HEADER UNKNOWN FUNCTION 20-JAN-08 3C0F COMPND UNCHARACTERIZED PROTEIN AF_1514; COMPND 3 CHAIN: B; COMPND 4 ENGINEERED: YES SOURCE MOL_ID: 1; SOURCE 2 ORGANISM_SCIENTIFIC: ARCHAEOGLOBUS FULGIDUS DSM 4304; SOURCE 3 ORGANISM_TAXID: 224325; SOURCE 4 STRAIN: DSM4304; SOURCE 5 GENE: AF1514; SOURCE 6 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21; SOURCE 7 EXPRESSION_SYSTEM_TAXID: 511693; SOURCE 8 EXPRESSION_SYSTEM_STRAIN: BL21; SOURCE 9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID; SOURCE 10 EXPRESSION_SYSTEM_PLASMID: PET28B AUTHOR Y.LI,P.BAHTI,N.SHAW,G.SONG,J.YIN,J.-Y.ZHU,H.ZHANG,H.XU,B.-C.WANG,Z.- AUTHOR 2 J.LIU HET MLY B 8 11 HET MLY B 25 11 HET MLY B 30 11 HET MLY B 43 11 HET MLY B 71 11 mILE.B 3 20.36 31.98 -13.44 20.52 32.09 -14.42 18.92 30.94 -11.74 17.82 31.20 -12.26 20.13 31.82 -12.01 19.97 33.18 -11.34 3 0 8.01-999 ?-999 ?-999 ? CH1 MLY B 71 mMET.B 4 19.12 29.89 -10.96 20.07 29.82 -10.64 18.17 28.61 -9.11 19.13 29.00 -8.45 18.08 28.93 -10.60 18.22 27.65 -11.44 2 0 6.01-999 ?-999 ?-999 ? N MLY B 71 mASP^B 5 17.17 27.89 -8.59 16.40 27.75 -9.21 18.44 26.44 -7.07 18.77 25.70 -8.00 17.24 27.38 -7.22 15.95 26.64 -6.85 2 0 5.34-999 ?-999 ?-999 ? N MLY B 71 mGLU^B 6 19.10 26.51 -5.92 18.67 27.10 -5.24 20.33 25.02 -4.36 19.82 25.52 -3.36 20.34 25.76 -5.69 21.55 26.71 -5.73 2 0 3.32-999 ?-999 ?-999 ? O MLY B 71 mILE^B 7 20.89 23.82 -4.37 21.16 23.50 -5.28 22.55 22.58 -3.14 23.01 21.93 -4.09 21.10 23.04 -3.15 20.11 21.83 -3.06 3 0 2.43-999 ?-999 ?-999 ? N MLY B 8 mVAL^B 9 25.36 20.48 -1.11 25.20 21.17 -1.81 27.11 19.31 0.16 27.88 19.11 -0.79 25.61 19.46 -0.09 24.99 18.10 -0.48 3 0 2.45-999 ?-999 ?-999 ? C MLY B 8 mASN.B 10 27.50 19.42 1.42 26.76 19.64 2.05 28.98 18.09 2.85 28.61 18.24 4.02 28.89 19.23 1.85 29.45 20.53 2.44 2 0 5.31-999 ?-999 ?-999 ? O MLY B 8 mLEU.B 11 29.46 16.94 2.38 29.72 16.98 1.41 31.00 15.54 3.66 31.95 15.80 2.91 29.57 15.74 3.20 29.08 14.50 2.44 3 0 8.51-999 ?-999 ?-999 ? O MLY B 8 mGLN.B 12 31.15 15.08 4.90 30.29 14.94 5.39 33.21 13.69 4.75 34.43 13.75 4.59 32.47 14.81 5.48 32.34 14.47 6.97 2 0 11.01-999 ?-999 ?-999 ? CH1 MLY B 43 mLYS.B 13 32.47 12.69 4.29 31.49 12.77 4.49 33.31 11.92 2.09 32.68 12.84 1.54 33.05 11.57 3.56 32.17 10.32 3.67 2 0 9.58-999 ?-999 ?-999 ? CH1 MLY B 43 mGLU.B 14 34.23 11.19 1.48 34.72 10.56 2.08 33.43 10.53 -0.73 33.02 9.44 -0.31 34.48 11.31 0.05 35.89 10.82 -0.31 2 0 6.47-999 ?-999 ?-999 ? CH1 MLY B 43 mVAL.B 15 32.99 11.09 -1.86 33.42 11.97 -2.07 32.47 10.22 -4.12 33.24 11.02 -4.67 31.98 10.45 -2.69 30.64 11.26 -2.72 3 0 8.04-999 ?-999 ?-999 ? CH1 MLY B 43 mSER~B 16 32.02 9.11 -4.70 31.52 8.50 -4.08 31.18 9.53 -6.94 30.22 10.07 -6.39 32.24 8.82 -6.11 32.16 7.31 -6.35 2 0 9.14-999 ?-999 ?-999 ? CH1 MLY B 43 mLEU~B 17 31.35 9.52 -8.26 32.20 9.09 -8.57 28.99 9.45 -8.98 27.98 10.14 -8.89 30.36 10.11 -9.16 30.83 10.03 -10.62 3 0 10.44-999 ?-999 ?-999 ? CB MLY B 43 mGLU~B 18 28.97 8.11 -8.93 29.88 7.70 -9.02 27.04 7.68 -7.44 25.83 7.90 -7.41 27.74 7.34 -8.76 28.02 5.84 -8.86 2 0 8.90-999 ?-999 ?-999 ? CD MLY B 25 mGLU~B 19 27.82 7.70 -6.36 28.78 7.48 -6.54 26.72 9.45 -4.99 25.63 9.66 -4.44 27.30 8.04 -5.03 28.39 7.87 -3.97 2 0 9.04-999 ?-999 ?-999 ? N MLY B 25 mALA~B 20 27.45 10.41 -5.56 28.31 10.09 -5.95 25.74 11.98 -6.42 24.85 12.74 -6.03 27.02 11.81 -5.60 28.13 12.70 -6.14 2 0 7.30-999 ?-999 ?-999 ? N MLY B 25 mGLU~B 21 25.66 11.28 -7.55 26.47 10.73 -7.75 23.25 10.77 -7.70 22.17 11.35 -7.78 24.48 11.32 -8.41 24.71 10.60 -9.74 2 0 5.01-999 ?-999 ?-999 ? N MLY B 25 mARG~B 22 23.43 9.65 -6.99 24.35 9.27 -7.06 21.85 10.02 -5.12 20.64 10.20 -4.97 22.36 9.05 -6.20 22.82 7.72 -5.58 2 0 4.59-999 ?-999 ?-999 ? CB MLY B 25 mTYR~B 23 22.78 10.65 -4.40 23.72 10.36 -4.61 21.65 12.84 -4.02 20.63 13.27 -3.45 22.46 11.69 -3.41 23.73 12.26 -2.77 3 0 4.55-999 ?-999 ?-999 ? N MLY B 25 mALA~B 24 22.11 13.33 -5.17 22.94 12.88 -5.49 20.06 14.06 -6.39 19.11 14.83 -6.20 21.46 14.43 -5.89 22.33 14.90 -7.05 2 0 2.44-999 ?-999 ?-999 ? N MLY B 25 mASN~B 26 18.15 11.71 -5.26 18.73 11.83 -6.06 16.72 12.84 -3.56 15.53 12.90 -3.24 17.30 11.53 -4.08 18.04 10.77 -2.99 2 0 2.44-999 ?-999 ?-999 ? C MLY B 25 mILE~B 27 17.55 13.88 -3.50 18.49 13.65 -3.73 16.11 15.80 -4.13 15.04 16.27 -3.75 17.09 15.22 -3.11 18.28 16.18 -2.89 3 0 4.45-999 ?-999 ?-999 ? O MLY B 25 mALA~B 28 16.49 15.75 -5.41 17.39 15.35 -5.55 14.23 15.66 -6.49 13.25 16.35 -6.78 15.64 16.25 -6.50 16.31 16.00 -7.85 2 0 3.87-999 ?-999 ?-999 ? O MLY B 25 mSER~B 29 14.14 14.38 -6.12 15.01 13.99 -5.80 11.77 14.14 -5.28 10.60 13.85 -5.51 12.89 13.61 -6.19 13.15 12.13 -5.92 2 0 2.45-999 ?-999 ?-999 ? N MLY B 30 mTYR.B 31 10.88 17.14 -5.18 11.27 16.34 -4.72 9.56 17.99 -7.14 8.80 18.81 -7.66 10.29 18.31 -5.84 11.37 19.38 -6.04 3 0 2.45-999 ?-999 ?-999 ? C MLY B 30 mGLY.B 32 9.76 16.77 -7.63 10.40 16.21 -7.10 9.96 15.34 -9.61 10.85 14.71 -9.03 9.10 16.32 -8.85-999.99-999.99-999.99 1 0 4.97-999 ?-999 ?-999 ? C MLY B 30 mASP^B 33 9.69 15.22 -10.90 8.94 15.81 -11.21 11.73 14.96 -12.22 11.74 15.89 -13.04 10.41 14.32 -11.79 9.54 13.99 -13.00 2 0 7.75-999 ?-999 ?-999 ? N MLY B 30 mGLY^B 34 12.84 14.46 -11.67 12.67 13.66 -11.09 14.82 14.53 -13.17 15.32 13.41 -13.22 14.16 15.03 -11.90-999.99-999.99-999.99 1 0 6.35-999 ?-999 ?-999 ? O MLY B 25 mILE^B 35 14.82 15.38 -14.20 14.31 16.22 -14.04 16.93 15.62 -15.36 17.15 16.83 -15.44 15.51 15.08 -15.45 14.78 15.71 -16.68 3 0 6.88-999 ?-999 ?-999 ? CH2 MLY B 25 mLEU^B 36 17.90 14.72 -15.19 17.58 13.77 -15.12 19.83 15.52 -16.47 19.85 14.71 -17.40 19.30 15.13 -15.10 20.17 14.02 -14.48 3 0 5.47-999 ?-999 ?-999 ? CH1 MLY B 25 mLEU^B 37 20.26 16.77 -16.59 20.25 17.29 -15.73 22.23 17.29 -18.03 22.73 16.93 -19.09 20.71 17.33 -17.86 20.17 18.75 -18.05 3 0 8.42-999 ?-999 ?-999 ? CH1 MLY B 25 mSER^B 38 22.95 17.66 -16.98 22.42 17.91 -16.17 25.07 17.50 -15.67 24.44 17.72 -14.63 24.41 17.69 -17.03 24.91 18.99 -17.68 2 0 9.51-999 ?-999 ?-999 ? CH1 MLY B 25 mVAL^B 39 26.33 17.08 -15.69 26.67 16.88 -16.61 28.53 17.47 -14.73 29.04 17.41 -15.85 27.13 16.92 -14.48 27.20 15.43 -14.01 3 0 9.30-999 ?-999 ?-999 ? CG MLY B 71 mHIS^B 40 29.13 18.02 -13.68 28.55 18.08 -12.87 31.23 18.09 -12.44 30.68 18.18 -11.34 30.51 18.49 -13.72 30.55 20.00 -13.90 3 0 7.68-999 ?-999 ?-999 ? N MLY B 43 mASP^B 41 32.47 17.63 -12.60 32.73 17.51 -13.56 34.37 18.45 -11.41 35.21 18.59 -12.30 33.35 17.31 -11.48 34.03 15.96 -11.72 2 0 4.33-999 ?-999 ?-999 ? N MLY B 43 mSER.B 42 34.28 19.25 -10.35 33.56 19.00 -9.71 36.62 20.03 -9.78 37.53 20.86 -9.86 35.19 20.40 -10.17 34.59 21.41 -9.18 2 0 2.44-999 ?-999 ?-999 ? N MLY B 43 mTHR.B 44 38.23 17.15 -11.17 37.79 17.57 -10.37 38.64 17.31 -13.64 39.40 17.17 -14.60 38.88 16.55 -12.34 38.41 15.09 -12.57 2 0 2.46-999 ?-999 ?-999 ? C MLY B 43 mGLY.B 45 37.57 18.11 -13.67 37.08 18.16 -12.80 36.22 18.05 -15.76 35.76 18.57 -16.78 37.18 18.83 -14.87-999.99-999.99-999.99 1 0 5.06-999 ?-999 ?-999 ? C MLY B 43 mTYR.B 46 35.92 16.81 -15.37 36.38 16.52 -14.53 33.60 16.60 -16.23 33.11 17.22 -15.29 34.98 15.97 -16.12 34.85 14.59 -15.47 3 0 7.31-999 ?-999 ?-999 ? C MLY B 43 mARG^B 47 32.99 16.45 -17.40 33.56 16.08 -18.13 30.84 15.84 -18.48 31.45 15.19 -19.34 31.58 16.81 -17.57 31.42 18.26 -18.05 2 0 9.94-999 ?-999 ?-999 ? N MLY B 43 mALA^B 48 29.54 15.74 -18.26 29.24 16.19 -17.42 27.43 15.95 -19.37 26.95 16.57 -18.42 28.63 15.03 -19.16 28.21 13.70 -18.56 2 0 11.28-999 ?-999 ?-999 ? CH1 MLY B 25 mPRO.B 49 26.95 16.07 -20.63 26.18 16.71 -20.67 28.81 15.86 -22.27 29.37 16.78 -21.68 27.43 15.37 -21.82 26.37 15.70 -22.88 3 0 12.04-999 ?-999 ?-999 ? CH1 MLY B 25 mGLU.B 50 29.33 15.24 -23.33 28.72 14.58 -23.76 30.73 16.86 -24.57 30.62 16.90 -25.80 30.67 15.52 -23.82 31.15 14.37 -24.70 2 0 15.49-999 ?-999 ?-999 ? CH1 MLY B 25 mVAL.B 51 30.88 17.94 -23.82 30.91 17.75 -22.84 32.28 19.98 -23.90 32.43 20.25 -22.70 31.00 19.29 -24.39 29.73 20.17 -24.12 3 0 16.13-999 ?-999 ?-999 ? N MLY B 43 mTYR.B 52 33.18 20.23 -24.84 32.87 20.02 -25.77 34.81 21.92 -25.46 34.38 21.95 -26.60 34.49 20.75 -24.54 35.55 19.66 -24.68 3 0 15.27-999 ?-999 ?-999 ? C MLY B 43 mCYH.B 54 33.95 25.22 -25.64 34.06 24.97 -24.68 31.79 26.72 -25.28 30.62 26.55 -25.47 32.77 25.91 -26.15 31.96 24.89 -26.91 2 0 17.09-999 ?-999 ?-999 ? CH1 MLY B 71 mGLY.B 55 32.17 27.61 -24.37 31.40 28.12 -24.00 33.60 27.89 -22.43 34.26 27.05 -21.81 33.55 27.84 -23.95-999.99-999.99-999.99 1 0 15.39-999 ?-999 ?-999 ? CH1 MLY B 71 mLYS~B 57 32.01 27.24 -18.91 32.99 27.24 -18.72 30.00 25.74 -19.25 28.80 25.91 -19.02 31.06 26.31 -18.28 30.38 26.96 -17.06 2 0 9.24-999 ?-999 ?-999 ? CH1 MLY B 71 mPRO~B 58 30.44 25.06 -20.33 31.43 24.90 -20.35 28.43 23.61 -20.88 27.35 23.57 -21.45 29.51 24.59 -21.36 30.44 23.90 -22.38 3 0 11.75-999 ?-999 ?-999 ? CH1 MLY B 71 mTRP~B 59 28.74 22.84 -19.83 29.67 22.97 -19.49 26.60 22.64 -18.60 25.48 22.14 -18.59 27.78 21.90 -19.24 28.47 21.01 -18.20 3 0 10.45-999 ?-999 ?-999 ? CH1 MLY B 71 mGLU~B 60 26.87 23.83 -18.07 27.84 24.10 -18.13 25.00 25.36 -18.53 23.77 25.38 -18.44 25.84 24.67 -17.46 26.47 25.69 -16.50 2 0 7.59-999 ?-999 ?-999 ? CH1 MLY B 71 mVAL~B 61 25.67 25.91 -19.54 26.66 25.85 -19.45 24.05 25.52 -21.35 22.89 25.84 -21.63 25.00 26.53 -20.68 26.01 27.11 -21.71 3 0 10.48-999 ?-999 ?-999 ? CH1 MLY B 71 mTYR~B 62 24.55 24.31 -21.59 25.52 24.22 -21.34 22.48 22.97 -21.36 21.39 22.92 -21.93 23.76 23.22 -22.16 24.60 21.93 -22.23 3 0 12.73-999 ?-999 ?-999 ? CB MLY B 71 mALA~B 63 22.63 22.83 -20.04 23.58 22.92 -19.73 20.50 23.70 -19.14 19.29 23.47 -19.24 21.51 22.56 -19.15 22.02 22.28 -17.75 2 0 9.73-999 ?-999 ?-999 ? CB MLY B 71 mCYH~B 64 20.99 24.93 -19.04 21.99 24.97 -19.01 19.32 26.30 -20.27 18.16 26.74 -20.22 20.13 26.12 -18.99 20.95 27.36 -18.67 2 0 9.83-999 ?-999 ?-999 ? CB MLY B 71 mASN~B 65 19.92 25.95 -21.40 20.88 25.70 -21.29 18.16 24.86 -22.80 17.30 24.93 -23.68 19.23 25.95 -22.69 20.24 25.84 -23.84 2 0 13.51-999 ?-999 ?-999 ? N MLY B 71 mARG~B 66 18.22 23.87 -21.91 19.01 23.93 -21.30 16.27 22.97 -20.63 15.65 22.01 -20.18 17.20 22.81 -21.84 17.84 21.41 -21.81 2 0 12.68-999 ?-999 ?-999 ? N MLY B 71 mGLY.B 67 16.19 24.19 -20.10 16.82 24.85 -20.49 15.58 24.00 -17.65 14.69 23.72 -16.84 15.23 24.51 -19.03-999.99-999.99-999.99 1 0 10.72-999 ?-999 ?-999 ? N MLY B 71 mALA.B 68 16.88 23.88 -17.36 17.50 24.06 -18.13 16.76 24.53 -15.01 16.78 25.74 -15.25 17.32 23.53 -16.01 18.84 23.50 -15.93 2 0 7.05-999 ?-999 ?-999 ? N MLY B 71 mASN.B 69 16.24 24.01 -13.89 16.28 23.02 -13.84 16.09 24.51 -11.43 15.62 25.12 -10.46 15.65 24.86 -12.85 14.12 24.90 -12.96 2 0 5.65-999 ?-999 ?-999 ? N MLY B 71 mLEU^B 70 16.99 23.53 -11.31 17.23 23.09 -12.17 18.98 22.65 -10.14 19.24 21.68 -10.86 17.55 23.16 -10.01 16.68 22.13 -9.30 3 0 2.41-999 ?-999 ?-999 ? N MLY B 71 mILE^B 72 22.04 21.04 -8.07 21.79 21.51 -8.92 23.89 19.99 -6.86 24.35 19.26 -7.74 22.41 20.35 -6.84 21.56 19.07 -6.58 3 0 2.44-999 ?-999 ?-999 ? C MLY B 71 mSER^B 73 24.63 20.53 -5.89 24.15 21.18 -5.31 26.22 19.19 -4.60 25.59 19.33 -3.54 26.04 20.18 -5.74 26.90 21.43 -5.50 2 0 4.65-999 ?-999 ?-999 ? CA MLY B 8 mVAL^B 74 27.04 18.17 -4.83 27.39 18.15 -5.77 28.92 17.11 -3.79 29.53 16.60 -4.74 27.40 17.20 -3.80 26.75 15.80 -4.07 3 0 5.94-999 ?-999 ?-999 ? C MLY B 8 mASN.B 75 29.52 17.63 -2.72 28.89 17.84 -1.97 31.50 18.50 -3.95 31.05 19.59 -4.32 30.97 17.86 -2.66 31.72 16.58 -2.25 2 0 5.21-999 ?-999 ?-999 ? CH1 MLY B 8 mGLN.B 76 32.40 17.81 -4.66 32.61 16.91 -4.29 32.14 18.32 -7.14 32.52 18.87 -8.18 33.02 18.37 -5.88 34.37 17.69 -6.16 2 0 5.17-999 ?-999 ?-999 ? N MLY B 43 mPHE^B 77 30.99 17.66 -7.05 30.78 17.33 -6.13 28.98 18.47 -8.27 28.42 18.83 -7.23 30.12 17.45 -8.21 29.55 16.03 -8.21 3 0 6.92-999 ?-999 ?-999 ? N MLY B 43 mGLU^B 78 28.65 18.93 -9.47 29.28 18.66 -10.20 26.55 19.07 -10.67 26.99 18.61 -11.73 27.47 19.76 -9.68 27.82 21.17 -10.18 2 0 5.50-999 ?-999 ?-999 ? CE MLY B 71 mPHE^B 79 25.27 18.99 -10.31 25.08 19.41 -9.42 23.19 19.35 -11.50 22.66 20.05 -10.64 24.26 18.34 -11.13 23.61 17.18 -10.36 3 0 5.68-999 ?-999 ?-999 ? C MLY B 71 mTYR^B 80 22.86 19.42 -12.79 23.37 18.79 -13.38 20.61 19.58 -13.73 20.68 18.77 -14.66 21.85 20.34 -13.27 22.42 21.22 -14.39 3 0 4.69-999 ?-999 ?-999 ? CA MLY B 71 mPHE^B 81 19.49 19.84 -13.05 19.61 20.55 -12.36 17.17 20.09 -13.91 17.12 21.28 -13.58 18.22 19.16 -13.31 17.62 18.66 -11.99 3 0 5.91-999 ?-999 ?-999 ? N MLY B 71 mARG^B 82 16.34 19.54 -14.79 16.70 18.71 -15.24 14.00 19.30 -14.25 13.74 18.13 -14.54 15.02 20.10 -15.07 14.68 20.06 -16.57 2 0 8.11-999 ?-999 ?-999 ? N MLY B 71 mILE^B 83 13.46 19.93 -13.21 13.82 20.85 -13.05 11.09 19.50 -13.09 10.63 20.63 -13.28 12.43 19.31 -12.39 12.37 19.89 -10.95 3 0 8.98-999 ?-999 ?-999 ? N MLY B 71 mGLU^B 84 10.48 18.39 -13.49 11.00 17.55 -13.31 8.09 18.05 -13.15 8.25 17.13 -12.33 9.18 18.41 -14.15 9.15 17.46 -15.35 2 0 10.31-999 ?-999 ?-999 ? C MLY B 30 mVAL.B 85 6.99 18.79 -13.21 7.02 19.51 -13.90 4.58 18.27 -13.16 4.53 18.58 -14.36 5.83 18.56 -12.34 5.59 19.75 -11.37 3 0 9.53-999 ?-999 ?-999 ? O MLY B 30 mGLU.B 86 3.57 17.68 -12.52 3.74 17.54 -11.54 1.54 18.48 -13.68 0.89 18.42 -14.73 2.34 17.27 -13.20 1.48 16.38 -12.29 2 0 11.86-999 ?-999 ?-999 ? O MLY B 30 mGLY.B 87 1.60 19.56 -12.90 2.13 19.42 -12.07 -0.46 20.69 -13.77 -1.26 21.62 -13.72 0.96 20.84 -13.23-999.99-999.99-999.99 1 0 13.30-999 ?-999 ?-999 ? O MLY B 30