HEADER UNKNOWN FUNCTION 07-MAY-07 2PST COMPND HYPOTHETICAL PROTEIN PA2412; COMPND 3 CHAIN: X; COMPND 4 ENGINEERED: YES SOURCE MOL_ID: 1; SOURCE 2 ORGANISM_SCIENTIFIC: PSEUDOMONAS AERUGINOSA PAO1; SOURCE 3 ORGANISM_TAXID: 208964; SOURCE 4 STRAIN: PA01; SOURCE 5 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3); SOURCE 6 EXPRESSION_SYSTEM_TAXID: 469008; SOURCE 7 EXPRESSION_SYSTEM_STRAIN: BL21(DE3); SOURCE 8 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID; SOURCE 9 EXPRESSION_SYSTEM_PLASMID: PET15 MODIFIED AUTHOR A.M.GULICK,E.J.DRAKE,M.B.SHAH mASP.X 8 9.82 36.82 37.78 10.27 35.99 38.09 9.66 39.33 38.03 9.79 39.38 39.26 9.14 38.07 37.33 7.65 37.96 37.49 2 0 1000.00-999 ?-999 ?-999 ? mASP.X 9 9.95 40.32 37.20 9.82 40.06 36.24 10.39 42.44 36.28 10.30 43.68 36.24 10.39 41.65 37.58 11.80 41.64 38.22 2 0 1000.00-999 ?-999 ?-999 ? mILE.X 10 10.34 41.68 35.19 10.27 40.70 35.38 8.97 42.22 33.23 8.29 41.19 33.22 10.36 42.24 33.85 11.39 41.49 32.94 3 0 1000.00-999 ?-999 ?-999 ? mGLN.X 11 8.55 43.38 32.73 9.14 44.15 32.95 7.61 42.95 30.54 8.55 43.38 29.87 7.33 43.48 31.94 6.86 44.93 31.88 2 0 1000.00-999 ?-999 ?-999 ? mPHE^X 12 6.78 42.00 30.11 6.14 41.67 30.81 5.60 41.97 27.97 4.61 42.44 28.55 6.79 41.47 28.76 6.75 39.94 28.77 3 0 1000.00-999 ?-999 ?-999 ? mGLN^X 13 5.71 41.88 26.65 6.63 41.66 26.34 4.37 40.79 25.02 5.35 40.17 24.61 4.58 42.10 25.76 4.93 43.13 24.70 2 0 1000.00-999 ?-999 ?-999 ? mVAL^X 14 3.11 40.40 24.84 2.44 40.90 25.38 2.89 39.85 22.51 2.34 40.92 22.23 2.80 39.30 23.92 1.40 38.69 24.16 3 0 1000.00-999 ?-999 ?-999 ? mVAL^X 15 3.60 39.15 21.64 4.00 38.31 22.01 3.27 38.51 19.32 3.24 37.33 19.69 3.77 39.60 20.26 5.22 40.03 19.93 3 0 1000.00-999 ?-999 ?-999 ? mVAL^X 16 2.85 38.92 18.12 2.98 39.90 17.98 2.84 38.35 15.75 3.10 39.53 15.45 2.25 38.01 17.13 0.69 38.13 17.15 3 0 1000.00-999 ?-999 ?-999 ? mASN^X 17 3.09 37.30 14.96 2.90 36.41 15.37 2.50 37.35 12.55 1.32 37.26 12.89 3.61 37.48 13.60 4.80 36.54 13.34 2 0 1000.00-999 ?-999 ?-999 ? mHIS.X 18 2.86 37.35 11.27 3.85 37.37 11.12 1.21 35.92 10.04 0.18 35.78 9.38 1.86 37.31 10.21 2.47 37.85 8.90 3 0 1000.00-999 ?-999 ?-999 ? mGLU.X 19 1.80 34.90 10.66 2.67 35.14 11.08 0.32 33.33 11.94 -0.14 32.20 12.19 1.19 33.56 10.70 2.27 32.48 10.60 2 0 1000.00-999 ?-999 ?-999 ? mGLU.X 20 0.06 34.40 12.69 0.47 35.24 12.34 -0.15 33.29 14.88 -0.85 32.47 15.48 -0.73 34.33 13.90 -2.22 34.13 13.57 2 0 1000.00-999 ?-999 ?-999 ? mGLN.X 21 1.18 33.35 15.01 1.61 33.94 14.33 2.40 33.66 17.07 2.68 34.81 16.73 1.96 32.64 16.02 3.19 32.02 15.38 2 0 1000.00-999 ?-999 ?-999 ? mTYR^X 22 2.48 33.22 18.33 2.40 32.23 18.42 3.98 33.88 20.17 4.44 32.74 20.27 2.68 34.14 19.45 1.50 34.06 20.46 3 0 1000.00-999 ?-999 ?-999 ? mSER^X 23 4.55 34.95 20.70 4.14 35.82 20.44 5.62 35.90 22.66 4.68 36.72 22.68 5.70 34.82 21.58 7.01 34.91 20.77 2 0 1000.00-999 ?-999 ?-999 ? mILE^X 24 6.57 35.88 23.58 7.16 35.08 23.55 7.99 37.73 24.24 8.96 37.14 23.75 6.70 36.97 24.55 6.66 36.49 26.02 3 0 1000.00-999 ?-999 ?-999 ? mTRP^X 25 7.98 39.03 24.51 7.16 39.36 24.97 9.24 40.94 25.27 8.24 41.56 25.67 9.09 39.89 24.16 8.84 40.54 22.78 3 0 1000.00-999 ?-999 ?-999 ? mPRO^X 26 10.47 41.16 25.75 11.22 40.65 25.33 10.19 43.50 26.36 10.57 43.92 25.27 10.63 42.11 26.84 12.14 42.10 27.12 3 0 1000.00-999 ?-999 ?-999 ? mGLU.X 27 9.35 44.17 27.16 9.07 43.66 27.97 10.05 46.51 26.58 9.91 47.43 25.78 8.90 45.52 26.85 8.04 46.05 27.99 2 0 1000.00-999 ?-999 ?-999 ? mTYR.X 28 11.18 46.28 27.24 11.17 45.48 27.84 13.03 47.06 25.72 13.80 47.94 25.36 12.33 47.17 27.08 13.32 46.97 28.24 3 0 1000.00-999 ?-999 ?-999 ? mLYS.X 29 12.72 46.01 24.96 12.09 45.35 25.37 12.32 46.26 22.51 11.11 46.04 22.60 13.29 45.84 23.63 13.77 44.40 23.41 2 0 1000.00-999 ?-999 ?-999 ? mGLU.X 30 12.89 46.86 21.47 13.84 47.12 21.61 11.56 45.82 19.64 12.24 44.79 19.58 12.15 47.12 20.24 13.06 47.79 19.21 2 0 1000.00-999 ?-999 ?-999 ? mILE.X 31 10.30 45.88 19.24 9.86 46.76 19.40 10.30 44.45 17.24 10.43 45.37 16.42 9.64 44.73 18.60 8.10 44.90 18.49 3 0 1000.00-999 ?-999 ?-999 ? mPRO.X 32 10.74 43.19 17.00 10.54 42.51 17.71 10.54 43.01 14.53 9.33 42.82 14.66 11.46 42.89 15.75 11.92 41.43 15.93 3 0 1000.00-999 ?-999 ?-999 ? mGLN.X 33 11.11 43.35 13.38 12.08 43.60 13.45 9.62 42.04 11.92 10.17 40.97 12.15 10.36 43.37 12.12 11.29 43.66 10.94 2 0 1000.00-999 ?-999 ?-999 ? mGLY.X 34 8.36 42.11 11.48 8.03 43.04 11.35 6.64 40.59 12.38 5.82 39.66 12.28 7.57 40.92 11.22-999.99-999.99-999.99 1 0 1000.00-999 ?-999 ?-999 ? mTRP^X 35 6.80 41.34 13.48 7.49 42.05 13.38 5.34 42.42 15.14 5.81 43.53 14.81 6.01 41.13 14.70 6.90 40.60 15.85 3 0 1000.00-999 ?-999 ?-999 ? mARG^X 36 4.24 42.28 15.87 3.94 41.33 15.99 2.97 43.04 17.80 2.83 41.85 18.11 3.56 43.43 16.44 2.47 43.96 15.49 2 0 1000.00-999 ?-999 ?-999 ? mALA^X 37 2.62 44.06 18.59 2.86 44.96 18.23 0.61 43.18 19.67 -0.20 43.60 18.83 1.96 43.85 19.87 1.81 45.19 20.62 2 0 1000.00-999 ?-999 ?-999 ? mALA^X 38 0.37 42.12 20.43 1.11 41.89 21.07 -1.94 41.80 21.32 -3.01 41.19 21.36 -0.87 41.36 20.33 -0.60 39.85 20.47 2 0 1000.00-999 ?-999 ?-999 ? mGLY^X 39 -1.65 42.82 22.13 -0.71 43.16 22.04 -2.58 42.85 24.49 -3.61 42.77 25.17 -2.64 43.37 23.06-999.99-999.99-999.99 1 0 1000.00-999 ?-999 ?-999 ? mLYS^X 40 -1.38 42.53 24.95 -0.64 42.57 24.28 0.26 42.44 26.80 1.21 41.98 26.16 -1.18 42.14 26.35 -1.51 40.65 26.55 2 0 1000.00-999 ?-999 ?-999 ? mSER^X 41 0.39 43.22 27.88 -0.47 43.64 28.17 1.54 42.95 29.99 0.44 42.95 30.55 1.67 43.43 28.56 2.07 44.92 28.61 2 0 1000.00-999 ?-999 ?-999 ? mGLY^X 42 2.65 42.56 30.59 3.48 42.61 30.03 3.80 41.25 32.34 4.74 41.09 31.55 2.62 42.12 31.98-999.99-999.99-999.99 1 0 1000.00-999 ?-999 ?-999 ? mLEU~X 43 3.75 40.70 33.55 2.98 41.00 34.12 4.64 38.48 33.12 3.62 38.22 32.49 4.74 39.74 33.97 4.58 39.43 35.46 3 0 1000.00-999 ?-999 ?-999 ? mLYS~X 44 5.73 37.72 33.08 6.46 38.04 33.68 4.62 35.60 32.40 4.03 35.18 31.39 5.83 36.53 32.25 7.13 35.80 32.56 2 0 1000.00-999 ?-999 ?-999 ? mLYS~X 45 4.26 35.25 33.64 4.79 35.69 34.37 1.83 34.81 33.26 1.08 34.02 32.69 3.16 34.31 33.85 3.02 33.93 35.34 2 0 1000.00-999 ?-999 ?-999 ? mASP~X 46 1.56 36.11 33.37 2.27 36.64 33.83 0.33 36.69 31.31 -0.69 36.40 30.68 0.32 36.67 32.83 0.06 38.05 33.43 2 0 1000.00-999 ?-999 ?-999 ? mCYH~X 47 1.48 36.99 30.73 2.21 37.27 31.36 1.44 35.47 28.77 0.76 35.24 27.78 1.62 36.91 29.27 2.95 37.50 28.82 2 0 1000.00-999 ?-999 ?-999 ? mLEU~X 48 2.03 34.51 29.50 2.53 34.84 30.30 0.49 32.60 29.25 0.02 31.86 28.39 1.92 33.10 29.11 2.92 32.23 29.91 3 0 1000.00-999 ?-999 ?-999 ? mALA~X 49 -0.20 33.03 30.30 0.33 33.60 30.93 -2.46 33.22 29.33 -3.30 32.51 28.79 -1.62 32.71 30.51 -2.12 33.28 31.83 2 0 1000.00-999 ?-999 ?-999 ? mTYR~X 50 -2.20 34.47 28.93 -1.51 34.94 29.48 -2.69 34.29 26.49 -3.66 33.99 25.78 -2.87 35.08 27.79 -2.38 36.52 27.65 3 0 1000.00-999 ?-999 ?-999 ? mILE~X 51 -1.44 33.96 26.17 -0.75 34.30 26.81 -1.91 31.84 25.02 -2.53 31.44 24.05 -1.14 33.17 24.97 0.39 32.92 24.80 3 0 1000.00-999 ?-999 ?-999 ? mGLU~X 52 -1.87 31.17 26.17 -1.30 31.58 26.87 -4.14 30.12 26.08 -4.79 29.25 25.49 -2.63 29.93 26.33 -2.38 29.33 27.71 2 0 1000.00-999 ?-999 ?-999 ? mGLU~X 53 -4.68 31.25 26.53 -4.03 31.85 27.00 -6.46 31.81 24.86 -7.46 31.28 24.37 -6.11 31.59 26.35 -6.46 32.83 27.20 2 0 1000.00-999 ?-999 ?-999 ? mVAL~X 54 -5.59 32.53 24.14 -4.73 32.72 24.61 -5.40 32.08 21.63 -5.97 32.07 20.54 -5.91 32.99 22.77 -5.47 34.48 22.54 3 0 1000.00-999 ?-999 ?-999 ? mTRP~X 55 -4.33 31.34 21.88 -3.98 31.41 22.82 -4.21 29.04 20.99 -3.52 28.19 21.55 -3.74 30.49 20.84 -2.21 30.60 20.86 3 0 1000.00-999 ?-999 ?-999 ? mTHR.X 56 -5.41 28.77 20.50 -5.83 29.53 20.01 -5.90 26.60 19.41 -5.93 25.37 19.49 -6.02 27.45 20.67 -7.50 27.58 21.07 2 0 1000.00-999 ?-999 ?-999 ? mASP.X 57 -5.76 27.26 18.27 -5.83 28.25 18.36 -4.10 26.90 16.60 -3.80 28.00 16.14 -5.53 26.57 16.99 -6.51 27.08 15.92 2 0 1000.00-999 ?-999 ?-999 ? mMET.X 58 -3.20 25.96 16.83 -3.56 25.08 17.13 -1.23 26.05 15.25 -0.01 26.20 15.04 -1.80 26.29 16.65 -0.96 25.64 17.70 2 0 1000.00-999 ?-999 ?-999 ? mARG.X 59 -2.10 25.74 14.30 -3.02 25.53 14.62 -1.33 27.16 12.49 -2.03 28.10 12.88 -1.68 25.72 12.90 -2.82 25.21 12.01 2 0 1000.00-999 ?-999 ?-999 ? mPRO~X 60 -0.25 27.34 11.71 0.31 26.53 11.52 -1.21 29.14 10.39 -1.89 28.30 9.78 0.00 28.68 11.19 1.19 28.49 10.25 3 0 1000.00-999 ?-999 ?-999 ? mLEU~X 61 -1.45 30.44 10.37 -0.80 30.98 10.90 -2.66 30.49 8.15 -3.75 30.16 7.67 -2.58 31.00 9.60 -2.53 32.53 9.62 3 0 1000.00-999 ?-999 ?-999 ? mSER~X 62 -1.52 30.44 7.45 -0.72 30.76 7.96 -1.93 28.51 5.93 -2.56 28.13 4.94 -1.46 29.96 6.06 -0.04 30.05 5.50 2 0 1000.00-999 ?-999 ?-999 ? mLEU~X 63 -1.60 27.71 6.93 -1.03 28.14 7.63 -3.57 26.21 7.17 -4.24 25.46 6.46 -2.04 26.31 6.96 -1.26 25.51 8.01 3 0 1000.00-999 ?-999 ?-999 ? mARG~X 64 -4.10 26.95 8.14 -3.43 27.45 8.69 -6.28 27.38 7.05 -7.26 26.75 6.66 -5.57 27.02 8.34 -5.93 28.05 9.41 2 0 1000.00-999 ?-999 ?-999 ? mGLN~X 65 -5.77 28.41 6.38 -4.97 28.82 6.81 -6.32 27.79 4.04 -7.27 27.67 3.25 -6.34 28.87 5.11 -5.59 30.11 4.63 2 0 1000.00-999 ?-999 ?-999 ? mHIS~X 66 -5.26 27.00 4.01 -4.56 27.22 4.68 -6.05 24.68 3.39 -6.55 23.99 2.48 -5.17 25.90 3.06 -3.71 25.53 2.82 3 0 1000.00-999 ?-999 ?-999 ? mMET~X 67 -6.26 24.43 4.68 -5.91 25.13 5.30 -8.48 23.46 5.22 -9.27 22.54 4.97 -6.98 23.23 5.11 -6.44 22.76 6.46 2 0 1000.00-999 ?-999 ?-999 ? mASP~X 68 -8.84 24.69 5.57 -8.09 25.35 5.59 -10.89 25.74 4.73 -11.79 26.56 4.91 -10.22 25.04 5.91 -10.26 25.93 7.17 2 0 1000.00-999 ?-999 ?-999 ?