HEADER STRUCTURAL GENOMICS, UNKNOWN FUNCTION 11-AUG-04 1X6J COMPND HYPOTHETICAL PROTEIN YGFY; COMPND 3 CHAIN: A; COMPND 4 ENGINEERED: YES SOURCE MOL_ID: 1; SOURCE 2 ORGANISM_SCIENTIFIC: ESCHERICHIA COLI; SOURCE 3 ORGANISM_TAXID: 562; SOURCE 4 GENE: YGFY; SOURCE 5 EXPRESSION_SYSTEM: ESCHERICHIA COLI; SOURCE 6 EXPRESSION_SYSTEM_TAXID: 562; SOURCE 7 EXPRESSION_SYSTEM_STRAIN: BL21 STAR (DE3); SOURCE 8 EXPRESSION_SYSTEM_VECTOR_TYPE: PET100/D-TOPO AUTHOR K.LIM,V.DOSEEVA,E.SARIKAYA DEMIRKAN,S.PULLALAREVU, AUTHOR 2 W.KRAJEWSKI,A.GALKIN,A.HOWARD,O.HERZBERG,STRUCTURE 2 AUTHOR 3 FUNCTION PROJECT (S2F) mMET.A 1 25.91 19.90 97.10 26.41 19.03 97.18 25.00 21.64 95.53 26.00 21.80 94.82 25.15 21.21 96.99 25.83 22.32 97.82 2 0 1000.00-999 ?-999 ?-999 ? mASP.A 2 23.77 21.78 95.04 23.00 21.56 95.64 22.95 23.56 93.41 22.46 24.18 94.36 23.69 22.25 93.63 23.28 21.17 92.64 2 0 1000.00-999 ?-999 ?-999 ? mILE.A 3 22.87 23.93 92.14 22.87 23.17 91.48 21.39 25.96 91.97 21.31 27.17 92.02 22.77 25.32 91.75 23.32 25.55 90.27 3 0 1000.00-999 ?-999 ?-999 ? mASN.A 4 20.34 25.14 92.13 20.57 24.17 92.15 18.39 25.62 93.64 17.19 25.84 93.79 18.98 25.65 92.24 18.02 24.94 91.29 2 0 1000.00-999 ?-999 ?-999 ? mASN~A 5 19.18 25.30 94.65 20.09 24.95 94.42 19.36 26.69 96.52 20.42 26.59 97.19 18.66 25.49 96.00 18.82 24.27 96.93 2 0 1000.00-999 ?-999 ?-999 ? mLYS~A 6 18.78 27.83 96.17 17.93 27.73 95.65 19.43 29.40 98.00 20.38 30.04 98.43 19.32 29.12 96.50 18.53 30.22 95.82 2 0 1000.00-999 ?-999 ?-999 ? mALA~A 7 18.47 28.90 98.77 17.75 28.40 98.28 19.64 28.38 100.88 20.28 28.94 101.80 18.47 29.09 100.22 17.14 28.67 100.85 2 0 1000.00-999 ?-999 ?-999 ? mARG~A 8 19.95 27.18 100.40 19.33 26.84 99.69 22.43 27.11 100.54 23.35 27.22 101.34 21.11 26.43 100.91 21.09 24.99 100.37 2 0 1000.00-999 ?-999 ?-999 ? mILE~A 9 22.54 27.58 99.31 21.76 27.38 98.71 23.85 29.63 99.70 24.93 30.01 100.12 23.73 28.33 98.90 23.71 28.58 97.35 3 0 1000.00-999 ?-999 ?-999 ? mHIS~A 10 22.74 30.33 99.88 21.95 29.97 99.40 23.32 31.33 102.11 24.16 32.11 102.58 22.69 31.53 100.72 21.24 31.97 100.90 3 0 1000.00-999 ?-999 ?-999 ? mTRP~A 11 22.92 30.24 102.73 22.22 29.72 102.23 24.98 29.61 103.97 25.72 30.12 104.84 23.45 29.82 104.01 22.71 28.56 104.41 3 0 1000.00-999 ?-999 ?-999 ? mALA~A 12 25.44 28.89 102.94 24.72 28.52 102.36 27.69 29.93 102.49 28.91 29.90 102.67 26.86 28.65 102.68 27.01 27.70 101.47 2 0 1000.00-999 ?-999 ?-999 ? mCYH~A 13 27.03 31.04 102.14 26.06 30.87 102.00 27.89 33.13 103.28 28.67 34.10 103.29 27.65 32.37 101.99 26.78 33.26 101.08 2 0 1000.00-999 ?-999 ?-999 ? mARG.A 14 27.25 32.70 104.36 26.56 32.00 104.18 28.86 32.69 106.24 29.00 31.52 106.62 27.54 33.23 105.70 26.38 32.97 106.64 2 0 1000.00-999 ?-999 ?-999 ? mARG.A 15 29.84 33.58 106.30 29.55 34.51 106.08 31.53 33.59 108.10 30.87 34.41 108.74 31.19 33.23 106.65 32.17 33.93 105.69 2 0 1000.00-999 ?-999 ?-999 ? mGLY.A 16 32.56 32.95 108.63 32.97 32.25 108.05 33.61 34.70 110.12 33.68 35.19 111.25 33.00 33.31 109.96-999.99-999.99-999.99 1 0 1000.00-999 ?-999 ?-999 ? mMET~A 17 33.99 35.33 108.99 33.71 34.84 108.17 33.98 37.78 108.32 33.40 37.62 107.25 34.74 36.61 108.95 36.05 36.40 108.18 2 0 1000.00-999 ?-999 ?-999 ? mARG~A 18 34.06 38.96 108.96 34.61 38.91 109.79 33.69 40.63 107.06 32.76 40.91 106.31 33.40 40.20 108.50 33.71 41.40 109.43 2 0 1000.00-999 ?-999 ?-999 ? mGLU~A 19 34.97 40.71 106.69 35.63 40.47 107.41 34.85 40.19 104.27 34.74 40.58 103.10 35.37 41.12 105.35 36.88 41.18 105.24 2 0 1000.00-999 ?-999 ?-999 ? mLEU~A 20 34.59 38.94 104.64 34.79 38.72 105.60 32.54 38.13 103.58 31.96 38.02 102.50 34.06 38.00 103.68 34.47 36.57 104.02 3 0 1000.00-999 ?-999 ?-999 ? mASP~A 21 31.91 38.39 104.70 32.50 38.48 105.51 30.02 39.60 103.80 29.07 39.42 103.04 30.47 38.52 104.72 30.00 38.78 106.14 2 0 1000.00-999 ?-999 ?-999 ? mILE~A 22 30.75 40.70 103.82 31.54 40.62 104.42 30.76 41.75 101.56 30.32 42.56 100.73 30.44 41.90 103.05 31.23 43.07 103.67 3 0 1000.00-999 ?-999 ?-999 ? mSER~A 23 31.53 40.71 101.24 31.80 40.15 102.02 31.09 39.30 99.20 30.54 39.48 98.11 31.93 40.42 99.89 33.38 39.99 99.95 2 0 1000.00-999 ?-999 ?-999 ? mILE~A 24 30.97 38.17 99.88 31.30 38.22 100.82 28.89 36.97 99.28 28.25 36.68 98.29 30.41 36.97 99.27 30.99 35.72 99.97 3 0 1000.00-999 ?-999 ?-999 ? mMET~A 25 28.32 37.26 100.44 28.98 37.38 101.19 26.17 38.30 99.58 25.19 37.87 98.91 26.87 37.39 100.58 26.53 37.83 102.01 2 0 1000.00-999 ?-999 ?-999 ? mPRO~A 26 26.60 39.56 99.50 27.37 39.78 100.09 26.17 40.17 97.10 25.22 40.33 96.31 25.95 40.53 98.58 26.62 41.86 98.94 3 0 1000.00-999 ?-999 ?-999 ? mPHE~A 27 27.36 39.67 96.75 28.06 39.75 97.46 26.50 38.02 95.15 25.85 38.06 94.08 27.59 39.06 95.46 28.99 38.40 95.34 3 0 1000.00-999 ?-999 ?-999 ? mPHE~A 28 26.31 37.06 96.06 26.90 37.12 96.87 23.91 36.64 95.68 23.18 36.33 94.70 25.31 36.03 95.86 25.31 35.00 97.01 3 0 1000.00-999 ?-999 ?-999 ? mGLU~A 29 23.53 37.53 96.59 24.25 37.78 97.24 21.86 38.88 95.33 20.75 38.80 94.76 22.17 38.06 96.59 21.92 38.89 97.84 2 0 1000.00-999 ?-999 ?-999 ? mHIS~A 30 22.86 39.61 94.84 23.72 39.54 95.35 23.14 39.84 92.33 22.67 40.28 91.27 22.67 40.43 93.64 23.35 41.79 93.82 3 0 1000.00-999 ?-999 ?-999 ? mGLU~A 31 24.06 38.86 92.38 24.25 38.50 93.29 24.51 36.95 90.73 24.68 36.61 89.55 24.71 38.41 91.14 26.21 38.70 91.12 2 0 1000.00-999 ?-999 ?-999 ? mTYR~A 32 24.17 36.10 91.70 23.93 36.53 92.57 23.28 34.26 90.26 23.70 33.45 89.40 24.15 34.67 91.46 23.74 33.94 92.73 3 0 1000.00-999 ?-999 ?-999 ? mASP~A 33 22.08 34.84 90.20 21.89 35.50 90.93 21.65 34.77 87.72 21.25 34.11 86.74 21.13 34.49 89.13 19.80 35.19 89.34 2 0 1000.00-999 ?-999 ?-999 ? mSER~A 34 22.58 35.73 87.65 22.90 36.04 88.54 24.22 35.38 85.86 24.66 35.56 84.72 23.10 36.27 86.40 23.63 37.69 86.63 2 0 1000.00-999 ?-999 ?-999 ? mLEU~A 35 24.68 34.45 86.68 24.24 34.46 87.58 25.25 32.41 85.36 24.15 31.91 85.48 25.73 33.52 86.30 26.36 32.88 87.56 3 0 1000.00-999 ?-999 ?-999 ? mSER~A 36 26.12 31.97 84.46 26.93 32.54 84.32 25.74 29.57 84.76 26.14 29.68 85.94 25.86 30.72 83.76 26.98 30.42 82.79 2 0 1000.00-999 ?-999 ?-999 ? mASP~A 37 25.21 28.45 84.30 24.88 28.49 83.36 26.57 26.83 85.53 26.79 26.32 86.62 25.16 27.28 85.15 24.49 26.11 84.42 2 0 1000.00-999 ?-999 ?-999 ? mASP~A 38 27.52 26.98 84.62 27.25 27.41 83.76 29.49 27.37 86.02 30.16 26.87 86.93 28.86 26.51 84.93 29.73 26.51 83.67 2 0 1000.00-999 ?-999 ?-999 ? mGLU~A 39 29.26 28.67 85.92 28.78 28.91 85.07 29.01 29.41 88.31 29.69 29.53 89.32 29.64 29.65 86.92 29.26 31.05 86.40 2 0 1000.00-999 ?-999 ?-999 ? mLYS~A 40 27.74 29.08 88.34 27.28 29.13 87.45 27.74 27.48 90.28 28.03 27.52 91.49 27.07 28.68 89.58 25.59 28.41 89.33 2 0 1000.00-999 ?-999 ?-999 ? mARG~A 41 27.99 26.42 89.51 27.70 26.52 88.56 30.02 25.57 90.60 30.40 25.13 91.66 28.65 25.22 90.03 28.78 24.13 88.94 2 0 1000.00-999 ?-999 ?-999 ? mILE~A 42 30.78 26.38 89.88 30.37 26.66 89.01 32.01 27.61 91.67 32.83 27.47 92.60 32.09 26.78 90.37 32.84 27.57 89.25 3 0 1000.00-999 ?-999 ?-999 ? mPHE~A 43 31.04 28.53 91.70 30.48 28.60 90.87 30.51 28.48 94.06 31.05 28.68 95.13 30.84 29.36 92.86 29.73 30.38 92.61 3 0 1000.00-999 ?-999 ?-999 ? mILE~A 44 29.66 27.47 93.87 29.26 27.40 92.95 30.66 25.91 95.53 30.85 25.80 96.73 29.36 26.55 94.98 28.27 25.52 94.53 3 0 1000.00-999 ?-999 ?-999 ? mARG~A 45 31.55 25.50 94.64 31.29 25.66 93.68 33.71 25.87 95.73 34.38 25.53 96.70 32.78 24.87 95.03 33.42 24.34 93.76 2 0 1000.00-999 ?-999 ?-999 ? mLEU~A 46 33.73 27.11 95.24 33.22 27.24 94.39 34.04 28.24 97.40 34.89 28.39 98.26 34.47 28.15 95.93 34.32 29.50 95.22 3 0 1000.00-999 ?-999 ?-999 ? mLEU~A 47 32.74 28.10 97.67 32.17 27.90 96.87 32.71 27.19 100.00 32.67 27.39 101.19 32.21 28.24 99.01 30.68 28.25 99.01 3 0 1000.00-999 ?-999 ?-999 ? mGLU~A 48 33.18 26.05 99.48 33.04 25.98 98.49 35.29 25.27 100.68 35.82 24.55 101.53 33.83 24.99 100.27 33.83 23.69 99.45 2 0 1000.00-999 ?-999 ?-999 ? mCYH.A 49 35.94 26.26 100.05 35.43 26.66 99.29 37.25 27.31 101.85 36.17 27.77 102.31 37.29 26.69 100.46 37.89 27.68 99.47 2 0 1000.00-999 ?-999 ?-999 ? mASP~A 50 38.39 27.28 102.55 39.16 26.85 102.08 38.51 29.41 103.84 39.02 30.00 102.87 38.47 27.86 103.90 39.72 27.38 104.65 2 0 1000.00-999 ?-999 ?-999 ? mASP~A 51 38.01 30.07 104.87 37.63 29.49 105.59 39.39 32.20 104.75 39.49 33.26 104.07 38.02 31.53 104.90 37.30 32.05 106.15 2 0 1000.00-999 ?-999 ?-999 ? mPRO~A 52 40.44 31.69 105.39 40.25 30.90 105.98 42.17 32.38 103.71 42.72 33.39 103.28 41.77 32.31 105.19 42.70 31.42 105.99 3 0 1000.00-999 ?-999 ?-999 ? mASP~A 53 41.87 31.35 102.94 41.45 30.58 103.42 41.25 32.34 100.73 41.72 33.06 99.85 42.15 31.38 101.51 42.05 29.99 100.96 2 0 1000.00-999 ?-999 ?-999 ? mLEU~A 54 39.94 32.29 101.01 39.70 31.63 101.72 39.37 34.64 100.53 39.26 35.47 99.60 38.99 33.17 100.29 37.52 32.91 100.71 3 0 1000.00-999 ?-999 ?-999 ? mPHE~A 55 39.80 34.96 101.75 39.88 34.21 102.41 41.40 36.79 101.34 41.43 37.90 100.76 40.12 36.34 102.06 40.17 36.61 103.55 3 0 1000.00-999 ?-999 ?-999 ? mASN~A 56 42.41 35.93 101.33 42.29 35.11 101.90 43.27 36.36 99.07 43.76 37.29 98.42 43.61 36.18 100.56 44.65 35.03 100.71 2 0 1000.00-999 ?-999 ?-999 ? mTRP~A 57 42.43 35.48 98.51 42.07 34.78 99.13 41.39 36.94 96.73 41.76 37.65 95.77 42.11 35.62 97.07 41.39 34.40 96.53 3 0 1000.00-999 ?-999 ?-999 ? mLEU~A 58 40.38 37.24 97.55 40.23 36.57 98.28 40.37 39.72 97.60 40.10 40.70 96.92 39.56 38.44 97.39 38.34 38.38 98.31 3 0 1000.00-999 ?-999 ?-999 ? mMET~A 59 41.38 39.68 98.45 41.41 38.83 98.98 43.53 40.73 97.56 44.38 41.61 97.56 42.37 40.77 98.59 42.97 40.73 99.99 2 0 1000.00-999 ?-999 ?-999 ? mASN.A 60 43.56 39.71 96.70 42.79 39.07 96.80 46.00 39.22 96.26 47.02 39.52 95.62 44.61 39.52 95.70 44.63 40.68 94.69 2 0 1000.00-999 ?-999 ?-999 ? mHIS.A 61 46.05 38.66 97.47 45.19 38.67 97.99 47.42 36.74 97.32 47.01 35.70 97.87 47.30 38.10 97.98 47.25 38.00 99.50 3 0 1000.00-999 ?-999 ?-999 ? mGLY.A 62 47.95 36.74 96.10 48.31 37.63 95.81 46.62 35.15 94.76 45.73 35.98 94.62 47.99 35.55 95.29-999.99-999.99-999.99 1 0 1000.00-999 ?-999 ?-999 ? mLYS.A 63 46.43 33.85 94.53 47.15 33.25 94.87 45.18 31.89 93.89 46.21 31.28 94.19 45.24 33.40 93.81 45.33 33.80 92.35 2 0 1000.00-999 ?-999 ?-999 ? mPRO.A 64 44.01 31.29 93.59 43.27 31.92 93.36 44.63 29.22 92.43 44.74 29.78 91.33 43.87 29.84 93.60 42.35 29.62 93.39 3 0 1000.00-999 ?-999 ?-999 ? mALA.A 65 45.17 28.04 92.69 45.08 27.77 93.65 44.82 26.70 90.68 45.15 26.47 89.51 45.83 27.23 91.68 46.52 26.07 92.40 2 0 1000.00-999 ?-999 ?-999 ? mASP~A 66 43.58 26.55 91.15 43.47 26.78 92.11 41.96 27.22 89.45 41.35 28.16 90.00 42.49 26.07 90.31 41.39 25.53 91.22 2 0 1000.00-999 ?-999 ?-999 ? mALA~A 67 42.14 27.18 88.13 42.50 26.32 87.77 40.32 28.70 87.38 39.95 29.86 87.22 41.81 28.35 87.31 42.21 28.15 85.83 2 0 1000.00-999 ?-999 ?-999 ? mGLU~A 68 39.46 27.70 87.58 39.89 26.80 87.65 37.73 28.82 88.90 36.90 29.73 88.87 38.00 27.98 87.67 37.19 26.69 87.71 2 0 1000.00-999 ?-999 ?-999 ? mLEU~A 69 38.39 28.46 89.99 38.98 27.66 89.90 38.85 30.60 91.03 38.26 31.61 91.48 38.25 29.21 91.21 38.93 28.47 92.36 3 0 1000.00-999 ?-999 ?-999 ? mGLU~A 70 40.02 30.69 90.39 40.46 29.82 90.16 39.57 32.89 89.28 39.41 34.04 89.61 40.56 32.02 90.08 41.89 31.96 89.35 2 0 1000.00-999 ?-999 ?-999 ? mMET~A 71 38.95 32.35 88.24 39.20 31.40 88.07 36.78 33.56 88.27 36.27 34.64 88.07 37.99 33.11 87.44 37.49 32.24 86.25 2 0 1000.00-999 ?-999 ?-999 ? mMET~A 72 36.33 32.72 89.20 36.85 31.87 89.28 35.52 34.15 91.00 34.76 35.09 91.19 35.15 33.04 90.01 34.63 31.78 90.74 2 0 1000.00-999 ?-999 ?-999 ? mVAL~A 73 36.70 34.05 91.61 37.23 33.23 91.38 37.15 36.42 91.81 36.67 37.44 92.34 37.14 35.09 92.54 38.53 34.76 93.14 3 0 1000.00-999 ?-999 ?-999 ? mARG~A 74 37.67 36.41 90.59 37.97 35.51 90.28 36.42 38.20 89.41 36.26 39.41 89.34 37.77 37.65 89.81 38.70 37.46 88.60 2 0 1000.00-999 ?-999 ?-999 ? mLEU~A 75 35.46 37.31 89.14 35.74 36.35 89.18 33.42 38.43 89.99 32.76 39.46 89.84 34.11 37.76 88.80 33.26 36.63 88.23 3 0 1000.00-999 ?-999 ?-999 ? mILE~A 76 33.63 37.87 91.17 34.09 36.98 91.14 33.74 39.90 92.57 33.01 40.83 92.91 33.18 38.51 92.41 33.51 37.63 93.64 3 0 1000.00-999 ?-999 ?-999 ? mGLN~A 77 35.03 40.06 92.29 35.50 39.23 92.00 35.13 42.37 91.48 34.89 43.48 91.87 35.69 41.36 92.43 37.20 41.25 92.21 2 0 1000.00-999 ?-999 ?-999 ? mTHR~A 78 35.01 41.97 90.21 35.33 41.04 90.05 33.02 43.23 89.46 32.70 44.43 89.39 34.46 42.81 89.17 34.53 42.03 87.86 2 0 1000.00-999 ?-999 ?-999 ? mARG~A 79 32.15 42.27 89.75 32.52 41.34 89.75 30.60 43.46 91.29 29.77 44.35 91.31 30.76 42.61 90.05 29.89 41.34 90.19 2 0 1000.00-999 ?-999 ?-999 ? mASN~A 80 31.36 43.15 92.33 31.99 42.39 92.17 31.74 45.33 93.43 31.14 46.28 94.00 31.27 43.89 93.60 32.08 43.18 94.69 2 0 1000.00-999 ?-999 ?-999 ? mARG~A 81 32.80 45.47 92.63 33.15 44.61 92.24 32.39 47.70 91.68 32.29 48.90 92.01 33.38 46.79 92.35 34.62 46.64 91.45 2 0 1000.00-999 ?-999 ?-999 ? mGLU~A 82 31.66 47.15 90.72 31.85 46.18 90.56 29.35 48.25 90.72 28.68 49.23 90.40 30.66 47.95 89.97 30.29 47.28 88.64 2 0 1000.00-999 ?-999 ?-999 ? mARG~A 83 29.00 47.42 91.69 29.67 46.70 91.86 27.88 48.47 93.64 26.92 49.19 94.01 27.76 47.55 92.43 27.31 46.16 92.90 2 0 1000.00-999 ?-999 ?-999 ? mGLY~A 84 29.07 48.45 94.24 29.78 47.96 93.74 29.35 50.62 95.42 29.08 51.19 94.35 29.32 49.10 95.51-999.99-999.99-999.99 1 0 1000.00-999 ?-999 ?-999 ? mPRO.A 85 29.62 51.24 96.57 29.74 50.61 97.33 30.87 53.37 95.93 32.04 53.01 96.10 29.73 52.71 96.71 29.97 52.89 98.22 3 0 1000.00-999 ?-999 ?-999 ? mVAL.A 86 30.51 54.34 95.10 29.53 54.47 95.02 31.92 56.29 95.32 31.11 57.18 95.64 31.50 55.15 94.39 30.95 55.70 93.05 3 0 1000.00-999 ?-999 ?-999 ? mALA.A 87 33.19 56.26 95.75 33.76 55.59 95.28 34.58 58.28 96.35 35.68 58.03 95.86 33.67 57.13 96.83 34.39 56.27 97.87 2 0 1000.00-999 ?-999 ?-999 ? mILE.A 88 34.14 59.54 96.51 33.19 59.61 96.83 36.23 60.83 97.16 36.31 60.22 98.25 35.02 60.69 96.20 34.26 62.06 96.11 3 0 1000.00-999 ?-999 ?-999 ?