REMARK SOURCE hebe.pdb HEADER STRUCTURAL GENOMICS, UNKNOWN FUNCTION 19-FEB-02 1L1S COMPND HYPOTHETICAL PROTEIN MTH1491; COMPND 3 CHAIN: A; COMPND 4 ENGINEERED: YES SOURCE MOL_ID: 1; SOURCE 2 ORGANISM_SCIENTIFIC: METHANOTHERMOBACTER THERMAUTOTROPHICUS; SOURCE 3 ORGANISM_TAXID: 145262; SOURCE 4 GENE: ORF1491; SOURCE 5 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3); SOURCE 6 EXPRESSION_SYSTEM_TAXID: 469008; SOURCE 7 EXPRESSION_SYSTEM_STRAIN: BL21(DE3); SOURCE 8 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID; SOURCE 9 EXPRESSION_SYSTEM_PLASMID: PET15B AUTHOR D.CHRISTENDAT,V.SARIDAKIS,Y.KIM,P.A.KUMAR,X.XU,A.SEMESI,A.JOACHIMIAK, AUTHOR 2 C.H.ARROWSMITH,A.M.EDWARDS,MIDWEST CENTER FOR STRUCTURAL GENOMICS AUTHOR 3 (MCSG) HET MSE A 29 8 HET MSE A 44 8 HET MSE A 81 8 >ASP.A 3 12.62 56.94 47.90 11.19 58.39 48.42 11.90 57.67 48.16 2 0 1000.00 -999 ? >TYR^A 4 9.30 56.81 44.60 7.68 58.87 45.58 8.49 57.84 45.09 3 0 1000.00 -999 ? >ARG^A 5 11.67 49.26 46.72 9.41 47.94 48.29 10.54 48.60 47.50 2 0 1000.00 -999 ? >VAL^A 6 9.61 50.53 40.05 9.49 52.14 38.56 9.55 51.33 39.31 3 0 1000.00 -999 ? >VAL^A 7 9.81 47.73 37.53 9.07 45.67 37.70 9.44 46.70 37.61 3 0 1000.00 -999 ? >PHE^A 8 9.89 50.66 33.29 9.78 53.39 33.93 9.84 52.03 33.61 3 0 1000.00 -999 ? >HIS^A 9 5.68 44.08 31.27 4.58 42.62 30.24 5.13 43.35 30.75 3 0 1000.00 -999 ? >ILE^A 10 9.54 46.26 27.38 10.65 46.58 26.53 10.09 46.42 26.95 3 0 1000.00 -999 ? >ASP.A 11 6.47 41.48 26.19 4.54 42.40 26.14 5.50 41.94 26.17 2 0 1000.00 -999 ? >GLU.A 12 5.34 44.38 22.30 3.82 45.86 22.17 4.58 45.12 22.23 2 0 1000.00 -999 ? >ASP.A 13 11.92 44.22 19.88 11.48 43.15 18.10 11.70 43.68 18.99 2 0 1000.00 -999 ? >ASP~A 14 7.75 47.88 17.53 6.68 48.71 19.15 7.22 48.30 18.34 2 0 1000.00 -999 ? >GLU~A 15 12.23 53.06 16.68 13.78 53.57 15.31 13.00 53.32 16.00 2 0 1000.00 -999 ? >SER~A 16 8.63 53.42 19.14 6.68 52.63 17.93 7.65 53.03 18.53 2 0 1000.00 -999 ? >ARG~A 17 5.31 49.11 23.52 2.97 50.91 22.67 4.14 50.01 23.09 2 0 1000.00 -999 ? >VAL~A 18 11.55 50.69 22.95 13.03 49.26 22.22 12.29 49.97 22.59 3 0 1000.00 -999 ? >LEU~A 19 12.35 55.24 21.45 14.05 55.33 20.06 13.20 55.29 20.76 3 0 1000.00 -999 ? >LEU~A 20 7.87 54.72 24.61 6.27 56.05 25.20 7.07 55.38 24.90 3 0 1000.00 -999 ? >LEU~A 21 10.94 51.31 27.45 11.73 49.86 28.87 11.34 50.58 28.16 3 0 1000.00 -999 ? >ILE~A 22 14.94 54.42 26.19 16.12 54.15 25.44 15.53 54.28 25.82 3 0 1000.00 -999 ? >SER~A 23 12.59 57.82 27.59 10.94 59.52 27.10 11.76 58.67 27.34 2 0 1000.00 -999 ? >ASN~A 24 9.27 55.76 30.49 7.61 56.19 29.22 8.44 55.98 29.85 2 0 1000.00 -999 ? >VAL~A 25 13.33 55.43 32.44 14.55 53.62 32.45 13.94 54.53 32.45 3 0 1000.00 -999 ? >ARG~A 26 17.61 58.83 28.87 19.15 57.38 26.63 18.38 58.11 27.75 2 0 1000.00 -999 ? >ASN~A 27 10.99 60.74 32.00 9.54 62.17 32.69 10.26 61.46 32.34 2 0 1000.00 -999 ? >LEU~A 28 11.41 57.44 35.94 10.05 56.51 37.32 10.73 56.98 36.63 3 0 1000.00 -999 ? >MET?A 29 15.29 59.30 37.17 18.21 56.59 35.61 16.75 57.95 36.39 2 0 1000.00 -999 ? >ALA~A 30 15.04 63.08 36.72 15.11 63.73 35.35 15.07 63.40 36.04 2 0 1000.00 -999 ? >ASP~A 31 10.25 63.26 37.50 8.58 64.34 38.27 9.42 63.80 37.89 2 0 1000.00 -999 ? >LEU~A 32 11.65 59.97 41.30 9.62 59.43 40.78 10.64 59.70 41.04 3 0 1000.00 -999 ? >GLU.A 33 13.58 64.05 45.56 12.79 64.23 47.53 13.18 64.14 46.54 2 0 1000.00 -999 ? >SER.A 34 15.34 58.67 44.81 15.85 57.11 46.59 15.59 57.89 45.70 2 0 1000.00 -999 ? >VAL^A 35 14.24 55.70 42.71 13.37 55.82 40.73 13.81 55.76 41.72 3 0 1000.00 -999 ? >ARG^A 36 16.19 49.13 44.39 16.71 46.30 45.41 16.45 47.72 44.90 2 0 1000.00 -999 ? >ILE^A 37 14.64 51.90 37.51 14.06 52.90 36.58 14.35 52.40 37.05 3 0 1000.00 -999 ? >GLU^A 38 14.90 46.16 39.14 14.73 44.29 40.11 14.82 45.23 39.62 2 0 1000.00 -999 ? >VAL^A 39 13.45 46.70 33.41 13.84 48.12 31.80 13.65 47.41 32.60 3 0 1000.00 -999 ? >VAL^A 40 12.01 43.26 32.70 11.09 41.93 34.16 11.55 42.60 33.43 3 0 1000.00 -999 ? >ALA^A 41 12.00 42.27 29.05 13.08 42.89 28.19 12.54 42.58 28.62 2 0 1000.00 -999 ? >TYR.A 42 8.43 38.63 29.04 5.78 39.35 29.65 7.10 38.99 29.34 3 0 1000.00 -999 ? >SER~A 43 9.41 37.53 24.40 7.47 37.75 22.95 8.44 37.64 23.68 2 0 1000.00 -999 ? >MET?A 44 10.41 39.81 21.51 10.13 38.27 18.73 10.27 39.04 20.12 2 0 1000.00 -999 ? >GLY~A 45 11.39 42.35 24.14 11.77 41.67 23.94 11.58 42.01 24.04 1 0 1000.00 -999 ? >VAL~A 46 14.58 40.48 24.98 14.20 38.39 25.34 14.39 39.43 25.16 3 0 1000.00 -999 ? >ASN~A 47 15.28 41.43 20.23 14.53 39.56 19.55 14.90 40.50 19.89 2 0 1000.00 -999 ? >VAL~A 48 16.55 44.78 23.10 15.31 46.46 23.70 15.93 45.62 23.40 3 0 1000.00 -999 ? >LEU~A 49 18.52 42.56 26.83 17.28 43.49 28.35 17.90 43.03 27.59 3 0 1000.00 -999 ? >ARG~A 50 19.35 39.81 19.91 20.44 37.00 19.32 19.89 38.40 19.61 2 0 1000.00 -999 ? >ARG.A 51 28.57 41.27 22.39 28.02 38.82 24.15 28.30 40.04 23.27 2 0 1000.00 -999 ? >ASP.A 52 24.16 42.06 17.71 22.47 40.76 17.86 23.32 41.41 17.79 2 0 1000.00 -999 ? >SER.A 53 21.18 45.37 19.54 19.29 46.22 20.81 20.24 45.79 20.17 2 0 1000.00 -999 ? >GLU.A 54 18.47 49.01 17.55 17.05 49.10 15.98 17.76 49.05 16.76 2 0 1000.00 -999 ? >TYR~A 55 17.40 49.64 21.22 15.24 49.78 19.43 16.32 49.71 20.33 3 0 1000.00 -999 ? >SER~A 56 23.08 48.56 23.30 24.50 48.30 21.34 23.79 48.43 22.32 2 0 1000.00 -999 ? >GLY~A 57 24.46 52.04 23.87 23.72 51.69 24.12 24.09 51.87 24.00 1 0 1000.00 -999 ? >ASP~A 58 19.88 53.20 25.02 19.70 54.90 23.77 19.79 54.05 24.40 2 0 1000.00 -999 ? >VAL~A 59 21.16 49.85 27.66 20.32 47.86 27.40 20.74 48.85 27.53 3 0 1000.00 -999 ? >SER~A 60 24.81 49.94 28.75 26.36 51.06 27.26 25.59 50.50 28.01 2 0 1000.00 -999 ? >GLU~A 61 24.01 56.06 29.53 23.57 57.67 28.25 23.79 56.87 28.89 2 0 1000.00 -999 ? >LEU~A 62 20.04 52.90 31.39 18.62 54.37 30.70 19.33 53.63 31.05 3 0 1000.00 -999 ? >THR~A 63 22.99 50.15 33.51 23.57 48.14 32.94 23.28 49.14 33.23 2 0 1000.00 -999 ? >GLY~A 64 25.86 52.37 34.59 25.04 52.50 34.69 25.45 52.44 34.64 1 0 1000.00 -999 ? >GLN~A 65 23.25 56.51 34.34 22.09 57.57 32.92 22.67 57.04 33.63 2 0 1000.00 -999 ? >GLY.A 66 21.94 51.96 38.31 21.68 51.95 37.57 21.81 51.95 37.94 1 0 1000.00 -999 ? >VAL.A 67 19.19 50.60 36.06 17.90 51.56 34.56 18.55 51.08 35.31 3 0 1000.00 -999 ? >ARG^A 68 18.47 43.91 38.75 17.86 41.09 39.79 18.16 42.50 39.27 2 0 1000.00 -999 ? >PHE^A 69 18.25 46.83 31.19 17.94 49.49 30.43 18.09 48.16 30.81 3 0 1000.00 -999 ? >CYH^A 70 16.48 41.55 33.42 16.05 42.36 36.07 16.27 41.96 34.75 2 0 1000.00 -999 ? >ALA^A 71 16.09 38.89 30.73 17.27 38.50 29.85 16.68 38.69 30.29 2 0 1000.00 -999 ? >CYH^A 72 13.73 35.94 31.11 11.27 34.64 31.15 12.50 35.29 31.13 2 0 1000.00 -999 ? >SER~A 73 15.52 32.62 31.67 16.49 30.78 32.99 16.00 31.70 32.33 2 0 1000.00 -999 ? >ASN~A 74 11.25 30.71 30.43 11.26 29.89 32.41 11.26 30.30 31.42 2 0 1000.00 -999 ? >THR~A 75 12.06 33.03 26.87 11.65 35.06 27.56 11.86 34.05 27.22 2 0 1000.00 -999 ? >LEU~A 76 16.93 32.97 27.24 18.03 34.74 27.75 17.48 33.85 27.50 3 0 1000.00 -999 ? >ARG~A 77 14.90 25.77 27.11 14.43 22.81 27.75 14.66 24.29 27.43 2 0 1000.00 -999 ? >ALA~A 78 12.47 29.49 23.65 11.22 30.16 24.23 11.85 29.83 23.94 2 0 1000.00 -999 ? >SER~A 79 14.28 32.04 21.49 13.01 33.96 22.26 13.65 33.00 21.87 2 0 1000.00 -999 ? >GLY.A 80 17.47 30.05 20.98 17.31 30.78 21.39 17.39 30.41 21.19 1 0 1000.00 -999 ? >MET?A 81 19.43 32.39 23.24 17.93 36.41 22.73 18.68 34.40 22.98 2 0 1000.00 -999 ? >ASP~A 82 22.64 30.82 26.17 24.61 31.43 25.71 23.62 31.13 25.94 2 0 1000.00 -999 ? >GLY~A 83 23.41 34.47 28.58 23.68 34.18 27.92 23.54 34.32 28.25 1 0 1000.00 -999 ? >ASP~A 84 27.06 33.52 25.54 28.57 32.70 26.78 27.82 33.11 26.16 2 0 1000.00 -999 ? >ASP~A 85 22.83 35.88 23.05 23.20 33.95 22.25 23.02 34.91 22.65 2 0 1000.00 -999 ? >LEU~A 86 22.50 38.99 27.52 20.57 38.08 27.80 21.53 38.54 27.66 3 0 1000.00 -999 ? >LEU.A 87 24.24 43.96 26.37 25.06 45.87 25.81 24.65 44.91 26.09 3 0 1000.00 -999 ? >GLU.A 88 28.89 44.74 29.12 30.91 45.41 29.33 29.90 45.07 29.23 2 0 1000.00 -999 ? >GLY.A 89 25.46 44.14 32.73 25.11 43.51 32.31 25.28 43.83 32.52 1 0 1000.00 -999 ? >VAL.A 90 22.15 42.55 31.80 21.01 42.55 29.94 21.58 42.55 30.87 3 0 1000.00 -999 ? >ASP^A 91 20.64 40.54 35.87 22.12 41.78 36.73 21.38 41.16 36.30 2 0 1000.00 -999 ? >VAL^A 92 18.91 36.98 34.02 19.49 35.39 32.65 19.20 36.18 33.33 3 0 1000.00 -999 ? >VAL^A 93 15.89 36.17 36.16 14.71 38.01 36.11 15.30 37.09 36.13 3 0 1000.00 -999 ? >SER.A 94 14.24 32.77 36.28 13.21 32.76 38.49 13.73 32.77 37.39 2 0 1000.00 -999 ? >SER~A 95 10.97 34.18 34.88 8.57 33.96 35.05 9.77 34.07 34.96 2 0 1000.00 -999 ? >GLY~A 96 10.05 37.58 33.48 9.59 37.06 33.94 9.82 37.32 33.71 1 0 1000.00 -999 ? >VAL~A 97 6.44 37.54 34.65 5.22 36.40 33.25 5.83 36.97 33.95 3 0 1000.00 -999 ? >GLY~A 98 7.37 35.77 37.87 7.67 36.49 37.55 7.52 36.13 37.71 1 0 1000.00 -999 ? >HIS~A 99 12.13 39.74 38.58 13.39 40.97 39.74 12.76 40.35 39.16 3 0 1000.00 -999 ? >ILE~A 100 6.66 41.36 36.67 6.28 42.20 35.51 6.47 41.78 36.09 3 0 1000.00 -999 ? >VAL~A 101 4.86 39.44 40.27 3.30 37.91 40.08 4.08 38.68 40.17 3 0 1000.00 -999 ? >ARG~A 102 9.98 36.08 43.05 9.97 33.54 41.33 9.98 34.81 42.19 2 0 1000.00 -999 ? >ARG~A 103 11.60 43.40 41.59 13.96 42.43 43.33 12.78 42.91 42.46 2 0 1000.00 -999 ? >GLN~A 104 3.80 44.44 40.51 2.15 44.10 39.19 2.97 44.27 39.85 2 0 1000.00 -999 ? >THR~A 105 4.26 41.68 45.76 3.77 39.60 45.92 4.01 40.64 45.84 2 0 1000.00 -999 ? >GLU~A 106 9.18 41.58 47.22 10.79 41.09 45.92 9.99 41.33 46.57 2 0 1000.00 -999 ? >GLY.A 107 5.72 46.74 47.08 6.10 46.46 46.45 5.91 46.60 46.77 1 0 1000.00 -999 ? >TRP.A 108 9.78 46.07 44.81 12.99 46.97 42.70 11.39 46.52 43.75 3 0 1000.00 -999 ? >ALA^A 109 5.28 49.99 41.40 5.41 51.49 41.29 5.34 50.74 41.35 2 0 1000.00 -999 ? >TYR^A 110 3.39 46.71 36.62 2.80 45.25 34.27 3.09 45.98 35.44 3 0 1000.00 -999 ? >ILE^A 111 5.80 51.65 34.51 4.91 52.80 34.62 5.35 52.23 34.57 3 0 1000.00 -999 ? >ARG.A 112 2.53 47.24 28.81 2.39 46.30 25.89 2.46 46.77 27.35 2 0 1000.00 -999 ? >PRO.A 113 6.96 51.57 29.58 4.89 50.99 29.01 5.93 51.28 29.29 3 0 1000.00 -999 ?