.47 -4.22 13.25 40.83 -3.25 13.12 3 0 10.75 -999 ? O12 UNL A 133 >ARG~A 17 44.61 3.07 17.11 46.72 4.45 15.23 45.67 3.76 16.17 2 0 10.61 -999 ? O12 UNL A 133 >ASP~A 19 42.27 7.00 15.73 43.75 8.55 16.03 43.01 7.78 15.88 2 0 11.36 -999 ? O11 UNL A 133 >LEU~A 20 38.55 11.30 13.44 37.46 12.57 12.08 38.00 11.94 12.76 3 0 9.70 -999 ? O6 UNL A 132 >PRO~A 21 42.16 13.03 15.91 42.31 12.18 13.87 42.24 12.60 14.89 3 0 11.94 -999 ? O11 UNL A 133 >GLY~A 22 45.09 9.49 12.82 44.47 9.84 12.38 44.78 9.66 12.60 1 0 9.39 -999 ? O12 UNL A 133 >VAL~A 23 43.13 9.10 9.54 41.24 8.11 8.94 42.18 8.61 9.24 3 0 5.19 -999 ? O11 UNL A 133 >LEU~A 24 42.82 14.00 9.55 40.77 14.67 9.36 41.80 14.33 9.45 3 0 9.93 -999 ? O11 UNL A 133 >ALA~A 25 47.25 12.72 9.51 47.89 11.92 10.65 47.57 12.32 10.08 2 0 10.75 -999 ? O12 UNL A 133 >ALA.A 26 47.86 11.03 6.12 47.32 9.63 5.76 47.59 10.33 5.94 2 0 7.30 -999 ? O12 UNL A 133 >LEU^A 27 44.43 13.66 4.33 43.15 11.94 3.96 43.79 12.80 4.15 3 0 8.36 -999 ? O11 UNL A 133 >ALA^A 28 47.16 16.91 2.70 47.55 17.16 1.26 47.35 17.04 1.98 2 0 14.51 -999 ? O11 UNL A 133 >PRO^A 29 47.38 20.99 4.34 45.83 19.57 3.62 46.60 20.28 3.98 3 0 16.30 -999 ? O11 UNL A 133 >ASP^A 30 44.44 21.45 -0.75 46.40 21.02 -1.26 45.42 21.24 -1.00 2 0 18.20 -999 ? O11 UNL A 133 >VAL^A 31 43.08 16.72 -0.19 42.41 15.12 1.20 42.74 15.92 0.50 3 0 12.05 -999 ? O11 UNL A 133 >ARG^A 32 40.74 17.98 -5.87 41.86 20.89 -5.61 41.30 19.43 -5.74 2 0 18.38 -999 ? O7 UNL A 133 >TRP^A 33 40.13 11.05 1.75 36.69 9.99 3.24 38.41 10.52 2.49 3 0 5.91 -999 ? O7 UNL A 133 >THR^A 34 36.50 10.79 -3.48 36.69 11.44 -5.57 36.60 11.11 -4.53 2 0 9.91 -999 ? O7 UNL A 133 >HIS^A 35 33.92 7.76 -0.89 35.31 7.58 0.66 34.61 7.67 -0.11 3 0 -4.08 -999 ? O7 UNL A 133s >PRO~A 36 32.30 4.72 -5.47 31.97 6.94 -5.55 32.14 5.83 -5.51 3 0 7.41 -999 ? O1 UNL A 132 >ASP~A 37 28.88 9.89 -8.92 29.99 11.75 -8.82 29.43 10.82 -8.87 2 0 12.99 -999 ? O1 UNL A 132 >GLY~A 38 26.48 6.20 -7.33 26.84 6.60 -6.73 26.66 6.40 -7.03 1 0 9.78 -999 ? O1 UNL A 132 >MET?A 39 26.60 6.68 -3.53 27.23 3.56 -2.65 26.91 5.12 -3.09 2 0 4.99 -999 ? O1 UNL A 132 >SER~A 40 25.21 10.23 -3.72 26.68 11.35 -5.19 25.95 10.79 -4.46 2 0 10.71 -999 ? O1 UNL A 132 >PRO~A 41 21.49 7.96 -4.77 22.28 9.26 -3.11 21.89 8.61 -3.94 3 0 11.07 -999 ? O1 UNL A 132 >TYR~A 42 22.74 5.75 0.36 20.67 3.92 -0.22 21.71 4.83 0.07 3 0 8.72 -999 ? O1 UNL A 132 >GLY~A 43 25.25 11.32 1.02 25.52 10.57 0.84 25.38 10.95 0.93 1 0 9.19 -999 ? O1 UNL A 132 >LEU.A 44 28.20 7.79 1.19 28.05 7.51 3.33 28.12 7.65 2.26 3 0 4.65 -999 ? O1 UNL A 132 >GLY.A 45 29.41 10.07 -2.98 29.47 10.66 -2.43 29.44 10.36 -2.70 1 0 7.79 -999 ? O1 UNL A 132 >GLY^A 46 31.54 12.62 -4.71 31.87 12.15 -4.18 31.71 12.39 -4.45 1 0 10.91 -999 ? O1 UNL A 132 >THR^A 47 34.72 14.49 -3.94 36.27 14.90 -5.36 35.49 14.69 -4.65 2 0 12.37 -999 ? O7 UNL A 133 >LYS^A 48 35.31 16.02 -0.49 30.60 15.40 -0.29 32.95 15.71 -0.39 2 0 11.60 -999 ? O3 UNL A 132 >HIS.A 49 37.20 19.82 -2.83 36.65 19.38 -4.81 36.92 19.60 -3.82 3 0 15.79 -999 ? O7 UNL A 133 >GLY~A 50 39.91 19.47 2.62 39.42 18.86 2.57 39.67 19.17 2.59 1 0 14.56 -999 ? O11 UNL A 133 >HIS~A 51 42.30 17.82 6.99 44.27 17.11 6.83 43.28 17.47 6.91 3 0 12.59 -999 ? O11 UNL A 133 >ASP~A 52 36.26 21.13 7.32 37.26 22.15 8.88 36.76 21.64 8.10 2 0 16.70 -999 ? O6 UNL A 132 >GLU~A 53 34.23 19.92 2.55 34.17 20.44 0.52 34.20 20.18 1.54 2 0 15.49 -999 ? O6 UNL A 132 >VAL~A 54 36.34 14.70 4.94 38.25 13.75 4.45 37.29 14.23 4.69 3 0 8.71 -999 ? O6 UNL A 132 >ILE~A 55 35.78 16.03 9.62 36.39 16.57 10.75 36.09 16.30 10.19 3 0 11.35 -999 ? O6 UNL A 132 >ALA~A 56 31.72 15.77 7.64 31.25 17.00 6.84 31.48 16.38 7.24 2 0 11.76 -999 ? O6 UNL A 132 >PHE~A 57 32.32 11.12 3.51 32.04 8.74 2.06 32.18 9.93 2.78 3 0 5.63 -999 ? O5 UNL A 132 >ILE~A 58 34.45 10.53 8.69 35.78 9.98 8.96 35.12 10.25 8.83 3 0 5.31 -999 ? O6 UNL A 132 >ARG~A 59 31.75 14.76 12.76 33.74 16.67 14.20 32.75 15.71 13.48 2 0 13.73 -999 ? O6 UNL A 132 >HIS~A 60 26.13 11.38 6.17 24.18 11.08 5.46 25.15 11.23 5.82 3 0 9.77 -999 ? O3 UNL A 132 >VAL~A 61 28.72 7.27 7.56 30.54 6.26 6.89 29.63 6.76 7.23 3 0 5.23 -999 ? O3 UNL A 132 >PRO.A 62 28.71 7.09 11.97 27.40 6.33 10.29 28.06 6.71 11.13 3 0 8.79 -999 ? O6 UNL A 132 >THR.A 63 23.61 7.06 9.27 22.76 8.91 8.53 23.19 7.98 8.90 2 0 11.29 -999 ? O3 UNL A 132 >HIS.A 64 23.18 5.44 5.03 21.81 6.90 4.43 22.50 6.17 4.73 3 0 8.85 -999 ? O1 UNL A 132 >ILE^A 65 27.99 1.99 7.25 28.89 1.59 6.22 28.44 1.79 6.74 3 0 5.42 -999 ? O3 UNL A 132 >ALA^A 66 27.02 0.49 11.67 26.03 -0.53 12.25 26.52 -0.02 11.96 2 0 11.77 -999 ? O3 UNL A 132 >MET?A 68 33.14 -1.53 8.68 34.15 0.93 5.18 33.65 -0.30 6.93 2 0 3.90 -999 ? O4 UNL A 132 >LEU^A 70 39.62 -2.01 5.96 38.82 -0.21 5.12 39.22 -1.11 5.54 3 0 -4.92 -999 ? O8 UNL A 133s >ALA^A 71 41.07 -6.23 5.32 40.42 -7.60 5.41 40.74 -6.91 5.36 2 0 10.99 -999 ? O8 UNL A 133 >PRO^A 72 44.03 -3.17 4.39 43.26 -5.00 3.45 43.64 -4.09 3.92 3 0 8.52 -999 ? O12 UNL A 133 >ASP^A 73 44.63 -9.01 0.80 43.27 -8.82 -0.80 43.95 -8.92 0.00 2 0 13.57 -999 ? O8 UNL A 133 >GLU^A 74 47.26 -8.00 -3.19 48.83 -9.31 -2.52 48.05 -8.65 -2.86 2 0 16.97 -999 ? O12 UNL A 133 >PHE^A 75 49.49 -2.42 0.91 47.94 -2.43 3.24 48.71 -2.43 2.07 3 0 9.75 -999 ? O12 UNL A 133 >ILE^A 76 49.96 -2.09 -6.19 49.46 -2.91 -7.26 49.71 -2.50 -6.73 3 0 15.74 -999 ? O12 UNL A 133 >GLU^A 77 54.91 2.73 -3.63 55.99 2.81 -1.81 55.45 2.77 -2.72 2 0 15.47 -999 ? O12 UNL A 133 >SER^A 78 54.88 3.11 -7.94 55.21 3.20 -10.32 55.04 3.15 -9.13 2 0 20.38 -999 ? O12 UNL A 133 >GLY.A 79 56.43 6.53 -7.44 55.94 6.03 -6.90 56.19 6.28 -7.17 1 0 19.22 -999 ? O12 UNL A 133 >GLU.A 80 55.49 10.61 -4.77 55.77 12.69 -4.99 55.63 11.65 -4.88 2 0 18.98 -999 ? O12 UNL A 133 >ARG^A 81 49.25 7.39 -9.49 50.34 9.63 -11.33 49.79 8.51 -10.41 2 0 19.40 -999 ? O12 UNL A 133 >ILE^A 82 50.17 3.54 -2.39 51.02 3.11 -1.27 50.60 3.32 -1.83 3 0 11.02 -999 ? O12 UNL A 133 >VAL^A 83 46.85 1.26 -4.74 45.99 0.98 -6.71 46.42 1.12 -5.72 3 0 12.32 -999 ? O12 UNL A 133 >VAL^A 84 45.42 -0.91 -1.97 45.70 -0.40 0.13 45.56 -0.66 -0.92 3 0 8.65 -999 ? O12 UNL A 133 >LEU^A 85 43.78 -4.99 -3.79 44.33 -4.60 -5.89 44.06 -4.80 -4.84 3 0 12.89 -999 ? O8 UNL A 133 >GLY^A 86 41.15 -5.81 -0.25 40.61 -5.52 -0.78 40.88 -5.67 -0.51 1 0 9.94 -999 ? O8 UNL A 133 >THR^A 87 37.85 -6.84 1.23 38.01 -9.00 1.34 37.93 -7.92 1.28 2 0 11.82 -999 ? O8 UNL A 133 >ARG^A 88 34.89 -1.80 2.58 34.91 0.53 0.62 34.90 -0.63 1.60 2 0 -3.99 -999 ? O8 UNL A 133s >ARG^A 89 31.81 -9.20 7.27 29.58 -11.23 6.98 30.70 -10.21 7.13 2 0 15.63 -999 ? O2 UNL A 132 >VAL^A 90 30.22 -4.22 6.47 30.24 -2.65 4.91 30.23 -3.43 5.69 3 0 8.02 -999 ? O2 UNL A 132 >THR^A 91 27.20 -4.19 8.76 27.00 -5.26 10.61 27.10 -4.73 9.68 2 0 13.01 -999 ? O3 UNL A 132 >ALA^A 92 24.37 -1.82 7.74 23.41 -2.09 6.63 23.89 -1.96 7.19 2 0 10.38 -999 ? O1 UNL A 132 >VAL.A 93 22.18 0.15 10.15 21.88 2.26 10.74 22.03 1.21 10.45 3 0 12.40 -999 ? O3 UNL A 132 >ASN.A 94 18.85 -2.31 7.48 20.31 -3.15 6.14 19.58 -2.73 6.81 2 0 12.97 -999 ? O1 UNL A 132 >GLY.A 95 21.24 -5.07 10.65 21.36 -5.00 9.87 21.30 -5.03 10.26 1 0 15.62 -999 ? O1 UNL A 132 >ARG.A 96 19.74 -7.42 4.78 18.62 -4.76 3.68 19.18 -6.09 4.23 2 0 14.53 -999 ? O1 UNL A 132 >SER^A 97 26.04 -7.77 6.88 26.59 -7.75 9.24 26.32 -7.76 8.06 2 0 14.34 -999 ? O1 UNL A 132 >ALA^A 98 28.16 -8.74 3.89 27.44 -8.32 2.59 27.80 -8.53 3.24 2 0 12.33 -999 ? O1 UNL A 132 >THR^A 99 31.73 -8.99 2.69 33.29 -10.33 3.28 32.51 -9.66 2.99 2 0 13.56 -999 ? O8 UNL A 133 >LEU^A 100 31.76 -5.26 0.01 29.59 -4.98 -0.10 30.68 -5.12 -0.04 3 0 8.69 -999 ? O2 UNL A 132 >LYS^A 101 35.59 -5.59 -2.17 35.22 -9.19 -7.06 35.41 -7.39 -4.61 2 0 15.56 -999 ? O2 UNL A 132 >PHE^A 102 38.69 -1.82 -0.05 40.94 -2.37 1.49 39.81 -2.10 0.72 3 0 5.94 -999 ? O8 UNL A 133 >VAL^A 103 40.17 -0.58 -4.21 40.79 -1.11 -6.22 40.48 -0.85 -5.21 3 0 9.62 -999 ? O8 UNL A 133 >HIS^A 104 40.97 2.37 -0.33 39.48 2.08 1.11 40.23 2.22 0.39 3 0 -4.11 -999 ? O8 UNL A 133s >VAL^A 105 44.03 4.77 -4.74 43.98 5.76 -6.68 44.00 5.27 -5.71 3 0 10.43 -999 ? O7 UNL A 133 >TRP^A 106 45.86 4.53 -0.82 45.14 6.44 2.50 45.50 5.48 0.84 3 0 5.75 -999 ? O12 UNL A 133 >ARG^A 107 48.89 11.65 -8.05 47.29 14.34 -7.85 48.09 12.99 -7.95 2 0 17.12 -999 ? O7 UNL A 133 >PHE^A 108 51.43 9.82 -0.70 50.11 8.23 1.16 50.77 9.03 0.23 3 0 10.99 -999 ? O12 UNL A 133 >GLU^A 109 51.85 15.96 -4.63 50.89 17.57 -5.48 51.37 16.77 -5.05 2 0 19.27 -999 ? O12 UNL A 133 >ASN.A 110 55.50 17.03 0.47 57.39 17.99 0.53 56.44 17.51 0.50 2 0 20.94 -999 ? O12 UNL A 133 >GLY.A 111 54.37 12.51 2.02 53.86 13.01 1.80 54.12 12.76 1.91 1 0 14.96 -999 ? O12 UNL A 133 >ARG^A 112 50.48 16.03 5.30 52.61 16.26 7.56 51.54 16.14 6.43 2 0 15.90 -999 ? O12 UNL A 133 >ALA^A 113 47.87 12.30 0.22 46.86 11.19 0.62 47.36 11.75 0.42 2 0 9.62 -999 ? O12 UNL A 133 >VAL^A 114 46.60 14.99 -2.16 48.32 15.72 -3.31 47.46 15.36 -2.74 3 0 14.77 -999 ? O12 UNL A 133 >THR^A 115 44.05 12.54 -3.55 44.38 12.68 -5.72 44.21 12.61 -4.64 2 0 13.24 -999 ? O7 UNL A 133 >PHE^A 116 41.49 8.15 -0.16 40.24 5.82 0.68 40.87 6.99 0.26 3 0 -4.83 -999 ? O7 UNL A 133s >GLU^A 117 38.27 7.54 -6.11 37.95 8.42 -7.99 38.11 7.98 -7.05 2 0 10.52 -999 ? O7 UNL A 133 >ASP^A 118 36.78 4.45 -1.78 36.33 2.59 -0.94 36.56 3.52 -1.36 2 0 -3.63 -999 ? O7 UNL A 133s >HIS^A 119 37.94 3.33 -7.81 38.78 4.97 -8.78 38.36 4.15 -8.29 3 0 10.28 -999 ? O7 UNL A 133 >PHE.A 120 32.84 0.67 -3.40 31.42 2.74 -2.22 32.13 1.70 -2.81 3 0 4.78 -999 ? O2 UNL A 132 >ASP~A 121 30.49 -3.68 -6.51 28.82 -4.56 -7.46 29.66 -4.12 -6.99 2 0 12.08 -999 ? O1 UNL A 132 >THR~A 122 28.03 0.48 -5.62 28.54 2.16 -6.86 28.28 1.32 -6.24 2 0 8.48 -999 ? O1 UNL A 132 >ALA~A 123 24.98 -1.63 -6.59 24.73 -2.33 -7.91 24.86 -1.98 -7.25 2 0 12.29 -999 ? O1 UNL A 132 >GLU~A 124 27.05 -6.21 -5.37 25.67 -7.69 -4.82 26.36 -6.95 -5.09 2 0 13.66 -999 ? O1 UNL A 132 >MET?A 125 26.88 -1.63 -1.37 28.55 -0.41 1.39 27.72 -1.02 0.01 2 0 4.38 -999 ? O1 UNL A 132 >ILE~A 126 22.94 0.67 -3.14 22.53 1.83 -3.83 22.74 1.25 -3.48 3 0 9.23 -999 ? O1 UNL A 132 >ARG~A 127 22.07 -7.13 -3.32 21.40 -9.59 -4.94 21.74 -8.36 -4.13 2 0 17.09 -999 ? O1 UNL A 132 >LEU~A 128 24.86 -4.06 1.96 25.75 -4.97 3.75 25.30 -4.51 2.86 3 0 9.65 -999 ? O1 UNL A 132 >ILE~A 129 23.53 0.59 3.17 24.69 1.33 2.70 24.11 0.96 2.93 3 0 6.89 -999 ? O1 UNL A 132 >THR~A 130 19.05 0.05 1.63 17.93 -0.66 -0.11 18.49 -0.30 0.76 2 0 13.12 -999 ? O1 UNL A 132 >ALA.A 131 15.81 -0.90 3.59 14.44 -0.29 3.49 15.13 -0.59 3.54 2 0 16.43 -999 ? O1 UNL A 132