REMARK SOURCE hebe.pdb HEADER STRUCTURAL GENOMICS, UNKNOWN FUNCTION 17-APR-07 2PK7 COMPND UNCHARACTERIZED PROTEIN; COMPND 3 CHAIN: A, B; COMPND 4 ENGINEERED: YES SOURCE MOL_ID: 1; SOURCE 2 ORGANISM_SCIENTIFIC: PSEUDOMONAS FLUORESCENS; SOURCE 3 ORGANISM_TAXID: 220664; SOURCE 4 STRAIN: PF-5; SOURCE 5 ATCC: BAA-477; SOURCE 6 GENE: PFL_1779; SOURCE 7 EXPRESSION_SYSTEM: ESCHERICHIA COLI; SOURCE 8 EXPRESSION_SYSTEM_TAXID: 562; SOURCE 9 EXPRESSION_SYSTEM_STRAIN: BL21(DE3)+MAGIC; SOURCE 10 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID; SOURCE 11 EXPRESSION_SYSTEM_PLASMID: PET21 AUTHOR S.M.VOROBIEV,H.NEELY,S.JAYARAMAN,C.X.CHEN,H.JANJUA,R.XIAO,T.ACTON, AUTHOR 2 G.T.MONTELIONE,J.F.HUNT,L.TONG,NORTHEAST STRUCTURAL GENOMICS AUTHOR 3 CONSORTIUM (NESG) HET MSE A 45 8 HET MSE B 45 8 >LEU~A 5 4.63 -9.22 -0.92 3.34 -8.37 0.59 3.98 -8.80 -0.16 3 0 1000.00 -999 ? >LEU~A 6 5.92 -6.74 -5.50 6.84 -4.85 -5.11 6.38 -5.79 -5.31 3 0 1000.00 -999 ? >ASP~A 7 3.08 -11.01 -7.65 5.09 -11.33 -8.13 4.09 -11.17 -7.89 2 0 1000.00 -999 ? >ILE~A 8 -0.38 -10.33 -3.86 -0.57 -11.38 -3.16 -0.48 -10.85 -3.51 3 0 1000.00 -999 ? >LEU.A 9 0.66 -5.20 -3.16 0.80 -5.73 -1.06 0.73 -5.47 -2.11 3 0 1000.00 -999 ? >ALA.A 10 -2.03 -3.19 -6.29 -3.30 -3.68 -7.00 -2.67 -3.44 -6.64 2 0 1000.00 -999 ? >CYH.A 11 -2.22 0.59 -6.25 -2.46 3.20 -7.30 -2.34 1.89 -6.77 2 0 1000.00 -999 ? >PRO.A 12 -4.06 2.70 -2.88 -4.72 1.44 -4.59 -4.39 2.07 -3.73 3 0 1000.00 -999 ? >ILE.A 13 -5.24 5.59 -7.03 -6.00 6.72 -6.38 -5.62 6.15 -6.70 3 0 1000.00 -999 ? >CYH.A 14 -5.44 2.93 -10.40 -2.71 2.75 -10.83 -4.07 2.84 -10.61 2 0 1000.00 -999 ? >LYS.A 15 -4.98 -0.75 -9.49 -7.29 -2.10 -5.73 -6.14 -1.42 -7.61 2 0 1000.00 -999 ? >GLY.A 16 -1.73 -0.89 -11.42 -1.75 -0.96 -10.58 -1.74 -0.93 -11.00 1 0 1000.00 -999 ? >PRO.A 17 2.30 -2.00 -12.54 1.03 -2.20 -10.72 1.66 -2.10 -11.63 3 0 1000.00 -999 ? >LEU.A 18 3.12 -1.24 -6.07 1.42 -0.28 -5.17 2.27 -0.76 -5.62 3 0 1000.00 -999 ? >LYS^A 19 7.49 -0.99 -7.32 9.01 1.86 -10.29 8.25 0.43 -8.81 2 0 1000.00 -999 ? >LEU^A 20 10.41 -1.88 -3.58 10.76 -2.03 -1.45 10.58 -1.95 -2.52 3 0 1000.00 -999 ? >SER.A 21 11.53 2.60 -4.55 11.49 3.66 -2.38 11.51 3.13 -3.46 2 0 1000.00 -999 ? >ALA.A 22 15.32 2.77 -4.82 16.08 3.85 -5.59 15.70 3.31 -5.20 2 0 1000.00 -999 ? >ASP.A 23 15.85 5.18 -0.60 13.94 6.08 -0.65 14.89 5.63 -0.63 2 0 1000.00 -999 ? >LYS.A 24 13.16 1.16 -0.27 15.54 -2.15 -3.04 14.35 -0.49 -1.65 2 0 1000.00 -999 ? >THR.A 25 11.03 3.65 1.65 12.33 5.33 1.91 11.68 4.49 1.78 2 0 1000.00 -999 ? >GLU^A 26 8.59 6.44 -0.70 8.89 8.16 -1.91 8.74 7.30 -1.31 2 0 1000.00 -999 ? >LEU^A 27 4.69 1.05 -2.44 4.94 -0.81 -1.40 4.81 0.12 -1.92 3 0 1000.00 -999 ? >ILE^A 28 6.92 4.36 -6.97 7.92 4.35 -8.00 7.42 4.36 -7.48 3 0 1000.00 -999 ? >SER^A 29 2.58 3.70 -8.40 0.20 3.61 -8.65 1.39 3.66 -8.52 2 0 1000.00 -999 ? >GLY.A 31 0.01 4.24 -12.99 0.25 4.76 -12.38 0.13 4.49 -12.68 1 0 1000.00 -999 ? >ALA.A 32 -1.19 6.84 -10.49 -1.72 6.35 -9.15 -1.46 6.59 -9.82 2 0 1000.00 -999 ? >GLY.A 33 1.85 9.08 -10.39 1.50 8.69 -9.74 1.67 8.89 -10.07 1 0 1000.00 -999 ? >LEU^A 34 0.80 8.44 -5.92 -0.90 9.76 -5.89 -0.05 9.10 -5.90 3 0 1000.00 -999 ? >ALA^A 35 4.59 7.07 -4.13 6.03 7.63 -4.06 5.31 7.35 -4.10 2 0 1000.00 -999 ? >TYR^A 36 1.02 4.53 -1.99 -1.05 4.82 -3.87 -0.01 4.68 -2.93 3 0 1000.00 -999 ? >PRO^A 37 3.79 7.37 2.96 4.88 5.98 1.59 4.33 6.68 2.27 3 0 1000.00 -999 ? >ILE^A 38 7.39 1.89 3.66 7.68 0.59 3.14 7.53 1.24 3.40 3 0 1000.00 -999 ? >ARG^A 39 4.20 7.28 7.19 2.92 9.77 5.97 3.56 8.53 6.58 2 0 1000.00 -999 ? >ASP.A 40 7.20 2.56 11.85 7.99 1.67 13.58 7.59 2.12 12.72 2 0 1000.00 -999 ? >GLY.A 41 7.11 -1.14 8.58 6.64 -0.53 8.54 6.88 -0.84 8.56 1 0 1000.00 -999 ? >ILE^A 42 2.67 0.50 8.41 1.81 -0.65 8.74 2.24 -0.07 8.58 3 0 1000.00 -999 ? >PRO^A 43 2.15 -1.35 3.41 2.99 0.48 4.37 2.57 -0.43 3.89 3 0 1000.00 -999 ? >VAL.A 44 0.55 3.83 4.14 0.42 5.31 5.72 0.48 4.57 4.93 3 0 1000.00 -999 ? >MET?A 45 -1.31 3.65 0.88 0.05 0.00 -0.55 -0.63 1.82 0.16 2 0 1000.00 -999 ? >LEU~A 46 -4.11 7.11 2.21 -5.38 6.26 3.75 -4.74 6.68 2.98 3 0 1000.00 -999 ? >GLU~A 47 -4.06 10.77 -3.78 -5.55 11.12 -5.22 -4.81 10.94 -4.50 2 0 1000.00 -999 ? >SER~A 48 -3.65 11.91 1.07 -4.80 10.70 2.81 -4.22 11.30 1.94 2 0 1000.00 -999 ? >GLU~A 49 -1.42 9.43 4.57 -1.04 11.03 5.89 -1.23 10.23 5.23 2 0 1000.00 -999 ? >ALA~A 50 1.50 9.93 -0.78 0.84 9.20 -1.94 1.17 9.57 -1.36 2 0 1000.00 -999 ? >ARG^A 51 8.05 12.25 -2.81 8.77 10.36 -5.10 8.41 11.31 -3.95 2 0 1000.00 -999 ? >THR^A 52 3.37 12.80 -6.18 2.62 14.84 -6.39 3.00 13.82 -6.28 2 0 1000.00 -999 ? >LEU.A 53 5.86 10.36 -9.53 5.82 8.29 -8.89 5.84 9.32 -9.21 3 0 1000.00 -999 ? >THR~A 54 8.38 14.10 -10.11 10.35 13.95 -9.22 9.36 14.03 -9.66 2 0 1000.00 -999 ? >THR~A 55 8.56 14.87 -13.79 8.52 16.70 -14.91 8.54 15.78 -14.35 2 0 1000.00 -999 ? >GLU~A 57 10.65 10.13 -9.81 9.88 10.07 -7.84 10.26 10.10 -8.82 2 0 1000.00 -999 ? >ARG~A 58 4.93 12.51 -12.74 2.03 13.24 -12.18 3.48 12.87 -12.46 2 0 1000.00 -999 ? >LEU~A 59 10.26 10.67 -18.05 10.17 12.65 -18.85 10.21 11.66 -18.45 3 0 1000.00 -999 ? >ASP.A 60 9.55 6.02 -20.75 7.77 4.91 -20.85 8.66 5.47 -20.80 2 0 1000.00 -999 ? >GLU.A 63 18.77 0.16 -21.11 18.84 -1.59 -22.28 18.81 -0.72 -21.70 2 0 1000.00 -999 ? >HIS.A 64 18.81 1.88 -25.83 16.89 1.06 -26.04 17.85 1.47 -25.94 3 0 1000.00 -999 ? >HIS.A 65 23.98 6.20 -26.01 25.81 5.28 -25.58 24.90 5.74 -25.80 3 0 1000.00 -999 ? >LYS~B 4 12.05 -7.59 -0.80 14.06 -13.10 0.55 13.06 -10.35 -0.12 2 0 1000.00 -999 ? >LEU~B 5 7.60 -5.75 -0.15 6.29 -4.12 0.42 6.95 -4.94 0.14 3 0 1000.00 -999 ? >LEU~B 6 8.02 -10.14 2.82 6.32 -11.20 3.67 7.17 -10.67 3.24 3 0 1000.00 -999 ? >ASP~B 7 12.96 -7.99 4.07 13.31 -9.89 3.23 13.14 -8.94 3.65 2 0 1000.00 -999 ? >ILE~B 8 11.23 -3.13 4.76 11.66 -2.66 3.44 11.45 -2.90 4.10 3 0 1000.00 -999 ? >LEU~B 9 6.06 -5.11 5.88 5.19 -4.07 4.18 5.62 -4.59 5.03 3 0 1000.00 -999 ? >ALA.B 10 6.69 -5.30 10.34 7.68 -4.51 11.19 7.18 -4.90 10.76 2 0 1000.00 -999 ? >CYH.B 11 3.80 -6.06 12.65 2.23 -7.26 14.61 3.01 -6.66 13.63 2 0 1000.00 -999 ? >PRO.B 12 0.06 -4.05 12.52 2.06 -3.57 13.40 1.06 -3.81 12.96 3 0 1000.00 -999 ? >ILE.B 13 0.28 -5.59 17.43 -0.57 -4.91 18.23 -0.15 -5.25 17.83 3 0 1000.00 -999 ? >CYH.B 14 4.59 -6.17 18.00 4.75 -8.69 16.76 4.67 -7.43 17.38 2 0 1000.00 -999 ? >LYS.B 15 6.96 -4.87 15.30 8.32 -0.43 15.83 7.64 -2.65 15.57 2 0 1000.00 -999 ? >GLY.B 16 7.71 -8.51 14.52 7.32 -8.04 13.98 7.51 -8.27 14.25 1 0 1000.00 -999 ? >PRO.B 17 9.43 -11.80 12.36 8.35 -9.94 11.78 8.89 -10.87 12.07 3 0 1000.00 -999 ? >LEU.B 18 4.65 -9.82 8.15 3.43 -8.39 9.20 4.04 -9.11 8.68 3 0 1000.00 -999 ? >LYS^B 19 4.97 -13.99 6.53 6.02 -17.22 10.45 5.50 -15.61 8.49 2 0 1000.00 -999 ? >LEU^B 20 3.15 -14.27 2.05 1.89 -13.63 0.41 2.52 -13.95 1.23 3 0 1000.00 -999 ? >SER.B 21 0.00 -16.88 4.50 -2.17 -16.25 3.62 -1.09 -16.56 4.06 2 0 1000.00 -999 ? >ALA.B 22 -0.15 -20.11 2.51 -0.30 -21.51 3.13 -0.22 -20.81 2.82 2 0 1000.00 -999 ? >ASP.B 23 -4.82 -19.19 1.35 -5.25 -17.71 2.80 -5.03 -18.45 2.07 2 0 1000.00 -999 ? >LYS.B 24 -1.66 -15.73 0.26 -4.23 -15.25 -4.97 -2.94 -15.49 -2.35 2 0 1000.00 -999 ? >THR.B 25 -4.61 -13.63 1.48 -6.28 -15.01 1.62 -5.44 -14.32 1.55 2 0 1000.00 -999 ? >GLU^B 26 -5.10 -13.58 5.85 -5.70 -15.07 7.24 -5.40 -14.32 6.54 2 0 1000.00 -999 ? >LEU^B 27 0.66 -9.89 6.27 1.33 -8.90 4.47 0.99 -9.39 5.37 3 0 1000.00 -999 ? >ILE^B 28 1.18 -15.07 9.28 -0.11 -15.66 9.59 0.54 -15.36 9.43 3 0 1000.00 -999 ? >SER^B 29 2.34 -12.11 12.56 2.74 -10.34 14.17 2.54 -11.23 13.37 2 0 1000.00 -999 ? >LYS.B 30 3.18 -15.01 14.86 3.40 -20.92 13.44 3.29 -17.96 14.15 2 0 1000.00 -999 ? >GLY.B 31 4.81 -12.43 17.10 4.02 -12.58 16.92 4.41 -12.51 17.01 1 0 1000.00 -999 ? >ALA.B 32 1.28 -11.20 17.81 0.90 -9.87 17.15 1.09 -10.54 17.48 2 0 1000.00 -999 ? >GLY.B 33 -0.91 -14.23 17.09 -0.88 -13.46 16.69 -0.90 -13.85 16.89 1 0 1000.00 -999 ? >LEU^B 34 -2.53 -10.69 14.64 -3.81 -9.75 16.10 -3.17 -10.22 15.37 3 0 1000.00 -999 ? >ALA^B 35 -3.16 -12.29 10.54 -3.81 -13.57 10.05 -3.48 -12.93 10.29 2 0 1000.00 -999 ? >TYR^B 36 -2.04 -7.61 9.98 -1.00 -7.10 12.53 -1.52 -7.35 11.26 3 0 1000.00 -999 ? >PRO^B 37 -7.45 -7.96 6.94 -5.66 -9.17 6.34 -6.56 -8.57 6.64 3 0 1000.00 -999 ? >ILE^B 38 -3.89 -9.10 1.33 -2.57 -9.23 0.74 -3.23 -9.16 1.04 3 0 1000.00 -999 ? >ARG^B 39 -11.28 -6.83 2.60 -10.71 -7.74 5.47 -10.99 -7.28 4.04 2 0 1000.00 -999 ? >ASP.B 40 -9.16 -3.39 -2.15 -11.10 -3.88 -1.48 -10.13 -3.64 -1.81 2 0 1000.00 -999 ? >GLY.B 41 -4.64 -5.14 -3.01 -4.93 -4.81 -2.38 -4.78 -4.98 -2.70 1 0 1000.00 -999 ? >ILE^B 42 -4.43 -2.22 0.67 -3.65 -1.19 0.11 -4.04 -1.71 0.39 3 0 1000.00 -999 ? >PRO^B 43 -0.18 -4.07 3.07 -2.29 -4.73 2.74 -1.23 -4.40 2.90 3 0 1000.00 -999 ? >VAL.B 44 -4.81 -4.09 5.97 -6.99 -4.07 5.87 -5.90 -4.08 5.92 3 0 1000.00 -999 ? >MET?B 45 -2.56 -3.47 8.97 -0.16 -5.39 7.43 -1.36 -4.43 8.20 2 0 1000.00 -999 ? >LEU.B 46 -6.32 -2.14 11.32 -6.23 -0.08 10.63 -6.28 -1.11 10.98 3 0 1000.00 -999 ? >GLU.B 47 -5.25 -5.60 17.41 -4.53 -4.91 19.28 -4.89 -5.26 18.34 2 0 1000.00 -999 ? >SER.B 48 -9.27 -4.11 14.35 -9.03 -1.96 13.23 -9.15 -3.03 13.79 2 0 1000.00 -999 ? >GLU.B 49 -9.48 -3.88 9.60 -11.58 -3.88 9.60 -10.53 -3.88 9.60 2 0 1000.00 -999 ? >ALA.B 50 -6.81 -8.77 11.90 -5.57 -8.58 12.77 -6.19 -8.68 12.33 2 0 1000.00 -999 ? >ARG^B 51 -8.58 -15.61 10.15 -6.05 -17.33 10.31 -7.31 -16.47 10.23 2 0 1000.00 -999 ? >THR^B 52 -6.76 -14.19 15.56 -7.49 -14.03 17.60 -7.13 -14.11 16.58 2 0 1000.00 -999 ? >LEU.B 53 -2.99 -17.17 14.67 -1.67 -16.43 13.15 -2.33 -16.80 13.91 3 0 1000.00 -999 ? >THR~B 54 -6.00 -20.47 15.48 -6.61 -21.44 13.66 -6.31 -20.96 14.57 2 0 1000.00 -999 ? >THR~B 55 -4.31 -22.76 17.95 -4.92 -23.89 19.66 -4.62 -23.33 18.81 2 0 1000.00 -999 ? >GLU~B 57 -3.03 -20.93 11.74 -4.27 -19.35 11.14 -3.65 -20.14 11.44 2 0 1000.00 -999 ? >ARG~B 58 -2.32 -18.88 18.91 -2.92 -16.04 19.90 -2.62 -17.46 19.40 2 0 1000.00 -999 ? >LEU~B 59 1.33 -25.31 17.36 0.03 -26.22 18.83 0.68 -25.76 18.10 3 0 1000.00 -999 ?