HEADER UNKNOWN FUNCTION 13-AUG-08 3E56 COMPND PUTATIVE UNCHARACTERIZED PROTEIN; COMPND 3 CHAIN: A; COMPND 4 FRAGMENT: UNP RESIDUES 1-88; COMPND 5 ENGINEERED: YES SOURCE MOL_ID: 1; SOURCE 2 ORGANISM_SCIENTIFIC: NOSTOC PUNCTIFORME; SOURCE 3 ORGANISM_TAXID: 63737; SOURCE 4 STRAIN: PCC 73102; SOURCE 5 GENE: NPUN_R1517; SOURCE 6 EXPRESSION_SYSTEM: ESCHERICHIA COLI; SOURCE 7 EXPRESSION_SYSTEM_TAXID: 562; SOURCE 8 EXPRESSION_SYSTEM_STRAIN: BL21(DE3); SOURCE 9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID; SOURCE 10 EXPRESSION_SYSTEM_PLASMID: PET28B AUTHOR M.A.KENNEDY,S.NI,M.J.SMOLA mLEU.A 13 23.15 51.76 13.02 23.48 51.63 13.96 22.03 50.69 10.97 21.98 50.54 9.75 22.65 51.96 11.60 23.73 52.62 10.71 3 0 1000.00-999 ?-999 ?-999 ? mGLU.A 14 21.60 49.79 11.87 21.95 50.00 12.78 19.58 48.97 10.66 18.76 49.85 10.93 20.74 48.65 11.63 20.15 48.26 12.99 2 0 1000.00-999 ?-999 ?-999 ? mVAL.A 15 19.54 48.23 9.55 20.31 47.61 9.47 17.69 47.02 8.53 18.19 45.97 8.95 18.48 48.32 8.55 19.03 48.65 7.13 3 0 1000.00-999 ?-999 ?-999 ? mGLY.A 16 16.44 47.09 8.08 16.08 48.02 7.97 14.97 46.01 6.47 14.96 47.09 5.87 15.68 45.89 7.79-999.99-999.99-999.99 1 0 1000.00-999 ?-999 ?-999 ? mVAL^A 17 14.36 44.92 6.03 14.53 44.09 6.57 12.12 44.87 5.40 11.77 43.99 6.20 13.52 44.91 4.86 13.72 43.67 3.99 3 0 1000.00-999 ?-999 ?-999 ? mTYR^A 18 11.30 45.85 4.99 11.70 46.48 4.33 8.96 45.85 4.36 9.14 46.47 3.32 9.95 45.97 5.49 9.75 47.34 6.15 3 0 1000.00-999 ?-999 ?-999 ? mGLU^A 19 7.94 45.05 4.58 8.03 44.49 5.40 5.73 45.94 4.27 5.39 45.86 5.45 6.78 45.00 3.70 6.24 43.60 3.62 2 0 1000.00-999 ?-999 ?-999 ? mCYH^A 20 5.25 46.83 3.41 5.40 46.58 2.46 3.34 48.20 2.99 3.29 47.77 1.81 4.58 48.05 3.83 5.47 49.27 3.59 2 0 1000.00-999 ?-999 ?-999 ? mGLU^A 21 2.33 48.79 3.60 2.50 49.09 4.53 0.51 50.32 3.38 0.45 50.59 4.58 1.07 48.98 2.94 0.13 47.86 3.37 2 0 1000.00-999 ?-999 ?-999 ? mILE^A 22 0.15 51.18 2.43 0.35 50.87 1.50 -1.81 52.52 2.02 -1.86 52.27 0.83 -0.47 52.45 2.75 0.42 53.65 2.33 3 0 1000.00-999 ?-999 ?-999 ? mHIS^A 23 -2.88 52.83 2.74 -2.70 52.93 3.72 -4.70 54.29 2.69 -4.94 54.39 3.90 -4.18 53.00 2.14 -5.12 51.88 2.55 3 0 1000.00-999 ?-999 ?-999 ? mLEU^A 24 -4.84 55.29 1.82 -4.62 55.04 0.88 -6.42 57.13 1.39 -6.35 57.05 0.16 -5.27 56.64 2.26 -4.14 57.62 2.10 3 0 1000.00-999 ?-999 ?-999 ? mLYS^A 25 -7.46 57.67 2.02 -7.47 57.53 3.01 -8.53 59.79 1.81 -8.48 59.99 3.02 -8.47 58.40 1.27 -9.85 57.75 1.38 2 0 1000.00-999 ?-999 ?-999 ? mPHE^A 26 -8.60 60.78 0.93 -8.77 60.50 -0.01 -9.03 63.12 0.34 -9.17 62.74 -0.83 -8.44 62.17 1.37 -6.95 62.51 1.70 3 0 1000.00-999 ?-999 ?-999 ? mARG^A 27 -9.40 64.31 0.76 -9.35 64.42 1.76 -8.74 66.39 -0.19 -8.13 66.64 0.84 -9.82 65.37 -0.17 -11.15 66.05 0.25 2 0 1000.00-999 ?-999 ?-999 ? mLEU^A 28 -8.52 66.99 -1.37 -9.20 66.77 -2.07 -7.86 69.01 -2.48 -8.44 68.75 -3.52 -7.40 67.89 -1.59 -6.29 67.17 -2.37 3 0 1000.00-999 ?-999 ?-999 ? mILE.A 29 -7.51 70.24 -2.12 -7.23 70.31 -1.16 -6.22 71.37 -3.83 -5.16 71.42 -3.20 -7.53 71.36 -3.06 -7.71 72.74 -2.33 3 0 1000.00-999 ?-999 ?-999 ? mGLU.A 30 -6.26 71.33 -5.17 -7.17 71.22 -5.57 -5.28 72.16 -7.25 -6.42 72.18 -7.77 -5.03 71.42 -5.95 -4.45 70.00 -6.20 2 0 1000.00-999 ?-999 ?-999 ? mGLU~A 31 -4.22 72.76 -7.80 -3.42 72.82 -7.20 -4.53 72.18 -10.19 -3.82 71.17 -10.25 -4.26 73.27 -9.16 -2.93 73.93 -9.50 2 0 1000.00-999 ?-999 ?-999 ? mLYS~A 32 -5.51 72.43 -11.05 -5.97 73.30 -10.92 -4.66 71.04 -12.98 -4.52 69.87 -13.34 -5.84 71.46 -12.10 -6.99 71.98 -12.97 2 0 1000.00-999 ?-999 ?-999 ? mSER~A 33 -3.79 72.00 -13.32 -3.99 72.89 -12.91 -1.63 70.72 -13.66 -0.83 70.14 -14.43 -2.66 71.71 -14.23 -1.98 73.02 -14.62 2 0 1000.00-999 ?-999 ?-999 ? mLEU~A 34 -1.67 70.51 -12.33 -2.39 71.01 -11.85 -1.23 68.17 -11.59 -0.50 67.27 -11.20 -0.76 69.62 -11.64 -0.52 70.14 -10.22 3 0 1000.00-999 ?-999 ?-999 ? mLEU~A 35 -2.47 67.93 -12.00 -2.94 68.73 -12.40 -3.30 65.97 -13.21 -3.88 64.88 -13.26 -3.05 66.61 -11.88 -4.37 66.71 -11.14 3 0 1000.00-999 ?-999 ?-999 ? mSER.A 36 -2.87 66.61 -14.28 -2.34 67.44 -14.11 -2.08 65.16 -16.24 -2.25 64.70 -17.36 -3.16 66.08 -15.59 -3.53 67.24 -16.49 2 0 1000.00-999 ?-999 ?-999 ? mASP~A 37 -0.99 64.90 -15.54 -1.03 65.26 -14.60 0.20 62.85 -15.30 0.43 62.83 -14.12 0.16 64.17 -16.06 1.42 65.04 -15.84 2 0 1000.00-999 ?-999 ?-999 ? mARG~A 38 -0.04 61.73 -15.97 -0.15 61.87 -16.95 1.13 60.12 -14.50 1.04 59.59 -13.39 -0.14 60.44 -15.30 -0.42 59.30 -16.33 2 0 1000.00-999 ?-999 ?-999 ? mGLU~A 39 2.28 60.45 -15.06 2.16 60.95 -15.91 3.95 60.94 -13.24 4.74 60.49 -12.42 3.59 60.12 -14.47 4.69 60.30 -15.52 2 0 1000.00-999 ?-999 ?-999 ? mGLN~A 40 3.36 62.13 -13.10 2.75 62.36 -13.86 2.46 63.04 -10.94 2.63 63.66 -9.89 3.60 63.00 -11.95 3.91 64.42 -12.42 2 0 1000.00-999 ?-999 ?-999 ? mLEU~A 41 1.33 62.38 -11.23 1.36 61.84 -12.07 0.46 62.00 -8.95 0.13 62.69 -8.00 0.16 62.46 -10.37 -1.01 61.63 -10.93 3 0 1000.00-999 ?-999 ?-999 ? mLEU~A 42 1.05 60.81 -8.80 1.29 60.33 -9.64 2.19 61.26 -6.73 1.94 61.52 -5.57 1.29 60.32 -7.46 1.90 58.93 -7.49 3 0 1000.00-999 ?-999 ?-999 ? mGLN~A 43 3.25 61.74 -7.36 3.36 61.48 -8.32 3.49 63.87 -6.18 3.76 64.38 -5.07 4.17 62.60 -6.65 5.37 62.96 -7.51 2 0 1000.00-999 ?-999 ?-999 ? mVAL~A 44 2.60 64.40 -7.00 2.49 63.90 -7.86 1.01 65.38 -5.35 0.97 66.20 -4.46 1.85 65.64 -6.61 0.97 66.15 -7.80 3 0 1000.00-999 ?-999 ?-999 ? mLEU~A 45 0.40 64.21 -5.29 0.52 63.65 -6.11 0.50 63.53 -2.90 0.14 63.99 -1.83 -0.38 63.79 -4.13 -1.27 62.56 -4.46 3 0 1000.00-999 ?-999 ?-999 ? mLEU~A 46 1.64 62.85 -3.08 1.79 62.51 -4.01 3.06 63.98 -1.42 3.19 64.12 -0.23 2.59 62.63 -1.97 3.79 61.78 -2.41 3 0 1000.00-999 ?-999 ?-999 ? mASP~A 47 3.28 64.97 -2.29 3.06 64.73 -3.23 2.76 66.97 -0.96 3.12 67.58 0.04 3.78 66.28 -1.85 4.11 67.17 -3.06 2 0 1000.00-999 ?-999 ?-999 ? mALA~A 48 1.47 66.88 -1.33 1.33 66.38 -2.19 0.24 66.73 0.79 0.07 67.35 1.83 0.37 67.46 -0.53 -0.94 67.38 -1.29 2 0 1000.00-999 ?-999 ?-999 ? mLEU~A 49 0.32 65.39 0.76 0.37 65.03 -0.17 1.46 64.93 2.92 1.29 65.01 4.14 0.32 64.56 1.99 0.38 63.07 1.64 3 0 1000.00-999 ?-999 ?-999 ? mTHR~A 50 2.62 65.19 2.36 2.60 65.08 1.36 3.55 66.99 3.73 3.83 67.21 4.91 3.79 65.60 3.13 5.09 65.56 2.26 2 0 1000.00-999 ?-999 ?-999 ? mGLU~A 51 3.04 67.93 2.94 2.88 67.65 2.00 1.77 69.14 4.61 1.95 69.78 5.64 2.76 69.27 3.47 2.19 70.15 2.37 2 0 1000.00-999 ?-999 ?-999 ? mGLY~A 52 0.74 68.33 4.44 0.74 67.88 3.55 -0.98 69.42 5.99 -1.22 69.56 7.18 -0.29 68.14 5.49-999.99-999.99-999.99 1 0 1000.00-999 ?-999 ?-999 ? mSER.A 53 -1.28 70.35 5.10 -0.99 70.12 4.16 -3.51 71.41 5.56 -4.15 71.02 4.59 -1.99 71.58 5.49 -1.63 72.72 4.54 2 0 1000.00-999 ?-999 ?-999 ? mASP.A 54 -4.07 71.70 6.71 -3.41 71.96 7.42 -6.31 72.68 6.09 -7.53 72.58 5.96 -5.50 71.65 6.93 -5.82 71.87 8.43 2 0 1000.00-999 ?-999 ?-999 ? mASP.A 55 -5.64 73.67 5.53 -4.69 73.75 5.83 -6.43 73.87 3.19 -7.27 74.29 2.38 -6.28 74.55 4.55 -5.45 75.81 4.36 2 0 1000.00-999 ?-999 ?-999 ? mPHE.A 56 -5.63 72.85 2.90 -5.00 72.57 3.64 -6.13 70.75 1.59 -6.65 70.26 0.60 -5.71 72.21 1.57 -4.38 72.32 0.85 3 0 1000.00-999 ?-999 ?-999 ? mLEU^A 57 -5.93 70.07 2.71 -5.70 70.64 3.50 -6.89 68.15 3.92 -6.74 68.65 5.03 -6.05 68.62 2.76 -4.65 68.00 2.89 3 0 1000.00-999 ?-999 ?-999 ? mGLU^A 58 -7.78 67.21 3.66 -7.89 67.01 2.69 -8.33 65.02 4.59 -8.78 64.43 3.61 -8.51 66.54 4.70 -10.02 66.84 4.62 2 0 1000.00-999 ?-999 ?-999 ? mTHR^A 59 -7.70 64.39 5.58 -7.36 64.97 6.31 -8.80 62.22 6.01 -9.22 62.44 7.12 -7.56 62.93 5.52 -6.36 62.46 6.29 2 0 1000.00-999 ?-999 ?-999 ? mLEU^A 60 -9.39 61.39 5.17 -8.90 61.23 4.31 -10.47 59.40 6.12 -11.11 59.07 7.11 -10.67 60.78 5.50 -11.58 60.70 4.27 3 0 1000.00-999 ?-999 ?-999 ? mGLN^A 61 -9.60 58.59 5.53 -9.17 58.98 4.71 -7.80 57.06 5.82 -7.26 57.46 4.79 -9.29 57.26 6.03 -10.07 56.16 5.27 2 0 1000.00-999 ?-999 ?-999 ? mALA^A 62 -7.14 56.43 6.80 -7.73 56.18 7.57 -5.39 54.80 7.36 -5.81 54.52 8.48 -5.69 56.15 6.75 -4.94 57.19 7.48 2 0 1000.00-999 ?-999 ?-999 ? mSER^A 63 -4.65 53.97 6.64 -4.46 54.27 5.70 -2.74 52.62 6.76 -2.45 52.69 5.57 -4.19 52.72 7.19 -5.00 51.56 6.66 2 0 1000.00-999 ?-999 ?-999 ? mVAL^A 64 -1.83 52.53 7.74 -2.23 52.50 8.66 0.21 51.27 8.21 0.11 51.17 9.44 -0.39 52.47 7.49 0.29 53.78 7.97 3 0 1000.00-999 ?-999 ?-999 ? mLYS^A 65 0.75 50.33 7.44 0.73 50.54 6.46 2.77 48.97 7.55 3.07 49.30 6.40 1.33 49.10 8.01 0.53 47.90 7.55 2 0 1000.00-999 ?-999 ?-999 ? mALA^A 66 3.67 48.55 8.44 3.30 48.44 9.37 5.57 47.11 8.98 5.65 47.22 10.20 5.07 48.28 8.11 5.93 49.53 8.28 2 0 1000.00-999 ?-999 ?-999 ? mGLN^A 67 5.88 45.98 8.35 5.80 46.04 7.35 7.62 44.28 8.53 7.80 44.15 7.32 6.30 44.78 9.08 5.21 43.71 8.92 2 0 1000.00-999 ?-999 ?-999 ? mGLU^A 68 8.57 44.03 9.42 8.33 44.24 10.37 9.66 42.07 8.48 8.88 41.30 9.03 9.85 43.49 9.00 10.88 43.51 10.13 2 0 1000.00-999 ?-999 ?-999 ? mVAL^A 69 10.33 41.79 7.36 10.87 42.56 7.01 11.71 40.04 6.45 12.68 40.84 6.56 10.28 40.49 6.71 9.44 40.46 5.39 3 0 1000.00-999 ?-999 ?-999 ? mSER~A 70 11.84 38.76 6.14 11.00 38.22 6.12 13.64 38.82 4.55 12.88 39.10 3.64 13.13 38.21 5.85 13.03 36.69 5.78 2 0 1000.00-999 ?-999 ?-999 ? mGLU~A 71 14.94 39.04 4.47 15.46 38.73 5.26 15.46 38.91 2.02 15.67 39.48 0.96 15.52 39.69 3.32 16.95 40.00 3.64 2 0 1000.00-999 ?-999 ?-999 ? mPHE~A 72 15.19 37.61 2.11 15.10 37.30 3.06 13.88 37.18 0.07 13.87 36.91 -1.12 15.02 36.72 0.95 14.77 35.26 1.40 3 0 1000.00-999 ?-999 ?-999 ? mLYS~A 73 12.92 37.88 0.69 13.05 37.96 1.67 12.15 39.73 -0.87 11.42 40.07 -1.82 11.78 38.49 -0.03 10.65 38.81 0.95 2 0 1000.00-999 ?-999 ?-999 ? mALA~A 74 13.25 40.40 -0.55 13.72 40.04 0.26 14.11 41.22 -2.82 14.50 40.10 -3.13 13.71 41.54 -1.35 14.85 42.24 -0.62 2 0 1000.00-999 ?-999 ?-999 ? mSER~A 75 13.98 42.21 -3.72 13.52 43.02 -3.35 16.01 41.92 -5.15 16.69 42.32 -4.19 14.48 42.11 -5.11 14.09 43.36 -5.89 2 0 1000.00-999 ?-999 ?-999 ? mPRO~A 76 16.55 41.29 -6.23 15.89 40.91 -6.88 18.73 42.52 -6.13 19.70 42.55 -5.36 18.02 41.19 -6.39 18.18 40.77 -7.86 3 0 1000.00-999 ?-999 ?-999 ? mGLN~A 77 18.25 43.61 -6.73 17.45 43.44 -7.31 18.86 45.35 -5.06 19.84 45.88 -4.57 18.85 44.93 -6.55 18.09 45.94 -7.38 2 0 1000.00-999 ?-999 ?-999 ? mMET~A 78 17.74 45.12 -4.37 16.99 44.77 -4.93 18.51 44.44 -2.07 19.12 44.90 -1.10 17.65 45.37 -2.93 16.17 45.36 -2.48 2 0 1000.00-999 ?-999 ?-999 ? mARG~A 79 18.60 43.16 -2.41 18.03 42.89 -3.18 20.96 42.66 -1.79 21.72 42.54 -0.82 19.49 42.26 -1.66 19.31 40.80 -2.06 2 0 1000.00-999 ?-999 ?-999 ? mARG~A 80 21.36 43.12 -2.98 20.66 43.05 -3.69 22.92 44.91 -2.34 23.95 45.04 -1.70 22.72 43.68 -3.20 22.99 44.02 -4.68 2 0 1000.00-999 ?-999 ?-999 ? mGLN~A 81 21.91 45.79 -2.28 21.10 45.54 -2.81 22.17 46.67 0.00 22.95 47.31 0.69 22.01 47.00 -1.47 20.80 47.94 -1.69 2 0 1000.00-999 ?-999 ?-999 ? mLEU~A 82 21.46 45.65 0.46 20.84 45.24 -0.21 22.99 44.71 2.18 23.50 45.03 3.25 21.57 45.18 1.82 20.56 44.06 2.01 3 0 1000.00-999 ?-999 ?-999 ? mMET~A 83 23.64 43.96 1.29 23.09 43.72 0.49 25.97 44.75 1.57 26.86 44.83 2.41 25.04 43.53 1.50 25.48 42.58 0.39 2 0 1000.00-999 ?-999 ?-999 ? mARG~A 84 25.73 45.71 0.69 24.95 45.51 0.10 26.41 47.68 1.90 27.43 48.11 2.41 26.50 46.92 0.57 25.90 47.71 -0.58 2 0 1000.00-999 ?-999 ?-999 ? mLEU~A 85 25.21 47.78 2.48 24.47 47.34 1.98 25.56 47.73 4.95 26.03 48.33 5.89 25.01 48.48 3.76 23.52 48.78 3.99 3 0 1000.00-999 ?-999 ?-999 ? mARG~A 86 25.46 46.41 4.96 25.08 46.00 4.14 27.43 45.47 6.16 28.01 45.12 7.20 25.91 45.67 6.13 25.09 44.39 6.26 2 0 1000.00-999 ?-999 ?-999 ? mASN~A 87 28.09 45.78 5.05 27.50 46.12 4.32 30.24 46.93 4.56 30.20 47.92 5.31 29.54 45.63 4.93 29.87 44.53 3.90 2 0 1000.00-999 ?-999 ?-999 ?