SIMILAR PATTERNS OF AMINO ACIDS FOR 5U6N_A_SALA505_1

List of similar pattern of amino acids derived from ASSAM search

Hit pattern: 3D amino acid arrangements similar to known drug binding site
Query pattern: Residues from known binding site for annotated drug that match the hit pattern

(Click link on the last column to view superposed patterns of amino acids in NGL viewer)
(Note that only 3-12 residue patterns can be used for ASSAM searches)
Filter list by:
Hit Macromolecule/
Organism		 Pfam Res. Interface HETATM RMSD Dali Z-score Seq. Identity (%) View Dock
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
1onk GALACTOSE SPECIFIC
LECTIN I B CHAIN

(Viscum album) 		PF00652
(Ricin_B_lectin) 		4 SER B 104
GLN B 130
TRP B  94
THR B  98
 None
None
NAG  B5002 (-4.2A)
None
 1.42A 5u6nA-1onkB:
undetectable		 5u6nA-1onkB:
18.79
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
1qwk ALDO-KETO REDUCTASE
FAMILY 1 MEMBER C1

(Caenorhabditis
elegans) 		PF00248
(Aldo_ket_red) 		4 SER A 197
GLN A 175
MET A  16
THR A  79
 None
 1.30A 5u6nA-1qwkA:
2.3		 5u6nA-1qwkA:
21.76
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
1sjj ACTININ

(Gallus gallus) 		PF00307
(CH)
PF00435
(Spectrin)
PF08726
(EFhand_Ca_insen) 		4 SER A 173
GLN A 156
MET A 355
THR A 159
 None
 1.20A 5u6nA-1sjjA:
undetectable		 5u6nA-1sjjA:
19.93
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
1tp7 GENOME POLYPROTEIN

(Rhinovirus A) 		PF00680
(RdRP_1) 		4 SER A 270
PHE A 247
MET A 154
THR A 292
 None
 1.18A 5u6nA-1tp7A:
undetectable		 5u6nA-1tp7A:
22.84
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
2bm7 PENTAPEPTIDE REPEAT
FAMILY PROTEIN

(Mycobacterium
tuberculosis) 		PF13599
(Pentapeptide_4) 		4 SER A  42
HIS A  44
PHE A  49
TRP A   4
 None
 1.47A 5u6nA-2bm7A:
undetectable		 5u6nA-2bm7A:
18.20
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
2btw ALR0975 PROTEIN

(Nostoc sp. PCC
7120) 		PF05023
(Phytochelatin) 		4 PHE A 236
GLN A 155
MET A 221
THR A 147
 None
 1.20A 5u6nA-2btwA:
undetectable		 5u6nA-2btwA:
20.92
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
2f6r BIFUNCTIONAL
COENZYME A SYNTHASE

(Mus musculus) 		PF01121
(CoaE) 		4 SER A 222
MET A 178
TRP A 150
THR A 146
 ACO  A 270 ( 3.9A)
ACO  A 270 (-3.9A)
ACO  A 270 (-3.9A)
ACO  A 270 (-4.6A)
 1.26A 5u6nA-2f6rA:
4.3		 5u6nA-2f6rA:
22.27
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
2g8y MALATE/L-LACTATE
DEHYDROGENASES

(Escherichia
coli) 		PF02615
(Ldh_2) 		4 SER A  47
PHE A 188
GLN A 221
MET A 149
 SO4  A1007 (-2.9A)
None
None
EDO  A1019 (-3.8A)
 1.46A 5u6nA-2g8yA:
undetectable		 5u6nA-2g8yA:
20.35
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
2nlz CEPHALOSPORIN
ACYLASE

(Bacillus
halodurans) 		PF01019
(G_glu_transpept) 		4 SER A  65
GLN A  30
MET A 439
THR A 532
 None
 1.47A 5u6nA-2nlzA:
undetectable		 5u6nA-2nlzA:
20.78
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
2nox TRYPTOPHAN
2,3-DIOXYGENASE

(Cupriavidus
metallidurans) 		PF03301
(Trp_dioxygenase) 		4 HIS A  72
PHE A  68
MET A 133
THR A 271
 HEM  A 500 (-3.5A)
HEM  A 500 (-4.4A)
None
None
 1.27A 5u6nA-2noxA:
undetectable		 5u6nA-2noxA:
21.92
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
2oi8 PUTATIVE REGULATORY
PROTEIN SCO4313

(Streptomyces
coelicolor) 		PF00440
(TetR_N) 		4 HIS A 184
PHE A 111
GLN A  75
THR A 131
 None
 1.22A 5u6nA-2oi8A:
undetectable		 5u6nA-2oi8A:
19.77
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
2otb GFP-LIKE FLUORESCENT
CHROMOPROTEIN CFP484

(Clavularia sp.) 		PF01353
(GFP) 		4 SER A 146
HIS A 163
PHE A 177
THR A  62
 PIA  A  66 ( 2.7A)
PIA  A  66 ( 3.6A)
None
None
 1.23A 5u6nA-2otbA:
undetectable		 5u6nA-2otbA:
19.22
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
2q6z UROPORPHYRINOGEN
DECARBOXYLASE

(Homo sapiens) 		PF01208
(URO-D) 		4 SER A  85
PHE A  56
GLN A  92
MET A 100
 None
 1.43A 5u6nA-2q6zA:
3.6		 5u6nA-2q6zA:
22.48
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
2qri H-2 CLASS I
HISTOCOMPATIBILITY
ANTIGEN K-B ALPHA
CHAIN, BETA-2
MICROGLOBULIN,
OVALBUMIN-DERIVED
PEPTIDE

(Mus musculus) 		PF00129
(MHC_I)
PF07654
(C1-set) 		4 HIS A  31
PHE A  62
GLN A  96
MET A  54
 None
 1.19A 5u6nA-2qriA:
undetectable		 5u6nA-2qriA:
19.43
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
2qty POLY(ADP-RIBOSE)
GLYCOHYDROLASE ARH3

(Mus musculus) 		PF03747
(ADP_ribosyl_GH) 		4 SER A 132
HIS A 166
MET A  65
THR A 301
 None
None
None
MG  A 349 (-3.4A)
 1.48A 5u6nA-2qtyA:
undetectable		 5u6nA-2qtyA:
21.49
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
2rcq RETINOL-BINDING
PROTEIN II, CELLULAR

(Homo sapiens) 		PF00061
(Lipocalin) 		4 SER A  55
PHE A  16
GLN A 128
MET A  20
 None
 1.40A 5u6nA-2rcqA:
undetectable		 5u6nA-2rcqA:
14.80
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
2wj3 1-H-3-HYDROXY-4-OXOQ
UINALDINE
2,4-DIOXYGENASE

(Paenarthrobacter
nitroguajacolicus) 		PF00561
(Abhydrolase_1) 		4 SER A 101
HIS A 100
PHE A 252
TRP A 185
 None
 1.42A 5u6nA-2wj3A:
2.6		 5u6nA-2wj3A:
18.49
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
2xhe SYNTAXIN1
UNC18

(Monosiga
brevicollis) 		PF00804
(Syntaxin)
PF00995
(Sec1)
PF05739
(SNARE) 		4 PHE B  46
GLN B 139
MET B  43
THR A  52
 None
 1.46A 5u6nA-2xheB:
undetectable		 5u6nA-2xheB:
20.84
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
2xmo LMO2642 PROTEIN

(Listeria
monocytogenes) 		PF00149
(Metallophos) 		4 HIS A 311
PHE A 351
GLN A  73
THR A 358
 None
 1.24A 5u6nA-2xmoA:
undetectable		 5u6nA-2xmoA:
22.81
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
3ay2 LIPID MODIFIED
AZURIN PROTEIN

(Neisseria
gonorrhoeae) 		PF00127
(Copper-bind) 		4 SER A  65
HIS A  91
GLN A  95
MET A 175
 None
None
None
ZN  A1001 (-3.5A)
 1.40A 5u6nA-3ay2A:
undetectable		 5u6nA-3ay2A:
15.99
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
3dra GERANYLGERANYLTRANSF
ERASE TYPE I BETA
SUBUNIT
PROTEIN
FARNESYLTRANSFERASE/
GERANYLGERANYLTRANSF
ERASE TYPE-1 SUBUNIT
ALPHA

(Candida
albicans) 		PF00432
(Prenyltrans)
PF01239
(PPTA) 		4 SER B  78
HIS A  66
MET A  36
THR B  85
 None
 1.37A 5u6nA-3draB:
undetectable		 5u6nA-3draB:
21.96
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
3e1t HALOGENASE

(Chondromyces
crocatus) 		PF04820
(Trp_halogenase) 		4 SER A  20
PHE A 113
MET A  68
THR A  54
 None
 1.30A 5u6nA-3e1tA:
undetectable		 5u6nA-3e1tA:
21.07
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
3flo DNA POLYMERASE ALPHA
SUBUNIT B

(Saccharomyces
cerevisiae) 		PF04042
(DNA_pol_E_B)
PF08418
(Pol_alpha_B_N) 		4 SER A 341
HIS A 563
PHE A 343
GLN A 567
 SO4  A   3 ( 4.5A)
SO4  A   3 (-3.5A)
None
None
 1.47A 5u6nA-3floA:
undetectable		 5u6nA-3floA:
21.10
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
3hs0 COBRA VENOM FACTOR

(Naja kaouthia) 		PF00207
(A2M)
PF01759
(NTR)
PF07677
(A2M_recep) 		4 SER B 838
PHE B 860
GLN B 846
THR C1366
 None
 1.22A 5u6nA-3hs0B:
undetectable		 5u6nA-3hs0B:
20.70
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
3i04 CARBON MONOXIDE
DEHYDROGENASE/ACETYL
-COA SYNTHASE
SUBUNIT BETA

(Moorella
thermoacetica) 		PF03063
(Prismane) 		4 PHE A  49
GLN A 477
MET A 357
THR A 332
 None
 1.30A 5u6nA-3i04A:
6.0		 5u6nA-3i04A:
21.34
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
3mi6 ALPHA-GALACTOSIDASE

(Lactobacillus
brevis) 		PF02065
(Melibiase)
PF16874
(Glyco_hydro_36C)
PF16875
(Glyco_hydro_36N) 		4 SER A 653
PHE A 732
GLN A 730
THR A 680
 None
 1.37A 5u6nA-3mi6A:
2.7		 5u6nA-3mi6A:
21.03
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
3phg SHIKIMATE
DEHYDROGENASE

(Helicobacter
pylori) 		PF08501
(Shikimate_dh_N) 		4 SER A  18
HIS A  22
PHE A 245
THR A 104
 None
 1.38A 5u6nA-3phgA:
4.7		 5u6nA-3phgA:
21.59
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
3rko NADH-QUINONE
OXIDOREDUCTASE
SUBUNIT L

(Escherichia
coli) 		no annotation 4 SER L 398
HIS L 334
GLN L 315
THR L 425
 None
 1.14A 5u6nA-3rkoL:
undetectable		 5u6nA-3rkoL:
21.61
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
3rwk INULINASE

(Aspergillus
ficuum) 		PF00251
(Glyco_hydro_32N)
PF08244
(Glyco_hydro_32C) 		4 SER X 201
HIS X 194
MET X 190
THR X 126
 None
 1.28A 5u6nA-3rwkX:
undetectable		 5u6nA-3rwkX:
22.75
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
3v8x TRANSFERRIN-BINDING
PROTEIN 1

(Neisseria
meningitidis) 		PF00593
(TonB_dep_Rec)
PF07715
(Plug) 		4 SER A 137
HIS A 403
PHE A 338
GLN A 336
 None
 1.36A 5u6nA-3v8xA:
undetectable		 5u6nA-3v8xA:
19.41
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
4ars HISTIDINE ACID
PHOSPHATASE

(Hafnia alvei) 		PF00328
(His_Phos_2) 		4 PHE A 224
MET A 168
MET A  27
THR A  35
 None
 1.30A 5u6nA-4arsA:
undetectable		 5u6nA-4arsA:
21.55
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
4p4g SHIKIMATE
5-DEHYDROGENASE AROE
(5-DEHYDROSHIKIMATE
REDUCTASE)

(Mycobacterium
tuberculosis) 		PF08501
(Shikimate_dh_N) 		4 SER A  20
HIS A  24
PHE A 251
THR A 104
 SKM  A 301 (-2.6A)
None
None
None
 1.41A 5u6nA-4p4gA:
2.1		 5u6nA-4p4gA:
19.87
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
4yv2 SPERMIDINE SYNTHASE,
PUTATIVE

(Trypanosoma
cruzi) 		PF01564
(Spermine_synth)
PF17284
(Spermine_synt_N) 		4 SER A  84
HIS A 279
PHE A 283
THR A 244
 None
 1.26A 5u6nA-4yv2A:
3.2		 5u6nA-4yv2A:
19.82
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
5coa IRIDOID SYNTHASE

(Catharanthus
roseus) 		no annotation 4 PHE A 207
MET A 381
TRP A 320
THR A 332
 None
 1.41A 5u6nA-5coaA:
5.6		 5u6nA-5coaA:
22.15
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
5ip9 DNA-DIRECTED RNA
POLYMERASE II
SUBUNIT RPB2

(Saccharomyces
cerevisiae) 		PF00562
(RNA_pol_Rpb2_6)
PF04560
(RNA_pol_Rpb2_7)
PF04561
(RNA_pol_Rpb2_2)
PF04563
(RNA_pol_Rpb2_1)
PF04565
(RNA_pol_Rpb2_3)
PF04566
(RNA_pol_Rpb2_4)
PF04567
(RNA_pol_Rpb2_5) 		4 SER B 242
HIS B 363
PHE B 377
MET B 381
 None
 1.47A 5u6nA-5ip9B:
undetectable		 5u6nA-5ip9B:
16.18
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
5k8d COAGULATION FACTOR
VIII,IG GAMMA-1
CHAIN C REGION

(Homo sapiens) 		PF00754
(F5_F8_type_C)
PF07731
(Cu-oxidase_2) 		4 SER B2296
HIS B2031
GLN B2036
MET B2079
 None
 1.49A 5u6nA-5k8dB:
undetectable		 5u6nA-5k8dB:
19.84
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
5kh5 ISOPRENYL
TRANSFERASE

(Streptococcus
pneumoniae) 		PF01255
(Prenyltransf) 		4 PHE A  94
GLN A  53
MET A  27
THR A  56
 None
 1.23A 5u6nA-5kh5A:
undetectable		 5u6nA-5kh5A:
21.78
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
5n5n TUBULIN BETA CHAIN

(Homo sapiens) 		no annotation 4 HIS B   6
PHE B  20
GLN B 136
THR B 239
 None
 1.40A 5u6nA-5n5nB:
2.7		 5u6nA-5n5nB:
22.15
Available target for annotated drug, i.e. matched protein structure has more than 30% sequence identity to known drug target.
5u6s UDP-GLYCOSYLTRANSFER
ASE 74F2

(Arabidopsis
thaliana) 		PF00201
(UDPGT) 		7 HIS A  18
PHE A 113
GLN A 134
MET A 183
MET A 274
TRP A 364
THR A 365
 7WV  A 504 (-4.6A)
7WV  A 504 (-4.8A)
7WV  A 504 (-3.0A)
None
7WV  A 504 ( 3.5A)
BGC  A 501 (-4.2A)
7WV  A 504 (-3.2A)
 0.31A 5u6nA-5u6sA:
66.1		 5u6nA-5u6sA:
99.78
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
5xhq APOLIPOPROTEIN
N-ACYLTRANSFERASE

(Escherichia
coli) 		PF00795
(CN_hydrolase) 		4 SER A  56
PHE A 108
GLN A  45
TRP A 116
 None
 1.36A 5u6nA-5xhqA:
undetectable		 5u6nA-5xhqA:
22.42
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
5xog DNA-DIRECTED RNA
POLYMERASE SUBUNIT
BETA

(Komagataella
phaffii) 		PF00562
(RNA_pol_Rpb2_6)
PF04560
(RNA_pol_Rpb2_7)
PF04561
(RNA_pol_Rpb2_2)
PF04563
(RNA_pol_Rpb2_1)
PF04565
(RNA_pol_Rpb2_3)
PF04566
(RNA_pol_Rpb2_4)
PF04567
(RNA_pol_Rpb2_5) 		4 SER B 233
HIS B 356
PHE B 370
MET B 374
 None
 1.24A 5u6nA-5xogB:
undetectable		 5u6nA-5xogB:
16.26
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
5zov -

(-) 		no annotation 4 HIS A 194
GLN A 196
MET A 413
MET A 131
 ASC  A 501 (-4.0A)
None
None
None
 1.42A 5u6nA-5zovA:
undetectable		 5u6nA-5zovA:
undetectable
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
6au1 PUTATIVE HEMIN
STORAGE PROTEIN

(Bordetella
bronchiseptica) 		no annotation 4 HIS A 473
GLN A 324
MET A 579
TRP A 619
 EDO  A 705 ( 4.6A)
None
None
None
 1.44A 5u6nA-6au1A:
3.5		 5u6nA-6au1A:
12.71
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
6cjb CYSTATHIONINE
BETA-LYASE

(Legionella
pneumophila) 		no annotation 4 SER A 148
GLN A 287
MET A 295
MET A  97
 None
 1.44A 5u6nA-6cjbA:
3.4		 5u6nA-6cjbA:
8.82
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
6eon BETA-GALACTOSIDASE

(Bacteroides
thetaiotaomicron) 		no annotation 4 SER A 317
HIS A 267
PHE A 265
THR A 721
 None
None
GLA  A 801 (-4.7A)
CA  A 802 (-3.6A)
 1.31A 5u6nA-6eonA:
3.6		 5u6nA-6eonA:
21.04
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
6exv DNA-DIRECTED RNA
POLYMERASE II
SUBUNIT RPB2

(Sus scrofa) 		no annotation 4 SER B 223
HIS B 350
PHE B 364
MET B 368
 None
 1.33A 5u6nA-6exvB:
undetectable		 5u6nA-6exvB:
9.80