REMARK SOURCE hebe.pdb HEADER STRUCTURAL GENOMICS, UNKNOWN FUNCTION 03-JAN-07 2E6U COMPND HYPOTHETICAL PROTEIN PH1109; COMPND 3 CHAIN: X; COMPND 4 ENGINEERED: YES SOURCE MOL_ID: 1; SOURCE 2 ORGANISM_SCIENTIFIC: PYROCOCCUS HORIKOSHII; SOURCE 3 ORGANISM_TAXID: 70601; SOURCE 4 STRAIN: OT3; SOURCE 5 GENE: PH1109; SOURCE 6 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3); SOURCE 7 EXPRESSION_SYSTEM_TAXID: 469008; SOURCE 8 EXPRESSION_SYSTEM_STRAIN: BL21(DE3); SOURCE 9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID; SOURCE 10 EXPRESSION_SYSTEM_PLASMID: PET-26B AUTHOR Y.KITAGO,Y.MIN,N.WATANABE,I.TANAKA HET CA X 201 1 HET CL X 202 1 HET CL X 203 1 HET CL X 204 1 HET COA X 200 48 SITE 1 AC1 5 GLU X 13 GLU X 17 GLU X 92 GLU X 95 SITE 1 AC2 3 TYR X 112 ARG X 114 SER X 117 SITE 1 AC3 2 PRO X 32 LEU X 63 SITE 1 AC4 3 ASP X 51 LYS X 58 GLU X 61 SITE 1 AC5 28 MET X 1 GLU X 2 THR X 20 LYS X 23 >MET.X 1 9.56 49.12 72.87 9.60 50.65 76.07 9.58 49.89 74.47 2 0 -24.19 -999 ? CL CL X 202s >GLU.X 2 11.63 44.53 71.96 13.36 43.32 71.80 12.50 43.92 71.88 2 0 -17.75 -999 ? CL CL X 202s >GLU.X 3 5.15 44.27 74.62 3.30 43.54 73.89 4.22 43.91 74.25 2 0 21.39 -999 ? CL CL X 202 >THR.X 4 7.97 40.86 78.40 7.49 40.72 80.52 7.73 40.79 79.46 2 0 15.81 -999 ? CL CL X 202 >ARG.X 5 7.87 38.21 73.36 5.14 39.34 72.47 6.51 38.77 72.92 2 0 17.78 -999 ? CL CL X 202 >PRO.X 6 7.17 33.79 76.41 8.90 34.84 77.38 8.04 34.31 76.89 3 0 11.09 -999 ? CL CL X 202 >ILE.X 7 12.98 33.07 80.50 13.92 32.64 81.53 13.45 32.86 81.02 3 0 6.07 -999 ? CL CL X 202 >ASP.X 8 11.44 28.70 77.48 9.34 29.09 77.24 10.39 28.89 77.36 2 0 7.71 -999 ? CL CL X 202 >GLY.X 9 11.94 31.80 73.85 11.39 31.26 74.07 11.66 31.53 73.96 1 0 8.49 -999 ? CL CL X 202 >LEU.X 10 8.46 28.44 73.35 9.63 26.59 73.44 9.04 27.51 73.39 3 0 9.82 -999 ? CL CL X 202 >THR~X 11 6.10 31.35 70.73 6.56 31.23 68.61 6.33 31.29 69.67 2 0 15.34 -999 ? CL CL X 202 >ASP~X 12 1.29 31.81 71.43 1.23 33.09 73.14 1.26 32.45 72.28 2 0 15.84 -999 ? S1P COA X 200 >GLU~X 13 2.33 27.64 66.00 2.09 28.10 63.94 2.21 27.87 64.97 2 0 -21.41 -999 ? CL CL X 202s >ASP~X 14 6.72 27.08 70.18 7.98 27.81 68.62 7.35 27.44 69.40 2 0 14.03 -999 ? CL CL X 202 >ILE~X 15 3.15 26.91 74.05 3.94 26.44 75.17 3.55 26.67 74.61 3 0 13.85 -999 ? S1P COA X 200 >ARG~X 16 -2.38 26.51 68.54 -1.71 27.69 65.79 -2.04 27.10 67.17 2 0 21.75 -999 ? S1P COA X 200 >GLU~X 17 2.99 23.60 66.86 4.62 24.31 65.69 3.81 23.96 66.27 2 0 -18.74 -999 ? CL CL X 202s >ILE~X 18 4.46 21.58 73.21 5.64 21.78 73.98 5.05 21.68 73.59 3 0 14.37 -999 ? CL CL X 202 >LEU~X 19 -0.61 22.05 74.27 0.04 23.07 76.20 -0.28 22.56 75.24 3 0 13.14 -999 ? S1P COA X 200 >THR~X 20 -1.49 19.35 70.70 -2.33 20.35 68.97 -1.91 19.85 69.83 2 0 -19.75 -999 ? S1P COA X 200s >ARG~X 21 2.61 14.87 67.79 4.17 12.41 68.84 3.39 13.64 68.32 2 0 22.52 -999 ? CL CL X 202 >TYR~X 22 5.26 16.33 72.21 7.22 16.31 70.21 6.24 16.32 71.21 3 0 17.60 -999 ? CL CL X 202 >LYS.X 23 0.28 14.13 75.28 -4.99 16.06 74.38 -2.35 15.09 74.83 2 0 -17.51 -999 ? S1P COA X 200s >LYS^X 24 2.21 11.26 76.93 0.10 6.41 75.48 1.16 8.83 76.21 2 0 20.60 -999 ? CL CL X 203 >ILE^X 25 2.28 14.09 80.68 2.63 15.32 80.07 2.46 14.70 80.37 3 0 13.47 -999 ? O5P COA X 200 >ALA^X 26 5.43 11.87 83.08 6.84 11.31 82.98 6.14 11.59 83.03 2 0 10.28 -999 ? C2A COA X 200 >LEU^X 27 2.78 14.44 86.13 1.74 16.05 87.28 2.26 15.24 86.70 3 0 9.65 -999 ? N3A COA X 200 >VAL^X 28 7.13 14.22 88.40 9.24 13.97 87.85 8.18 14.10 88.12 3 0 5.07 -999 ? C2A COA X 200 >GLY.X 29 6.21 15.07 91.97 5.71 14.68 91.46 5.96 14.87 91.72 1 0 -3.35 -999 ? N3A COA X 200s >ALA.X 30 2.69 13.76 91.54 2.29 12.56 90.66 2.49 13.16 91.10 2 0 7.04 -999 ? N3A COA X 200 >SER.X 31 0.65 13.89 94.78 0.50 15.15 96.84 0.57 14.52 95.81 2 0 -2.53 -999 ? O9A COA X 200s >PRO.X 32 -1.65 11.30 97.51 -2.08 12.76 95.86 -1.87 12.03 96.68 3 0 -4.69 -999 ? CL CL X 203s >LYS.X 33 -3.10 15.79 98.16 2.01 15.96 102.03 -0.54 15.88 100.09 2 0 -2.73 -999 ? O8A COA X 200s >PRO.X 34 -7.34 16.44 97.85 -5.51 17.47 97.05 -6.42 16.95 97.45 3 0 7.90 -999 ? CL CL X 203 >GLU.X 35 -2.87 20.76 99.91 -3.49 18.96 100.91 -3.18 19.86 100.41 2 0 7.42 -999 ? O8A COA X 200 >ARG~X 36 2.04 19.00 97.10 3.94 20.07 99.22 2.99 19.53 98.16 2 0 -3.03 -999 ? O3A COA X 200s >ASP~X 37 0.46 23.18 92.11 0.02 25.00 93.13 0.24 24.09 92.63 2 0 -3.67 -999 ? N4P COA X 200s >ALA~X 38 0.70 18.74 91.01 1.83 17.82 91.59 1.27 18.28 91.30 2 0 5.89 -999 ? O9P COA X 200 >ASN~X 39 -3.21 17.06 93.37 -4.96 15.84 93.48 -4.08 16.45 93.43 2 0 6.58 -999 ? CL CL X 203 >ILE~X 40 -4.64 21.55 90.86 -5.19 22.74 91.47 -4.92 22.15 91.17 3 0 6.39 -999 ? C2P COA X 200 >VAL~X 41 -2.69 20.08 87.12 -0.91 21.21 86.44 -1.80 20.65 86.78 3 0 5.95 -999 ? C3P COA X 200 >MET~X 42 -2.77 16.23 86.87 -1.48 12.63 88.30 -2.12 14.43 87.59 2 0 8.47 -999 ? CL CL X 203 >LYS~X 43 -6.58 16.23 87.25 -10.19 13.05 87.79 -8.38 14.64 87.52 2 0 9.38 -999 ? CL CL X 203 >TYR~X 44 -6.57 21.25 83.54 -7.12 22.89 81.34 -6.84 22.07 82.44 3 0 8.95 -999 ? S1P COA X 200 >LEU~X 45 -3.14 16.93 82.35 -1.62 18.58 82.06 -2.38 17.75 82.21 3 0 10.70 -999 ? C3P COA X 200 >LEU~X 46 -6.66 13.10 83.77 -5.24 11.56 84.46 -5.95 12.33 84.11 3 0 11.33 -999 ? CL CL X 203 >GLU~X 47 -10.79 16.61 84.05 -10.98 18.37 85.30 -10.88 17.49 84.67 2 0 13.10 -999 ? S1P COA X 200 >HIS~X 48 -8.69 19.59 79.17 -10.06 20.91 80.06 -9.37 20.25 79.62 3 0 13.12 -999 ? S1P COA X 200 >GLY.X 49 -7.06 13.98 76.95 -6.58 14.32 77.46 -6.82 14.15 77.21 1 0 17.73 -999 ? S1P COA X 200 >TYR.X 50 -2.63 16.12 78.40 -2.36 18.49 76.90 -2.50 17.31 77.65 3 0 13.92 -999 ? S1P COA X 200 >ASP^X 51 -1.59 9.35 78.18 -1.61 7.33 78.85 -1.60 8.34 78.51 2 0 -16.81 -999 ? CL CL X 203s >VAL^X 52 -0.56 11.31 82.56 -0.95 13.30 83.42 -0.75 12.30 82.99 3 0 13.27 -999 ? CL CL X 203 >TYR^X 53 2.40 7.29 82.43 1.52 5.62 80.38 1.96 6.45 81.41 3 0 14.94 -999 ? C2A COA X 200 >PRO^X 54 0.61 8.57 88.57 2.12 9.72 87.28 1.37 9.15 87.93 3 0 9.89 -999 ? CL CL X 203 >VAL^X 55 6.08 9.77 89.01 7.74 9.57 87.57 6.91 9.67 88.29 3 0 5.96 -999 ? C2A COA X 200 >ASN.X 56 5.69 10.57 93.40 6.19 11.38 95.31 5.94 10.98 94.36 2 0 -3.23 -999 ? O2B COA X 200s >PRO.X 57 10.69 8.11 93.86 8.52 7.59 94.18 9.61 7.85 94.02 3 0 -4.76 -999 ? N1A COA X 200s >LYS.X 58 6.63 7.74 97.67 4.30 12.17 101.70 5.46 9.96 99.68 2 0 2.85 -999 ? O7A COA X 200s >TYR.X 59 2.27 8.88 96.65 2.00 10.99 98.47 2.13 9.93 97.56 3 0 -4.54 -999 ? O9A COA X 200s >GLU.X 60 2.36 1.03 95.08 4.47 1.37 95.11 3.41 1.20 95.10 2 0 7.62 -999 ? CL CL X 204 >GLU^X 61 -3.24 4.03 91.99 -4.45 2.28 92.31 -3.85 3.15 92.15 2 0 -7.78 -999 ? CL CL X 203s >VAL^X 62 -2.23 8.73 92.41 -0.82 10.39 92.73 -1.52 9.56 92.57 3 0 4.67 -999 ? CL CL X 203 >LEU.X 63 -5.45 11.02 90.26 -5.87 13.06 90.88 -5.66 12.04 90.57 3 0 -5.18 -999 ? CL CL X 203s >GLY.X 64 -6.32 6.13 90.17 -5.64 6.50 89.97 -5.98 6.31 90.07 1 0 6.01 -999 ? CL CL X 203 >ARG^X 65 -3.82 6.91 84.96 -2.16 7.41 82.41 -2.99 7.16 83.69 2 0 13.25 -999 ? CL CL X 203 >LYS^X 66 -0.31 3.61 88.78 2.58 -1.82 87.62 1.13 0.90 88.20 2 0 15.38 -999 ? CL CL X 204 >CYH.X 67 2.52 5.80 90.11 3.37 7.93 91.75 2.94 6.86 90.93 2 0 7.63 -999 ? C2A COA X 200 >TYR.X 68 5.43 4.16 86.78 3.68 2.51 85.37 4.56 3.33 86.07 3 0 12.48 -999 ? C2A COA X 200 >PRO.X 69 9.75 3.20 90.76 8.79 5.23 90.62 9.27 4.22 90.69 3 0 8.70 -999 ? N1A COA X 200 >SER~X 70 11.22 6.97 87.99 12.61 5.34 86.82 11.92 6.15 87.40 2 0 9.75 -999 ? N1A COA X 200 >VAL~X 71 10.50 8.09 84.44 10.59 10.02 83.36 10.54 9.05 83.90 3 0 9.78 -999 ? C2A COA X 200 >LEU~X 72 14.00 4.88 83.46 15.56 5.97 82.40 14.78 5.42 82.93 3 0 10.19 -999 ? CA CA X 201 >ASP~X 73 9.93 2.54 86.49 11.73 1.95 87.44 10.83 2.24 86.96 2 0 12.02 -999 ? N1A COA X 200 >ILE~X 74 6.85 5.18 82.43 6.12 6.40 82.47 6.48 5.79 82.45 3 0 12.80 -999 ? C2A COA X 200 >PRO.X 75 4.48 0.38 81.82 6.21 1.43 80.85 5.35 0.91 81.34 3 0 16.95 -999 ? C2A COA X 200 >ASP.X 76 3.82 4.07 78.00 2.47 4.62 76.39 3.14 4.34 77.20 2 0 19.56 -999 ? C2A COA X 200 >LYS.X 77 6.72 6.12 75.51 4.18 7.01 69.85 5.45 6.57 72.68 2 0 24.25 -999 ? C2A COA X 200 >ILE.X 78 7.64 9.44 78.71 6.57 9.92 79.63 7.11 9.68 79.17 3 0 14.20 -999 ? C2A COA X 200 >GLU.X 79 5.64 11.85 73.46 3.71 11.71 72.59 4.67 11.78 73.03 2 0 20.97 -999 ? O5P COA X 200 >VAL^X 80 6.97 15.39 78.11 5.68 17.15 77.87 6.32 16.27 77.99 3 0 14.22 -999 ? O5P COA X 200 >VAL^X 81 8.52 15.33 81.58 10.20 14.16 82.35 9.36 14.74 81.97 3 0 11.11 -999 ? C2A COA X 200 >ASP^X 82 4.83 17.70 83.41 3.79 19.47 83.96 4.31 18.58 83.69 2 0 7.48 -999 ? O5P COA X 200 >LEU^X 83 9.66 19.39 86.51 11.34 18.21 85.55 10.50 18.80 86.03 3 0 8.72 -999 ? CEP COA X 200 >PHE.X 84 4.20 19.63 89.42 2.22 20.62 87.68 3.21 20.12 88.55 3 0 -4.93 -999 ? C7P COA X 200s >VAL.X 85 9.77 19.04 92.23 10.40 17.08 91.47 10.08 18.06 91.85 3 0 -5.20 -999 ? CEP COA X 200s >LYS~X 86 12.31 21.42 93.71 10.82 21.74 99.50 11.56 21.58 96.61 2 0 -2.88 -999 ? O4A COA X 200s >PRO~X 87 14.44 24.90 92.05 14.58 22.66 91.92 14.51 23.78 91.98 3 0 6.67 -999 ? CEP COA X 200 >LYS~X 88 17.91 20.42 92.63 21.48 23.46 96.89 19.69 21.94 94.76 2 0 13.19 -999 ? O1A COA X 200 >LEU~X 89 14.40 17.05 92.80 14.14 15.71 94.49 14.27 16.38 93.64 3 0 -4.19 -999 ? N6A COA X 200s >THR~X 90 15.16 18.23 88.50 14.61 20.33 88.28 14.89 19.28 88.39 2 0 8.63 -999 ? CEP COA X 200 >MET~X 91 18.74 17.30 87.43 21.41 17.64 85.62 20.07 17.47 86.53 2 0 7.22 -999 ? CA CA X 201 >GLU~X 92 19.33 12.67 90.01 20.75 11.57 88.90 20.04 12.12 89.45 2 0 -2.31 -999 ? CA CA X 201s >TYR~X 93 12.99 13.50 88.05 12.10 11.85 90.17 12.55 12.68 89.11 3 0 -4.54 -999 ? N1A COA X 200s >VAL~X 94 16.19 15.48 83.25 16.76 17.50 82.60 16.47 16.49 82.93 3 0 9.33 -999 ? CA CA X 201 >GLU~X 95 20.95 13.40 84.11 22.41 12.98 85.57 21.68 13.19 84.84 2 0 -2.92 -999 ? CA CA X 201s >GLN~X 96 15.59 9.48 85.60 14.16 9.33 87.19 14.88 9.41 86.39 2 0 7.64 -999 ? N1A COA X 200 >ALA~X 97 14.05 12.17 80.48 13.68 13.66 80.59 13.87 12.92 80.54 2 0 11.35 -999 ? CA CA X 201 >ILE~X 98 18.46 12.68 78.43 19.47 13.59 78.28 18.97 13.14 78.35 3 0 9.92 -999 ? CA CA X 201 >LYS~X 99 17.58 8.31 78.65 21.83 6.48 82.90 19.70 7.40 80.78 2 0 6.17 -999 ? CA CA X 201 >LYS~X 100 13.83 7.74 78.10 9.70 4.39 78.89 11.77 6.06 78.50 2 0 16.27 -999 ? C2A COA X 200 >GLY~X 101 13.88 9.60 74.78 13.43 9.82 75.44 13.66 9.71 75.11 1 0 15.19 -999 ? CA CA X 201 >ALA.X 102 11.42 12.26 75.92 11.20 12.77 77.35 11.31 12.52 76.63 2 0 15.09 -999 ? CA CA X 201 >LYS.X 103 11.05 15.31 73.65 12.34 12.16 69.68 11.69 13.73 71.66 2 0 19.04 -999 ? CL CL X 202 >VAL^X 104 10.19 18.06 76.11 8.45 19.31 75.51 9.32 18.69 75.81 3 0 12.24 -999 ? CL CL X 202 >VAL^X 105 11.24 18.99 79.65 13.10 18.58 80.81 12.17 18.78 80.23 3 0 9.99 -999 ? CL CL X 202 >TRP^X 106 5.99 22.93 82.16 3.89 23.77 78.92 4.94 23.35 80.54 3 0 8.33 -999 ? O5P COA X 200 >PHE^X 107 12.27 23.47 84.18 14.73 22.12 83.81 13.50 22.80 84.00 3 0 7.19 -999 ? CL CL X 202 >GLN.X 108 4.97 22.35 86.30 3.12 22.99 85.39 4.04 22.67 85.85 2 0 -3.93 -999 ? O5P COA X 200s >TYR.X 109 4.55 28.78 88.18 2.50 29.64 86.49 3.52 29.21 87.33 3 0 -3.93 -999 ? O5P COA X 200s >ASN.X 110 8.69 27.39 92.45 7.67 29.27 92.76 8.18 28.33 92.61 2 0 -4.59 -999 ? OAP COA X 200s >THR.X 111 11.25 24.27 89.65 9.61 23.25 88.67 10.43 23.76 89.16 2 0 5.11 -999 ? CEP COA X 200 >TYR.X 112 14.65 27.52 85.61 16.68 28.05 83.78 15.67 27.79 84.69 3 0 -5.01 -999 ? CL CL X 202s >ASN~X 113 17.46 23.14 87.91 19.45 22.48 88.37 18.45 22.81 88.14 2 0 10.52 -999 ? CL CL X 202 >ARG~X 114 21.30 29.58 85.89 22.46 29.81 88.73 21.88 29.70 87.31 2 0 -10.96 -999 ? CL CL X 202s >GLU~X 115 24.30 23.21 86.56 25.75 23.31 84.98 25.02 23.26 85.77 2 0 11.51 -999 ? CL CL X 202 >ALA~X 116 19.28 20.61 84.31 18.06 20.44 85.23 18.67 20.53 84.77 2 0 9.28 -999 ? CL CL X 202 >SER~X 117 18.09 22.91 81.50 17.06 24.85 80.45 17.58 23.88 80.98 2 0 -3.05 -999 ? CL CL X 202s >LYS~X 118 21.69 22.85 80.15 25.13 24.58 76.64 23.41 23.72 78.40 2 0 9.70 -999 ? CL CL X 202 >LYS~X 119 21.73 19.02 79.98 24.59 14.51 83.16 23.16 16.77 81.57 2 0 6.14 -999 ? CA CA X 201 >ALA~X 120 18.32 18.92 78.28 17.01 19.50 78.82 17.66 19.21 78.55 2 0 8.34 -999 ? CL CL X 202 >ASP~X 121 19.81 22.90 76.01 20.09 24.20 74.35 19.95 23.55 75.18 2 0 7.40 -999 ? CL CL X 202 >GLU~X 122 24.48 20.87 76.16 26.31 20.66 77.19 25.39 20.76 76.67 2 0 12.37 -999 ? CL CL X 202 >ALA~X 123 20.69 16.49 74.33 20.19 15.64 75.51 20.44 16.06 74.92 2 0 13.03 -999 ? CA CA X 201 >GLY.X 124 18.28 18.22 71.94 18.02 18.07 72.67 18.15 18.15 72.31 1 0 12.46 -999 ? CL CL X 202 >LEU.X 125 14.93 17.72 75.43 15.29 15.63 75.98 15.11 16.67 75.71 3 0 11.89 -999 ? CL CL X 202 >ILE^X 126 11.35 21.27 73.33 10.39 21.60 72.37 10.87 21.43 72.85 3 0 10.94 -999 ? CL CL X 202 >ILE^X 127 14.10 23.79 77.86 15.20 23.76 78.44 14.65 23.77 78.15 3 0 4.69 -999 ? CL CL X 202 >VAL^X 128 9.63 24.93 78.53 7.54 24.60 77.89 8.59 24.77 78.21 3 0 8.62 -999 ? CL CL X 202 >ALA^X 129 10.46 27.12 81.52 11.91 27.62 81.46 11.18 27.37 81.49 2 0 4.91 -999 ? CL CL X 202 >ASN.X 130 9.16 30.24 84.37 8.56 31.21 86.16 8.86 30.73 85.27 2 0 8.08 -999 ? O5P COA X 200 >ARG.X 131 5.40 28.56 78.81 5.84 30.40 76.36 5.62 29.48 77.59 2 0 11.50 -999 ? CL CL X 202 >CYH~X 132 2.12 25.83 83.78 -0.11 25.07 85.29 1.00 25.45 84.54 2 0 -3.26 -999 ? S1P COA X 200s >MET~X 133 0.73 23.14 81.43 2.53 19.42 80.74 1.63 21.28 81.09 2 0 9.82 -999 ? O5P COA X 200 >MET~X 134 -2.79 23.81 82.73 -5.27 23.62 86.07 -4.03 23.72 84.40 2 0 5.36 -999 ? S1P COA X 200 >ARG~X 135 -3.27 29.60 84.93 -5.52 29.07 87.00 -4.39 29.34 85.97 2 0 -4.60 -999 ? S1P COA X 200s >GLU~X 136 1.59 27.69 79.11 1.58 29.66 78.32 1.58 28.68 78.71 2 0 10.04 -999 ? S1P COA X 200 >HIS~X 137 -4.96 22.72 77.73 -6.50 21.66 76.77 -5.73 22.19 77.25 3 0 12.16 -999 ? S1P COA X 200 >GLU~X 138 -7.21 26.54 81.64 -7.39 27.26 83.65 -7.30 26.90 82.65 2 0 7.12 -999 ? S1P COA X 200 >ARG~X 139 -1.78 32.50 78.04 0.40 32.30 75.86 -0.69 32.40 76.95 2 0 12.93 -999 ? S1P COA X 200 >LEU~X 140 -2.77 28.19 73.84 -0.91 29.35 73.85 -1.84 28.77 73.85 3 0 13.81 -999 ? S1P COA X 200 >LEU~X 141 -6.01 24.45 73.72 -4.59 23.63 72.30 -5.30 24.04 73.01 3 0 15.34 -999 ? S1P COA X 200 >GLY~X 142 -9.51 26.41 76.17 -9.50 26.79 75.45 -9.50 26.60 75.81 1 0 13.96 -999 ? S1P COA X 200