HEADER STRUCTURAL GENOMICS, UNKNOWN FUNCTION 02-AUG-07 2QTI COMPND UPF0352 PROTEIN SO_2176; COMPND 3 CHAIN: A; COMPND 4 ENGINEERED: YES SOURCE MOL_ID: 1; SOURCE 2 ORGANISM_SCIENTIFIC: SHEWANELLA ONEIDENSIS; SOURCE 3 ORGANISM_TAXID: 211586; SOURCE 4 STRAIN: MR-1; SOURCE 5 GENE: SO_2176; SOURCE 6 EXPRESSION_SYSTEM: ESCHERICHIA COLI; SOURCE 7 EXPRESSION_SYSTEM_TAXID: 562; SOURCE 8 EXPRESSION_SYSTEM_STRAIN: BL21(DE3)+MAGIC; SOURCE 9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID; SOURCE 10 EXPRESSION_SYSTEM_PLASMID: PET21 AUTHOR S.M.VOROBIEV,M.SU,J.SEETHARAMAN,A.P.KUZIN,D.WANG,K.CUNNINGHAM, AUTHOR 2 L.OWENS,M.MAGLAQUI,Y.FANG,R.XIAO,T.B.ACTON,G.T.MONTELIONE,L.TONG, AUTHOR 3 J.F.HUNT,NORTHEAST STRUCTURAL GENOMICS CONSORTIUM (NESG) HET MSE A 35 8 mSER~A 8 45.55 19.08 34.90 45.86 18.16 34.67 44.73 21.19 33.94 43.81 22.00 33.91 45.08 20.45 35.23 46.17 21.21 35.99 2 0 1000.00-999 ?-999 ?-999 ? mASN~A 9 45.47 20.88 32.88 46.18 20.20 33.05 43.83 21.17 31.09 43.15 22.01 30.50 45.23 21.51 31.58 46.27 21.04 30.58 2 0 1000.00-999 ?-999 ?-999 ? mTHR~A 10 43.42 19.93 31.33 44.08 19.36 31.81 41.04 20.16 31.75 39.91 20.38 31.31 42.10 19.48 30.90 41.97 17.95 31.06 2 0 1000.00-999 ?-999 ?-999 ? mGLN~A 11 41.42 20.51 32.98 42.37 20.27 33.20 40.30 22.61 33.47 39.17 23.10 33.47 40.53 21.18 33.92 41.14 21.16 35.31 2 0 1000.00-999 ?-999 ?-999 ? mVAL~A 12 41.38 23.28 33.08 42.23 22.76 33.14 40.52 24.68 31.31 39.56 25.45 31.15 41.30 24.66 32.61 42.71 25.24 32.35 3 0 1000.00-999 ?-999 ?-999 ? mGLU~A 13 40.94 23.82 30.39 41.72 23.26 30.67 38.81 23.42 29.16 38.03 23.98 28.39 40.31 23.72 29.08 41.04 22.66 28.25 2 0 1000.00-999 ?-999 ?-999 ? mSER~A 14 38.41 22.56 30.09 39.14 22.17 30.65 36.22 23.46 30.73 35.12 23.71 30.25 37.00 22.24 30.27 36.82 21.12 31.30 2 0 1000.00-999 ?-999 ?-999 ? mLEU~A 15 36.79 24.21 31.68 37.67 23.86 32.00 35.98 26.40 31.05 34.90 26.95 30.84 36.16 25.42 32.19 37.03 26.10 33.26 3 0 1000.00-999 ?-999 ?-999 ? mILE~A 16 37.06 26.58 30.30 37.86 26.05 30.60 36.02 27.10 28.14 35.23 27.95 27.70 37.09 27.48 29.16 38.48 27.44 28.49 3 0 1000.00-999 ?-999 ?-999 ? mALA~A 17 35.98 25.83 27.77 36.66 25.24 28.22 33.60 25.67 27.26 32.76 26.18 26.51 35.01 25.37 26.79 35.18 23.87 26.56 2 0 1000.00-999 ?-999 ?-999 ? mGLU~A 18 33.33 25.35 28.53 34.11 24.97 29.03 31.63 27.03 29.04 30.49 27.35 28.72 32.03 25.56 29.11 32.03 25.08 30.56 2 0 1000.00-999 ?-999 ?-999 ? mILE~A 19 32.57 27.91 29.34 33.45 27.52 29.60 32.08 29.77 27.85 31.19 30.55 27.55 32.28 29.34 29.29 33.43 30.15 29.90 3 0 1000.00-999 ?-999 ?-999 ? mLEU~A 20 32.90 29.25 26.94 33.58 28.60 27.29 31.45 29.22 24.94 30.88 29.94 24.12 32.78 29.64 25.54 33.94 29.07 24.72 3 0 1000.00-999 ?-999 ?-999 ? mVAL~A 21 30.97 28.04 25.33 31.55 27.55 25.99 28.58 28.48 25.29 27.63 28.73 24.55 29.70 27.54 24.83 29.44 26.11 25.34 3 0 1000.00-999 ?-999 ?-999 ? mVAL~A 22 28.69 28.99 26.51 29.50 28.68 27.02 27.60 31.11 26.10 26.50 31.53 25.74 27.70 29.92 27.04 28.10 30.42 28.45 3 0 1000.00-999 ?-999 ?-999 ? mLEU~A 23 28.75 31.64 25.70 29.57 31.20 26.07 28.25 32.40 23.41 27.48 33.15 22.81 28.79 32.78 24.79 30.23 33.31 24.64 3 0 1000.00-999 ?-999 ?-999 ? mGLU~A 24 28.64 31.22 22.93 29.26 30.72 23.53 26.68 30.48 21.61 26.01 30.78 20.63 28.19 30.73 21.63 28.97 29.46 21.27 2 0 1000.00-999 ?-999 ?-999 ? mLYS~A 25 26.14 29.94 22.70 26.78 29.73 23.43 23.88 30.97 22.62 22.78 30.95 22.05 24.70 29.69 22.77 24.32 29.01 24.09 2 0 1000.00-999 ?-999 ?-999 ? mHIS~A 26 24.40 32.07 23.15 25.27 31.94 23.61 24.06 34.06 21.73 23.47 35.09 21.41 23.72 33.37 23.05 24.16 34.29 24.20 3 0 1000.00-999 ?-999 ?-999 ? mLYS~A 27 25.02 33.51 21.00 25.41 32.68 21.38 25.84 35.53 20.07 25.69 36.44 19.26 25.46 34.10 19.74 24.32 34.08 18.70 2 0 1000.00-999 ?-999 ?-999 ? mALA.A 28 26.30 35.72 21.30 26.36 34.89 21.86 27.83 37.62 20.95 28.82 36.95 20.66 26.69 37.03 21.77 27.09 36.95 23.25 2 0 1000.00-999 ?-999 ?-999 ? mPRO~A 29 27.70 38.90 20.56 26.84 39.33 20.85 30.03 39.65 20.55 30.04 39.39 21.75 28.72 39.60 19.77 28.12 40.98 19.58 3 0 1000.00-999 ?-999 ?-999 ? mTHR~A 30 31.12 39.98 19.87 30.97 40.15 18.90 32.39 41.08 21.67 32.77 40.76 22.80 32.42 40.06 20.52 33.51 40.48 19.51 2 0 1000.00-999 ?-999 ?-999 ? mASP~A 31 31.94 42.30 21.39 31.65 42.42 20.44 31.08 42.92 23.63 31.46 43.20 24.77 31.89 43.35 22.41 31.30 44.63 21.80 2 0 1000.00-999 ?-999 ?-999 ? mLEU~A 32 29.96 42.24 23.39 29.76 42.09 22.42 29.83 40.69 25.28 29.75 40.65 26.51 29.11 41.77 24.48 27.80 41.20 23.93 3 0 1000.00-999 ?-999 ?-999 ? mSER~A 33 30.54 39.81 24.58 30.51 39.96 23.59 32.34 39.30 26.14 32.48 38.87 27.28 31.27 38.73 25.22 31.93 37.83 24.17 2 0 1000.00-999 ?-999 ?-999 ? mLEU~A 34 33.10 40.25 25.61 32.88 40.49 24.67 33.62 41.58 27.62 34.18 41.47 28.71 34.17 40.89 26.38 34.89 41.92 25.51 3 0 1000.00-999 ?-999 ?-999 ? mMETA 35 32.52 42.29 27.45 32.17 42.29 26.51 31.46 42.04 29.64 31.64 42.30 30.83 31.89 43.01 28.54 30.68 43.76 27.99 2 0 1000.00-999 ?-999 ?-999 ? mALA~A 36 30.88 40.91 29.24 30.78 40.81 28.25 31.67 39.30 30.90 31.70 39.17 32.13 30.45 39.91 30.21 29.65 38.82 29.50 2 0 1000.00-999 ?-999 ?-999 ? mLEU~A 37 32.67 38.93 30.10 32.51 39.10 29.13 34.59 39.29 31.58 35.08 38.89 32.63 33.88 38.33 30.64 34.81 37.91 29.50 3 0 1000.00-999 ?-999 ?-999 ? mGLY~A 38 34.63 40.57 31.22 34.20 40.77 30.34 34.53 41.67 33.38 35.15 41.74 34.45 35.27 41.55 32.06-999.99-999.99-999.99 1 0 1000.00-999 ?-999 ?-999 ? mASN~A 39 33.21 41.70 33.31 32.83 41.64 32.39 32.61 40.58 35.40 32.56 40.69 36.62 32.40 41.80 34.52 30.92 41.96 34.19 2 0 1000.00-999 ?-999 ?-999 ? mCYH~A 40 32.83 39.42 34.80 32.82 39.45 33.80 34.36 38.38 36.39 34.42 38.03 37.56 33.06 38.23 35.60 33.09 36.98 34.72 2 0 1000.00-999 ?-999 ?-999 ? mVAL~A 41 35.40 38.91 35.75 35.23 39.14 34.79 36.47 40.13 37.56 36.89 39.92 38.69 36.68 39.12 36.43 37.78 39.67 35.46 3 0 1000.00-999 ?-999 ?-999 ? mTHR~A 42 35.80 41.24 37.23 35.52 41.29 36.28 34.79 41.67 39.42 35.16 41.91 40.57 35.53 42.27 38.22 34.70 43.42 37.62 2 0 1000.00-999 ?-999 ?-999 ? mHIS~A 43 33.76 40.87 39.13 33.60 40.76 38.15 33.86 39.40 41.08 33.85 39.58 42.30 32.95 40.22 40.17 31.92 39.30 39.51 3 0 1000.00-999 ?-999 ?-999 ? mLEU~A 44 34.64 38.50 40.49 34.54 38.46 39.50 36.52 38.46 42.12 36.67 38.16 43.30 35.57 37.66 41.24 36.41 36.81 40.30 3 0 1000.00-999 ?-999 ?-999 ? mLEU~A 45 37.18 39.46 41.54 36.97 39.59 40.57 37.47 40.92 43.50 38.03 40.95 44.58 38.12 40.27 42.30 38.75 41.35 41.40 3 0 1000.00-999 ?-999 ?-999 ? mGLU~A 46 36.27 41.46 43.29 35.93 41.36 42.36 35.11 41.13 45.43 35.15 41.43 46.62 35.54 42.12 44.36 34.30 42.82 43.79 2 0 1000.00-999 ?-999 ?-999 ? mARG~A 47 34.73 39.93 45.00 34.80 39.79 44.01 35.31 37.98 46.54 35.06 37.36 47.57 34.26 38.92 45.94 33.14 38.09 45.29 2 0 1000.00-999 ?-999 ?-999 ? mLYS~A 48 36.49 37.87 45.93 36.61 38.43 45.11 38.89 37.50 46.65 39.78 36.78 47.09 37.48 36.96 46.48 37.51 35.67 45.65 2 0 1000.00-999 ?-999 ?-999 ? mVAL~A 49 39.10 38.76 46.31 38.29 39.24 45.95 40.33 40.59 47.37 39.53 41.49 47.15 40.41 39.37 46.46 41.00 39.80 45.09 3 0 1000.00-999 ?-999 ?-999 ? mPRO~A 50 41.17 40.62 48.42 41.76 39.82 48.49 41.29 43.07 48.63 42.10 43.24 47.72 41.19 41.74 49.36 42.43 41.44 50.20 3 0 1000.00-999 ?-999 ?-999 ? mSER~A 51 40.45 44.01 49.05 39.86 43.71 49.80 41.78 45.93 48.14 41.95 46.59 47.12 40.41 45.34 48.47 39.67 46.27 49.43 2 0 1000.00-999 ?-999 ?-999 ? mGLU~A 52 42.75 45.69 49.02 42.46 45.12 49.79 44.93 45.60 47.72 45.91 46.19 47.27 44.11 46.21 48.86 44.87 46.03 50.18 2 0 1000.00-999 ?-999 ?-999 ? mSER~A 53 44.54 44.41 47.26 43.72 44.04 47.69 44.52 43.71 44.87 45.08 43.44 43.80 45.29 43.74 46.19 45.61 42.31 46.62 2 0 1000.00-999 ?-999 ?-999 ? mARG~A 54 43.22 44.00 44.96 42.93 44.23 45.89 42.89 44.63 42.55 42.94 44.01 41.49 42.33 43.97 43.81 41.01 44.61 44.20 2 0 1000.00-999 ?-999 ?-999 ? mGLN~A 55 43.31 45.88 42.67 43.21 46.28 43.58 45.08 45.85 40.99 45.25 45.67 39.78 43.85 46.58 41.52 44.25 48.01 41.91 2 0 1000.00-999 ?-999 ?-999 ? mALA~A 56 45.95 45.42 41.91 45.69 45.63 42.86 46.84 43.40 40.81 47.41 43.11 39.76 47.17 44.71 41.54 48.02 44.43 42.79 2 0 1000.00-999 ?-999 ?-999 ? mVAL~A 57 45.92 42.61 41.35 45.52 42.93 42.22 44.94 41.70 39.31 45.20 41.03 38.32 45.55 41.36 40.68 44.53 40.54 41.49 3 0 1000.00-999 ?-999 ?-999 ? mALA~A 58 44.13 42.76 39.26 43.99 43.22 40.13 44.61 43.50 36.98 44.52 43.10 35.81 43.52 43.18 38.00 42.63 44.40 38.23 2 0 1000.00-999 ?-999 ?-999 ? mGLU~A 59 45.64 44.22 37.42 45.60 44.48 38.38 47.53 43.38 36.03 47.94 43.32 34.87 46.73 44.58 36.51 47.69 45.59 37.17 2 0 1000.00-999 ?-999 ?-999 ? mGLN~A 60 47.76 42.41 36.91 47.39 42.57 37.82 47.66 40.41 35.54 48.18 39.78 34.61 48.50 41.22 36.51 48.85 40.37 37.74 2 0 1000.00-999 ?-999 ?-999 ? mPHE~A 61 46.34 40.43 35.74 46.04 40.96 36.53 45.61 40.37 33.46 45.74 39.70 32.44 45.45 39.71 34.83 43.99 39.85 35.29 3 0 1000.00-999 ?-999 ?-999 ? mALA~A 62 45.63 41.70 33.47 45.55 42.11 34.37 47.08 42.22 31.52 47.11 42.06 30.29 45.76 42.47 32.23 45.60 43.95 32.53 2 0 1000.00-999 ?-999 ?-999 ? mLYS~A 63 48.17 42.20 32.28 48.01 42.35 33.25 49.51 40.59 31.04 49.97 40.44 29.91 49.49 41.97 31.69 50.56 42.03 32.78 2 0 1000.00-999 ?-999 ?-999 ? mALA~A 64 49.00 39.60 31.77 48.67 39.89 32.67 48.13 38.21 29.99 48.52 37.59 29.01 48.94 38.23 31.27 48.30 37.32 32.32 2 0 1000.00-999 ?-999 ?-999 ? mLEU~A 65 47.00 38.89 30.00 46.78 39.36 30.86 46.96 39.53 27.65 47.01 38.95 26.56 46.17 38.93 28.81 44.91 39.75 29.05 3 0 1000.00-999 ?-999 ?-999 ? mALA~A 66 47.56 40.69 27.89 47.43 41.04 28.82 49.46 40.49 26.33 49.64 40.36 25.11 48.34 41.39 26.86 48.93 42.67 27.42 2 0 1000.00-999 ?-999 ?-999 ? mGLN~A 67 50.21 39.88 27.24 49.97 40.07 28.19 50.75 37.84 26.03 51.29 37.46 25.00 51.31 39.01 26.83 52.07 38.47 28.04 2 0 1000.00-999 ?-999 ?-999 ? mSER~A 68 49.65 37.28 26.52 49.30 37.68 27.37 48.76 36.54 24.39 49.25 35.90 23.46 49.04 36.16 25.84 47.74 35.76 26.53 2 0 1000.00-999 ?-999 ?-999 ? mVAL~A 69 47.99 37.61 24.21 47.68 38.05 25.05 48.88 38.32 22.01 48.94 37.87 20.86 47.65 38.09 22.87 46.85 39.42 22.93 3 0 1000.00-999 ?-999 ?-999 ? mLYS~A 70 49.87 39.01 22.56 49.69 39.31 23.50 51.73 38.03 21.32 51.90 37.85 20.11 51.09 39.31 21.84 52.06 40.06 22.75 2 0 1000.00-999 ?-999 ?-999 ? mSER~A 71 52.07 37.13 22.24 51.86 37.40 23.18 51.85 35.14 20.83 52.39 34.56 19.89 52.69 35.88 21.86 52.89 34.99 23.09 2 0 1000.00-999 ?-999 ?-999 ? mASN~A 72 50.53 35.17 20.99 50.21 35.68 21.79 49.86 35.07 18.65 49.49 34.45 17.65 49.65 34.49 20.04 48.18 34.65 20.45 2 0 1000.00-999 ?-999 ?-999 ? mLEU~A 73 50.45 36.26 18.60 50.66 36.65 19.49 52.12 36.42 16.88 52.99 37.22 16.54 50.75 36.91 17.33 50.77 38.44 17.51 3 0 1000.00-999 ?-999 ?-999 ? mGLU.A 74 52.29 35.10 16.88 51.49 34.59 17.19 53.32 33.50 15.32 52.15 33.21 15.00 53.54 34.49 16.47 54.19 33.78 17.66 2 0 1000.00-999 ?-999 ?-999 ?